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Information on EC 2.8.3.1 - propionate CoA-transferase and Organism(s) Anaerotignum propionicum and UniProt Accession Q9L3F7
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2.8.3.1 propionate CoA-transferaseIUBMB Comments
Butanoate and lactate can also act as acceptors.
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Word Map
- 2.8.3.1
- polyhydroxyalkanoate
- propionicum
- eutropha
-
polylactic
- homopolymer
- acetoacetyl-coa
- lactyl-coa
- 2-hydroxybutyrate
- elsdenii
- megasphaera
-
phac1s
- 3-hydroxypropionate
-
pane
- 3-hydroxybutyryl-coa
- synthesis
-
2-hydroxyacids
- biotechnology
- analysis
The taxonomic range for the selected organisms is: Anaerotignum propionicum
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
propionyl-coa transferase, propionate coa-transferase, propionate coa transferase, propionate-coa transferase, propionyl-coa:succinate coa transferase, coenzyme-a transferase, propionate coenzyme a transferase, propionate coenzyme a coa transferase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
coenzyme A transferase, propionate
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-
-
-
propionate coenzyme A-transferase
-
-
-
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propionate-CoA:lactoyl-CoA transferase
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-
-
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propionyl CoA:acetate CoA transferase
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-
-
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propionyl-CoA transferase
propionyl-CoA transferase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
coenzyme A transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
KEGG
-
-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:propanoate CoA-transferase
Butanoate and lactate can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY
9026-15-7
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 2-hydroxybutanoate
acetate + 2-hydroxybutyryl-CoA
-
-
-
-
?
acetyl-CoA + 2-hydroxybutyrate
acetate + 2-hydroxybutyryl-CoA
-
-
-
-
?
acetyl-CoA + 3-hydroxybutyrate
acetate + 3-hydroxybutyryl-CoA
-
-
-
-
?
acetyl-CoA + 3-hydroxypropionate
acetate + 3-hydroxypropionyl-CoA
-
-
-
-
?
acetyl-CoA + 3-mercaptopropionate
acetate + 3-mercaptopropionyl-CoA
-
-
-
-
?
acetyl-CoA + lactate
pyruvate + acetate + CoA
-
(R)-lactate preferred over (S)-lactate
-
?
acetyl-CoA + propanoate
acetate + propanoyl-CoA
propionate is contained in various fermented foods, is used as an antimicrobial food additive, accumulation of propionate in the blood is caused by propionic acidemia
-
-
?
acetyl-CoA + propanoate
acetate + propanoyl-CoA
-
best substrate
-
r
acetyl-CoA + propanoate
acetate + propanoyl-CoA
-
specific for monocarboxylic acids
-
r
acetyl-CoA + propanoate
acetate + propanoyl-CoA
-
reaction of (S)-alanine fermentation pathway
-
-
?
additional information
?
-
-
The wild-type Pct is not able to efficiently convert lactate to lactyl-CoA
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + propanoate
acetate + propanoyl-CoA
propionate is contained in various fermented foods, is used as an antimicrobial food additive, accumulation of propionate in the blood is caused by propionic acidemia
-
-
?
additional information
?
-
-
The wild-type Pct is not able to efficiently convert lactate to lactyl-CoA
-
-
?
acetyl-CoA + propanoate
acetate + propanoyl-CoA
-
reaction of (S)-alanine fermentation pathway
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
propionate
increase in propionate concentration lead to increase of enzyme amount
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
development of propionate-detection systems, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
V193A
-
the Pct540 mutant contains the V193A mutation, and four silent nucleotide exchanges of T78C, T669C, A1125G, T1158C
additional information
-
construction of an engineered enzyme mutant, Pct540, for the generation of poly(3-hydroxybutyrate-co-lactate) and lactyl-CoA endogenously, random mutagenesis, overview. Method optimization and molecular weight of synthesized polymers, overview
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of the engineered mutant Pct540 from Clostridium propionicum in Escherichia coli strain XL-1 Blue with engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1 mutant, i.e. PhaC1Ps6-19. The expressed wild-type Pct is not able to efficiently convert lactate to lactyl-CoA and exerts an inhibitory effect on cell growth
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coexpression of the engineered mutant Pct540 from Clostridium propionicum in the engineered Escherichia coli strain JLX7 with engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1 mutant, i.e. PhaC1Ps6-19, production of polylactic acid, flux response analysis, overview. The engineered Escherichia coli strain exerts several knocked out genes, overview
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
propionate-biosensor, using propionate coenzyme A transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana as enzyme electrodes
biotechnology
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establishing of an on-site monitoring of volatile fatty acids VFA, such as propionate, biosensing system using recombinant propionate CoA transferase and recombinant acyl-CoA oxidase, this system produces hydrogen peroxide in the presence of acetyl-CoA, oxygen, and VFA substrates, which can be quantified by colorimetric methods using peroxidase and dye reagents, e.g., 4-aminobenzoic acid plus 4-aminoantipyrine, overview
synthesis
synthesis
-
engineered mutant Pct, coexpressed with an engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1, is able to produce poly(3-hydroxybutyrate-co-lactate) copolymers having 9-64 mol% of lactate. Polylactic acid might be a good alternative to petroleum-based plastic as it possesses several desirable properties such as biodegradability, biocompatibility, compostability, and low toxicity to humans
synthesis
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in an engineered Escherichia coli strain JLX7, the engineered enzyme mutant Pct540, coexpressed with an engineered Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate synthase 1, is able to effectively produce polylactic acid, a promising biomass-derived polymer, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schweiger, G.; Buckel, W.
On the dehydration of (R)-lactate in the fermentation of alanine to propionate by Clostridium propionicum
FEBS Lett.
171
79-84
1984
Anaerotignum propionicum
Selmer, T.; Willanzheimer, A.; Hetzel, M.
Propionate CoA-transferase from Clostridium propionicum. Cloning of gene and identification of glutamate 324 at the active site
Eur. J. Biochem.
269
372-380
2002
Anaerotignum propionicum
Rajashekhara, E.; Hosoda, A.; Sode, K.; Ikenaga, H.; Watanabe, K.
Volatile fatty acid-sensing system involving coenzyme-A transferase
Biotechnol. Prog.
22
334-337
2006
Anaerotignum propionicum
Sode, K.; Tsugawa, W.; Aoyagi, M.; Rajashekhara, E.; Watanabe, K.
Propionate sensor using coenzyme-A transferase and acyl-CoA oxidase
Protein Pept. Lett.
15
779-781
2008
Anaerotignum propionicum, Anaerotignum propionicum (Q9L3F7)
Yang, T.H.; Kim, T.W.; Kang, H.O.; Lee, S.H.; Lee, E.J.; Lim, S.C.; Oh, S.O.; Song, A.J.; Park, S.J.; Lee, S.Y.
Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase
Biotechnol. Bioeng.
105
150-160
2010
Anaerotignum propionicum
Jung, Y.K.; Kim, T.Y.; Park, S.J.; Lee, S.Y.
Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers
Biotechnol. Bioeng.
105
161-171
2010
Anaerotignum propionicum
Han, X.; Satoh, Y.; Satoh, T.; Matsumoto, K.; Kakuchi, T.; Taguchi, S.; Dairi, T.; Munekata, M.; Tajima, K.
Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type class I PHA synthase from Ralstonia eutropha
Appl. Microbiol. Biotechnol.
92
509-517
2011
Anaerotignum propionicum, Anaerotignum propionicum JCM 1430
Park, S.J.; Kang, K.H.; Lee, H.; Park, A.R.; Yang, J.E.; Oh, Y.H.; Song, B.K.; Jegal, J.; Lee, S.H.; Lee, S.Y.
Propionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli
J. Biotechnol.
165
93-98
2013
Anaerotignum propionicum
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