Information on EC 2.8.3.17 - cinnamoyl-CoA:phenyllactate CoA-transferase

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The expected taxonomic range for this enzyme is: Clostridium sporogenes

EC NUMBER
COMMENTARY hide
2.8.3.17
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RECOMMENDED NAME
GeneOntology No.
cinnamoyl-CoA:phenyllactate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(E)-cinnamoyl-CoA + (R)-phenyllactate = (E)-cinnamate + (R)-phenyllactyl-CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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dehydration
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tropane, piperidine and pyridine alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(E)-cinnamoyl-CoA:(R)-phenyllactate CoA-transferase
3-Phenylproprionate is a better CoA acceptor than (R)-phenyllactate in vitro. The enzyme from Clostridium sporogenes is specific for derivatives of 3-phenylpropionate and 4-phenylbutyrate.
CAS REGISTRY NUMBER
COMMENTARY hide
289682-21-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E)-cinnamoyl-CoA + (R)-phenyllactate
(E)-cinnamate + (R)-phenyllactoyl-CoA
show the reaction diagram
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-
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r
(E)-cinnamoyl-CoA + 3-(4-hydroxyphenyl)-propionate
(E)-cinnamate + 3-(4-hydroxyphenyl)-propionyl-CoA
show the reaction diagram
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-
-
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r
(E)-cinnamoyl-CoA + 3-phenylpropionate
(E)-cinnamate + 3-phenylpropionyl-CoA
show the reaction diagram
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3-phenylpropionate is a better acceptor than (R)-phenyllactate
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r
(E)-cinnamoyl-CoA + 4-phenylbutyrate
(E)-cinnamate + 4-phenylbutyryl-CoA
show the reaction diagram
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r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(E)-cinnamoyl-CoA + (R)-phenyllactate
(E)-cinnamate + (R)-phenyllactoyl-CoA
show the reaction diagram
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.019
cinnamoyl-CoA
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cosubstrate phenylpropionate
0.009 - 0.012
phenylpropionate
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cosubstrate cinnamoyl-CoA
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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50 mM potassium phosphate, best buffer
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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alpha, beta, gamma, 1 * 46000, 1 * 43000, 1 * 40000, the 46000 kDa subunit A mediates CoA-transfer to phenyllactate, subunits BC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA
43000
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alpha, beta, gamma, 1 * 46000, 1 * 43000, 1 * 40000, the 46000 kDa subunit A mediates CoA-transfer to phenyllactate, subunits BC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA
46000
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alpha, beta, gamma, 1 * 46000, 1 * 43000, 1 * 40000, the 46000 kDa subunit A mediates CoA-transfer to phenyllactate, subunits BC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA
130000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotrimer
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alpha, beta, gamma, 1 * 46000, 1 * 43000, 1 * 40000, the 46000 kDa subunit A mediates CoA-transfer to phenyllactate, subunits BC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (172 entries)
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