Information on EC 2.8.3.19 - CoA:oxalate CoA-transferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.8.3.19
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RECOMMENDED NAME
GeneOntology No.
CoA:oxalate CoA-transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + oxalate = acetate + oxalyl-CoA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:oxalate CoA-transferase
The enzymes characterized from the bacteria Escherichia coli and Acetobacter aceti can also use formyl-CoA and oxalate (EC 2.8.3.16, formyl-CoA transferase) or formyl-CoA and acetate, with significantly reduced specific activities.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetate + oxalyl-CoA
acetyl-CoA + oxalate
show the reaction diagram
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-
?
oxalate + acetyl-CoA
oxalyl-CoA + acetate
show the reaction diagram
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r
additional information
?
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the enzyme shows low activity with formyl-CoA and acetate or oxalate, no activity with acetyl-CoA and succinate, formyl-CoA and succinate, or succinyl-CoA and oxalate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetate + oxalyl-CoA
acetyl-CoA + oxalate
show the reaction diagram
P76518
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-
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017
acetyl-CoA
at pH 6.7 and 25°C
22
oxalate
at pH 6.7 and 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
acetyl-CoA
at pH 6.7 and 25°C
15
oxalate
at pH 6.7 and 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
650
acetyl-CoA
at pH 6.7 and 25°C
0.68
oxalate
at pH 6.7 and 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
at pH 6.7 and 25°C
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
x-ray crystallography
homodimer
x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.5, 0.2 M MgCl2, and 20% (w/v) PEG 8000; hanging drop vapor diffusion method, using 0.1 M Tris-NHCl, pH 8.5, 0.2 M MgCl2, and 20% (w/v) PEG 8000
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Cibacron Blue 3GA column chromatography; ammonium sulfate precipitation, Ni-NTA column chromatography, and Cibacron Blue 3GA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) or C41(DE3) cells
Show AA Sequence (297 entries)
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