Information on EC 2.8.3.15 - succinyl-CoA:(R)-benzylsuccinate CoA-transferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.3.15
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RECOMMENDED NAME
GeneOntology No.
succinyl-CoA:(R)-benzylsuccinate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
succinyl-CoA + (R)-2-benzylsuccinate = succinate + (R)-2-benzylsuccinyl-CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of CoA
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
toluene degradation to benzoyl-CoA (anaerobic)
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toluene degradation
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Toluene degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:(R)-2-benzylsuccinate CoA-transferase
Involved in anaerobic catabolism of toluene and is a strictly toluene-induced enzyme that catalyses the reversible regio- and enantio-selective synthesis of (R)-2-benzylsuccinyl-CoA. The enzyme from Thauera aromatica is inactive when (R)-benzylsuccinate is replaced by (S)-benzylsuccinate.
CAS REGISTRY NUMBER
COMMENTARY hide
260966-56-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R/S)-phenylsuccinyl-CoA + (R,S)-benzylsuccinate
(R/S)-phenylsuccinate + (R,S)-benzylsuccinyl-CoA
show the reaction diagram
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10% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
(R/S)-phenylsuccinyl-CoA + (R,S)-phenylsuccinate
(R/S)-phenylsuccinate + (R,S)-phenylsuccinyl-CoA
show the reaction diagram
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46% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
maleyl-CoA + (R)-2-benzylsuccinate
maleate + (R)-2-benzylsuccinyl-CoA
show the reaction diagram
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-
-
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r
methylsuccinyl-CoA + (R)-2-benzylsuccinate
methylsuccinate + (R)-2-benzylsuccinyl-CoA
show the reaction diagram
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-
-
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r
succinyl-CoA + (R)-2-benzylsuccinate
succinate + (R)-2-benzylsuccinyl-CoA
show the reaction diagram
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-
-
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r
succinyl-CoA + (R)-benzylsuccinate
succinate + (R)-benzylsuccinyl-CoA
show the reaction diagram
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52% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
succinyl-CoA + (R,S)-benzylsuccinate
succinate + (R,S)-benzylsuccinyl-CoA
show the reaction diagram
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44% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
succinyl-CoA + (R,S)-phenylsuccinate
succinate + 2-phenylsuccinyl-CoA + 3-phenylsuccinyl-CoA
show the reaction diagram
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100% activity
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-
?
succinyl-CoA + (S)-benzylsuccinate
succinate + (S)-benzylsuccinyl-CoA
show the reaction diagram
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36% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
succinyl-CoA + 2-oxoindole-3-acetate
succinate + 2-oxoindole-3-acetyl-CoA
show the reaction diagram
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58% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
succinyl-CoA + benzylmalonate
succinate + benzylmalonyl-CoA
show the reaction diagram
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-
-
-
r
succinyl-CoA + indole-3-acetate
succinate + indole-3-acetyl-CoA
show the reaction diagram
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80% activity compared to succinyl-CoA plus (R,S)-phenylsuccinate
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-
?
succinyl-CoA + methylsuccinate
succinate + methylsuccinyl-CoA
show the reaction diagram
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-
-
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r
succinyl-CoA + phenylsuccinate
succinate + phenylsuccinyl-CoA
show the reaction diagram
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-
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + (R)-2-benzylsuccinate
succinate + (R)-2-benzylsuccinyl-CoA
show the reaction diagram
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-
-
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additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(S)-2-benzylsuccinyl-CoA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
(R)-2-benzylsuccinyl-CoA
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0.16
succinate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.024
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with (R/S)-phenylsuccinyl-CoA and (R,S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication
0.085
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with succinyl-CoA and (S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication
0.103
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with succinyl-CoA and (R,S)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication
0.108
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with (R/S)-phenylsuccinyl-CoA and (R,S)-phenylsuccinate as substrates, at pH 8.0, temperature not specified in the publication
0.122
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with succinyl-CoA and (R)-benzylsuccinate as substrates, at pH 8.0, temperature not specified in the publication
0.136
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with succinyl-CoA and 2-oxo-indole-3-acetate as substrates, at pH 8.0, temperature not specified in the publication
0.188
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with succinyl-CoA and indole-3-acetate as substrates, at pH 8.0, temperature not specified in the publication
0.235
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with succinyl-CoA and(R,S)-phenylsuccinate as substrates, at pH 8.0, temperature not specified in the publication
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
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alpha2beta2, 2 * 44000 + 2 * 45000, SDS-PAGE, electrospray-mass spectroscopy
45000
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alpha2beta2, 2 * 44000 + 2 * 45000, SDS-PAGE, electrospray-mass spectroscopy
215000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 40000, Strep-tagged enzyme, SDS-PAGE
heterotetramer
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alpha2beta2, 2 * 44000 + 2 * 45000, SDS-PAGE, electrospray-mass spectroscopy
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable against oxygen
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
strep-tactin affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
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Show AA Sequence (445 entries)
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