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Information on EC 2.8.3.26 - succinyl-CoA:mesaconate CoA transferase and Organism(s) Haloarcula hispanica and UniProt Accession G0HQ31

for references in articles please use BRENDA:EC2.8.3.26
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EC Tree
IUBMB Comments
The enzyme participates in the methylaspartate cycle, an anaplerotic pathway that operates in some members of the haloarchaea and forms malate from acetyl-CoA.
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This record set is specific for:
Haloarcula hispanica
UNIPROT: G0HQ31
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The taxonomic range for the selected organisms is: Haloarcula hispanica
The expected taxonomic range for this enzyme is: Haloarcula
Synonyms
MCT, mesaconate:succinyl-CoA CoA transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mesaconate:succinyl-CoA CoA transferase
-
SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:mesaconate CoA transferase
The enzyme participates in the methylaspartate cycle, an anaplerotic pathway that operates in some members of the haloarchaea and forms malate from acetyl-CoA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acrylate + succinyl-CoA
? + succinate
show the reaction diagram
low activity
-
-
?
glutarate + succinyl-CoA
? + succinate
show the reaction diagram
low activity
-
-
?
itaconate + succinyl-CoA
? + succinate
show the reaction diagram
low activity
-
-
?
mesaconate + succinyl-CoA
2-methylfumaryl-CoA + succinate
show the reaction diagram
methylsuccinate + succinyl-CoA
? + succinate
show the reaction diagram
high activity, not a natural substrate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
mesaconate + succinyl-CoA
2-methylfumaryl-CoA + succinate
show the reaction diagram
the enzyme is involved in the methylaspartate cycle
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
the enzyme is dependent on the presence of KCl and shows highest activity at 4 M KCl
additional information
no requirement for divalent cations
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
5 mM, 5% inhibition
CoCl2
5 mM, 10% inhibition
MgCl2
5 mM, 7% inhibition
MnCl2
5 mM, 24% inhibition
NiCl2
5 mM, 5% inhibition
additional information
no inhibition by EDTA
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39.3
Acrylate
37°C, pH 7.8
40.9
Glutarate
37°C, pH 7.8
1.3 - 7.1
mesaconate
0.6 - 2.9
Methylsuccinate
2.8
succinyl-CoA
37°C, pH 7.8
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.2
mesaconate
37°C, pH 7.8
12.2
succinyl-CoA
37°C, pH 7.8
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
Acrylate
37°C, pH 7.8
0.32
Glutarate
37°C, pH 7.8
4.6 - 9.8
mesaconate
9.1 - 12.4
Methylsuccinate
17.8
succinyl-CoA
37°C, pH 7.8
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16.9
37°C, pH 7.8
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
calculated from sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the mct gene is mainly transmitted vertically among haloarchaea
metabolism
the enzyme catalyzes a reaction in the methylaspartate cycle
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
71000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 48000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Haloferax volcanii strain H1209 is used for the heterologous expression of the Haloarcula hispanica proteins
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Borjian, F.; Johnsen, U.; Schoenheit, P.; Berg, I.A.
Succinyl-CoA mesaconate CoA-transferase and mesaconyl-CoA hydratase, enzymes of the methylaspartate cycle in Haloarcula hispanica
Front. Microbiol.
8
1683
2017
Haloarcula hispanica (G0HQ31)
Manually annotated by BRENDA team