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Information on EC 2.8.3.6 - 3-oxoadipate CoA-transferase and Organism(s) Pseudomonas putida and UniProt Accession P0A102

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.3 CoA-transferases
                2.8.3.6 3-oxoadipate CoA-transferase
IUBMB Comments
The enzyme, often found in soil bacteria and fungi, is involved in the catabolism of a variety of aromatic compounds, including catechol and protocatechuate, which are degraded via 3-oxoadipate.
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas putida
UNIPROT: P0A102 not found.
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas putida
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
pcaij, 3-oxoadipate coa-transferase, beta-ketoadipate succinyl-coenzyme a transferase, beta-ketoadipate succinyl-coa transferase, beta-ketoadipate:succinyl-coenzyme a transferase, succinyl-coa:3-oxoadipate coa-transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-oxoadipate coenzyme A-transferase
-
-
-
-
3-oxoadipate succinyl-CoA transferase
-
-
-
-
beta-ketoadipate:succinyl-coenzyme A transferase
-
-
coenzyme A-transferase, 3-oxoadipate
-
-
-
-
thiophorase beta-ketoadipate succinyl CoA transferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:3-oxoadipate CoA-transferase
The enzyme, often found in soil bacteria and fungi, is involved in the catabolism of a variety of aromatic compounds, including catechol and protocatechuate, which are degraded via 3-oxoadipate.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-16-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + 3-oxoadipate
?
show the reaction diagram
succinyl-CoA + 3-oxoadipate
succinate + 3-oxoadipyl-CoA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + 3-oxoadipate
?
show the reaction diagram
succinyl-CoA + 3-oxoadipate
succinate + 3-oxoadipyl-CoA
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
-
modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PCAI_PSEPU
231
0
24234
Swiss-Prot
-
PCAJ_PSEPU
213
0
22552
Swiss-Prot
-
A0A1L5PR42_PSEPU
218
0
23223
TrEMBL
-
A0A1X0Z686_PSEPU
231
0
24305
TrEMBL
-
A0A8F8BU41_PSEPU
218
0
23239
TrEMBL
-
A0A2S3WDK3_PSEPU
218
0
23157
TrEMBL
-
A0A4R5SSC6_PSEPU
231
0
24392
TrEMBL
-
A0A083UKI5_PSEPU
291
0
31789
TrEMBL
-
A0A1X0ZST9_PSEPU
293
0
32023
TrEMBL
-
A0A5F0WK27_PSEPU
231
0
24357
TrEMBL
-
A0A0M3CP86_PSEPU
230
0
24249
TrEMBL
-
A0A1X0ZYJ4_PSEPU
259
0
27115
TrEMBL
-
A0A427QBL8_PSEPU
282
0
30885
TrEMBL
-
A0A6I6XZG5_PSEPU
231
0
24356
TrEMBL
-
A0A1Y3KI42_PSEPU
213
0
22566
TrEMBL
-
A0A0P7D1I4_PSEPU
232
0
25081
TrEMBL
-
A0A2Z4RUI1_PSEPU
293
0
32211
TrEMBL
-
A0A1G6EH68_PSEPU
285
0
30920
TrEMBL
-
A0A177Z8Q9_PSEPU
231
0
24380
TrEMBL
-
A0A177SM64_PSEPU
232
0
25103
TrEMBL
-
A0A427QBB3_PSEPU
259
0
27033
TrEMBL
-
A0A126S718_PSEPU
232
0
24921
TrEMBL
-
A0A2C5W3M5_PSEPU
221
1
23439
TrEMBL
-
A0A1X0ZDD6_PSEPU
291
0
31828
TrEMBL
-
A0A6S5TU06_PSEPU
259
0
27057
TrEMBL
-
A0A083UJ77_PSEPU
259
0
27048
TrEMBL
-
A0A083UKD5_PSEPU
230
0
24169
TrEMBL
-
A0A7H5R404_PSEPU
291
0
31843
TrEMBL
-
A0A177SNZ8_PSEPU
231
0
24330
TrEMBL
-
A0A1Y5KLX8_PSEPU
231
0
24333
TrEMBL
-
A0A1X1A457_PSEPU
213
0
22569
TrEMBL
-
A0A8I1EWU2_PSEPU
219
0
22981
TrEMBL
-
A0A7W2KZ26_PSEPU
259
0
27012
TrEMBL
-
A0A2Z4RIQ7_PSEPU
262
0
28893
TrEMBL
-
A0A0M3CRF4_PSEPU
213
0
22596
TrEMBL
-
A0A1B2F7B6_PSEPU
259
0
26988
TrEMBL
-
A0A3M8S6P3_PSEPU
218
0
23239
TrEMBL
-
A0A6S5TH67_PSEPU
267
0
29322
TrEMBL
-
A0A2Z4RSE9_PSEPU
259
0
26879
TrEMBL
-
A0A2Z4RQH2_PSEPU
285
0
30932
TrEMBL
-
A0A177Z749_PSEPU
232
0
25137
TrEMBL
-
A0A177Z777_PSEPU
218
0
23253
TrEMBL
-
A0A059V1R6_PSEPU
232
0
25097
TrEMBL
-
A0A7W2QJI7_PSEPU
232
0
25107
TrEMBL
-
A0A1X1A2V4_PSEPU
232
0
25081
TrEMBL
-
A0A2D1T6Y4_PSEPU
213
0
22418
TrEMBL
-
A0A1X0ZT68_PSEPU
267
0
29340
TrEMBL
-
A0A177SNM7_PSEPU
213
0
22540
TrEMBL
-
A0A0M3D0A6_PSEPU
221
1
23464
TrEMBL
-
A0A2S3X427_PSEPU
232
0
24993
TrEMBL
-
A0A1L7NDA6_PSEPU
232
0
25109
TrEMBL
-
A0A4R5SIS4_PSEPU
213
0
22609
TrEMBL
-
A0A1Q9QY61_PSEPU
213
0
22498
TrEMBL
-
A0A2D1TL91_PSEPU
232
0
25131
TrEMBL
-
A0A4D6XE22_PSEPU
231
0
24447
TrEMBL
-
A0A5F0WHN0_PSEPU
213
0
22620
TrEMBL
-
A0A2S3WI06_PSEPU
259
0
27119
TrEMBL
-
A0A2D1TLC8_PSEPU
218
0
23252
TrEMBL
-
M5AXK7_PSEPU
232
0
25114
TrEMBL
-
A0A1Y3KAZ0_PSEPU
218
0
23157
TrEMBL
-
A0A166J916_PSEPU
218
0
23169
TrEMBL
-
M1M5F3_PSEPU
259
0
27029
TrEMBL
-
A0A8I1EWP9_PSEPU
232
0
24999
TrEMBL
-
A0A8I0W0L2_PSEPU
291
0
31888
TrEMBL
-
A0A1X0ZYS3_PSEPU
286
0
31067
TrEMBL
-
A0A1Y3LAV9_PSEPU
219
0
23302
TrEMBL
-
A0A4R5SR01_PSEPU
218
0
23259
TrEMBL
-
A0A083UJ76_PSEPU
285
0
30964
TrEMBL
-
A0A1Y3KH71_PSEPU
291
0
31863
TrEMBL
-
A0A2S3X3Q1_PSEPU
213
0
22528
TrEMBL
-
A0A166J908_PSEPU
232
0
25109
TrEMBL
-
A0A8I0Z2V8_PSEPU
232
0
25095
TrEMBL
-
A0A8I1EE74_PSEPU
213
0
22606
TrEMBL
-
A0A5P1QXI5_PSEPU
213
0
22574
TrEMBL
-
A0A7Y7MCW4_PSEPU
213
0
22556
TrEMBL
-
M1LCV9_PSEPU
276
0
29987
TrEMBL
-
A0A177SM79_PSEPU
218
0
23210
TrEMBL
-
A0A379KPM2_PSEPU
232
0
25121
TrEMBL
-
A0A179RID9_PSEPU
213
0
22552
TrEMBL
-
A0A0D1M1N4_PSEPU
259
0
26950
TrEMBL
-
A0A1B2F599_PSEPU
232
0
25052
TrEMBL
-
A0A7W2QJK8_PSEPU
218
0
23264
TrEMBL
-
A0A427Q2Y6_PSEPU
232
0
25080
TrEMBL
-
A0A0N8HDQ4_PSEPU
218
0
23248
TrEMBL
-
A0A2S3X3S6_PSEPU
231
0
24312
TrEMBL
-
A0A083UKD4_PSEPU
213
0
22655
TrEMBL
-
A0A7C8XU52_PSEPU
215
0
23246
TrEMBL
-
A0A2C5W0C1_PSEPU
232
0
25094
TrEMBL
-
A0A1B2F607_PSEPU
267
0
29396
TrEMBL
-
A0A1L7NCY7_PSEPU
291
0
31935
TrEMBL
-
A0A2S3WDP9_PSEPU
232
0
24847
TrEMBL
-
A0A379KX12_PSEPU
213
0
22512
TrEMBL
-
A0A8I0Z2H5_PSEPU
291
0
31877
TrEMBL
-
A0A8I1ETT3_PSEPU
230
0
24352
TrEMBL
-
A0A0D1MGJ0_PSEPU
232
0
25057
TrEMBL
-
A0A1X0ZUI7_PSEPU
218
0
23251
TrEMBL
-
A0A1Y3L8U3_PSEPU
220
0
23961
TrEMBL
-
A0A3M8T255_PSEPU
259
0
27028
TrEMBL
-
A0A1L7NDB6_PSEPU
218
0
23204
TrEMBL
-
A0A1B2F727_PSEPU
286
0
31113
TrEMBL
-
A0A2C5W943_PSEPU
259
0
26958
TrEMBL
-
A0A2S3X4B2_PSEPU
218
0
23257
TrEMBL
-
A0A1L7NFU0_PSEPU
231
0
24291
TrEMBL
-
A0A524BUZ7_PSEPU
232
0
25151
TrEMBL
-
A0A083UNF1_PSEPU
221
1
23469
TrEMBL
-
A0A126S5L2_PSEPU
218
0
23225
TrEMBL
-
A0A6S5THU0_PSEPU
218
0
23252
TrEMBL
-
A0A083UNF2_PSEPU
232
0
25174
TrEMBL
-
A0A1X1A2N5_PSEPU
218
0
23253
TrEMBL
-
A0A7U6M4G0_PSEPU
213
0
22467
TrEMBL
-
A0A2Z4RW35_PSEPU
221
0
23423
TrEMBL
-
A0A0P7CFC6_PSEPU
231
0
24305
TrEMBL
-
A0A379KPM1_PSEPU
218
1
23193
TrEMBL
-
A0A7W2KYZ7_PSEPU
286
0
31031
TrEMBL
-
A0A2S3WI05_PSEPU
286
0
31038
TrEMBL
-
A0A1B2F547_PSEPU
218
0
23251
TrEMBL
-
A0A126S0H5_PSEPU
286
0
31117
TrEMBL
-
A0A4D6XAH3_PSEPU
213
0
22499
TrEMBL
-
A0A161YLV8_PSEPU
231
0
24319
TrEMBL
-
A0A059V221_PSEPU
218
0
23237
TrEMBL
-
A0A7W2L652_PSEPU
240
0
25634
TrEMBL
-
A0A4D6XB43_PSEPU
265
0
28955
TrEMBL
-
A0A0M3CXN4_PSEPU
232
0
25158
TrEMBL
-
A0A7C8TX47_PSEPU
259
0
27072
TrEMBL
-
A0A7H5R6N1_PSEPU
213
0
22556
TrEMBL
-
A0A059V2B4_PSEPU
286
0
31002
TrEMBL
-
A0A379KPC1_PSEPU
213
0
22456
TrEMBL
-
A0A0D1PEN3_PSEPU
286
0
30916
TrEMBL
-
M5B4L8_PSEPU
218
0
23271
TrEMBL
-
A0A1Q9R5T4_PSEPU
218
0
23254
TrEMBL
-
A0A0D1LN96_PSEPU
218
0
23143
TrEMBL
-
A0A0P7DKP8_PSEPU
213
0
22511
TrEMBL
-
A0A160GMM6_PSEPU
218
0
23292
TrEMBL
-
A0A1Y5KNN4_PSEPU
232
0
25125
TrEMBL
-
A0A179R7K4_PSEPU
218
0
23239
TrEMBL
-
A0A379KQV1_PSEPU
231
0
24455
TrEMBL
-
A0A1Q9R5T8_PSEPU
269
0
29293
TrEMBL
-
A0A0M3CXR2_PSEPU
291
0
31813
TrEMBL
-
A0A2D1T6X6_PSEPU
231
0
24309
TrEMBL
-
A0A1X0ZU40_PSEPU
232
0
25066
TrEMBL
-
A0A7W2L606_PSEPU
229
0
24095
TrEMBL
-
A0A7U6M4F1_PSEPU
249
0
26337
TrEMBL
-
A0A8I1ETP5_PSEPU
218
0
23084
TrEMBL
-
A0A8I1EFY2_PSEPU
231
0
24349
TrEMBL
-
A0A4D6XAY7_PSEPU
291
0
31844
TrEMBL
-
A0A6S5DCR9_PSEPU
293
0
32185
TrEMBL
-
A0A1Q9QY83_PSEPU
231
0
24314
TrEMBL
-
A0A179R921_PSEPU
232
0
25111
TrEMBL
-
A0A3E1UXF1_PSEPU
286
0
30992
TrEMBL
-
A0A4V3AIM1_PSEPU
232
0
25093
TrEMBL
-
A0A4D6X9V5_PSEPU
232
0
25051
TrEMBL
-
A0A6I6XWZ5_PSEPU
213
0
22454
TrEMBL
-
A0A1B2F5X3_PSEPU
293
0
32103
TrEMBL
-
A0A2S3WN21_PSEPU
231
0
24347
TrEMBL
-
A0A6I6XX91_PSEPU
218
0
23268
TrEMBL
-
A0A6S5CUK1_PSEPU
286
0
31141
TrEMBL
-
A0A126S0H4_PSEPU
259
0
27046
TrEMBL
-
A0A2Z4RTK7_PSEPU
232
0
25248
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
109000
-
gel filtration
24200
-
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE
25300
-
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE
additional information
-
amino acid composition
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
t1/2: 26 min
45
-
t1/2: 4 min
50
-
t1/2: 1 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in the absence of sulfhydryl compounds, DTT stabilizes, 80% residual activity after 5h at 0°C in 0.00086 mM DTT
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, in the presence of DTT, 20% loss of activity within 5 h
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
-
pcaI and pcaJ-genes encoding the two subunits of the enzyme, expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yeh, W.K.; Ornston, L.N.
p-Chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl CoA: beta-ketoadipate CoA transferase
Arch. Microbiol.
138
102-105
1984
Acinetobacter calcoaceticus, Pseudomonas putida
Manually annotated by BRENDA team
Yeh, W.K.; Ornston, L.N.
Evolutionarily homologous alpha 2 beta 2 oligomeric structures in beta-ketoadipate succinyl-CoA transferases from Acinetobacter calcoaceticus and Pseudomonas putida
J. Biol. Chem.
256
1565-1569
1981
Acinetobacter calcoaceticus, Pseudomonas putida
Manually annotated by BRENDA team
Yeh, W.K.; Ornston, L.N.
Similar structures in gamma-carboxymuconolactone decarboxylase and beta-ketoadipate succinyl coenzyme A transferase
J. Bacteriol.
149
374-377
1982
Pseudomonas putida
Manually annotated by BRENDA team
Parales, R.E.; Harwood, C.S.
Characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme A transferase in Pseudomonas putida
J. Bacteriol.
174
4657-4666
1992
Pseudomonas putida, Pseudomonas putida (Q01103 and P0A102)
Manually annotated by BRENDA team
Okamura-Abe, Y.; Abe, T.; Nishimura, K.; Kawata, Y.; Sato-Izawa, K.; Otsuka, Y.; Nakamura, M.; Kajita, S.; Masai, E.; Sonoki, T.; Katayama, Y.
Beta-ketoadipic acid and muconolactone production from a lignin-related aromatic compound through the protocatechuate 3,4-metabolic pathway
J. Biosci. Bioeng.
121
652-658
2016
Pseudomonas putida, Pseudomonas putida KT 2240
Manually annotated by BRENDA team