Information on EC 2.8.2.7 - UDP-N-acetylgalactosamine-4-sulfate sulfotransferase

for references in articles please use BRENDA:EC2.8.2.7
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.7
-
RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylgalactosamine-4-sulfate sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + UDP-N-acetyl-D-galactosamine 4-sulfate = adenosine 3',5'-bisphosphate + UDP-N-acetyl-D-galactosamine 4,6-bissulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:UDP-N-acetyl-D-galactosamine-4-sulfate 6-sulfotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37278-32-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Quail
-
-
Manually annotated by BRENDA team
White Leghorn hen
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + chondroitin
?
show the reaction diagram
3'-phosphoadenylylsulfate + delta4,5-glucuronido-N-acetylgalactosamine 4-sulfate
?
show the reaction diagram
-
poor substrate
-
-
?
3'-phosphoadenylylsulfate + dermatan
?
show the reaction diagram
-
incorporates sulfate exclusively at C4 position of N-acetylgalactosamine residues
-
-
?
3'-phosphoadenylylsulfate + N-acetylgalactosamine 1-phosphate 4-sulfate
adenosine 3',5'-bisphosphate + N-acetylgalactosamine 1,6-bissulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-acetylgalactosamine 4-sulfate
adenosine 3',5'-bisphosphate + N-acetylgalactosamine 4,6-bissulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + UDP-N-acetylgalactosamine 4-sulfate
adenosine 3',5'-bisphosphate + UDP-N-acetylgalactosamine-4,6-bissulfate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + UDP-N-acetylgalactosamine 4-sulfate
adenosine 3',5'-bisphosphate + UDP-N-acetylgalactosamine-4,6-bissulfate
show the reaction diagram
-
component of microsomal multienzyme system involved in UDP-N-acetylgalactosamine 6-sulfate biosynthesis
-
-
?
additional information
?
-
-
biosynthesis of dermatan sulfate
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
enzyme is stimulated at concentrations of 5-20 mM
Mg2+
-
enzyme is stimulated at concentrations of 5-20 mM
Mn2+
-
enzyme is stimulated at concentrations of 5-20 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.56 - 4.22
Chondroitin
-
pH 6, 37C, Km value depends on number of disaccharides of the receptor
2
DELTA4,5-glucuronido-N-acetylgalactosamine 4-sulfate
-
pH 4.8, 38C
0.27 - 0.61
Dermatan
-
pH 6, 37C, Km value depends on number of disaccharides of the receptor
0.13
N-acetylgalactosamine 1-phosphate 4-sulfate
-
pH 4.8, 38C
1.4
N-acetylgalactosamine 4-sulfate
-
pH 4.8, 38C
0.05
UDP-N-acetylgalactosamine 4-sulfate
-
pH 4.8, 38C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000069
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
30% activity at pH 5, 15% activity at pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
predominantly
-
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18C, 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial