Information on EC 2.8.2.6 - choline sulfotransferase

for references in articles please use BRENDA:EC2.8.2.6
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.8.2.6
-
RECOMMENDED NAME
GeneOntology No.
choline sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + choline = adenosine 3',5'-bisphosphate + choline sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:choline sulfotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9047-23-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CMI 38,595 S eta, sulfateless mutant
-
-
Manually annotated by BRENDA team
(Sm.)O.Kuntze
-
-
Manually annotated by BRENDA team
Limonium nashii
Small
-
-
Manually annotated by BRENDA team
Limonium perezii
-
-
-
Manually annotated by BRENDA team
Limonium ramosissimum
G.Donelly
-
-
Manually annotated by BRENDA team
Mill
-
-
Manually annotated by BRENDA team
no activity in Brassica napus
L.cv. Westar
-
-
Manually annotated by BRENDA team
no activity in Brassica oleracea
L.cv April Red
-
-
Manually annotated by BRENDA team
no activity in Helianthus annuus
sunflower, L. cv Sundak
-
-
Manually annotated by BRENDA team
no activity in Hordeum vulgare
barley, L. cv Proctor
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-
Manually annotated by BRENDA team
no activity in Zea mays
maize, L. cv COOP 6309
-
-
Manually annotated by BRENDA team
C12B
-
-
Manually annotated by BRENDA team
C12B
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + choline
adenosine 3',5'-bisphosphate + choline sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + N,N,N-triethylaminoethanol
adenosine 3',5'-bisphosphate + N,N,N-triethylaminoethanol O-sulfate
show the reaction diagram
-
2% of the activity with choline
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-
-
3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol
adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + choline
adenosine 3',5'-bisphosphate + choline sulfate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
not even a partial requirement for Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-Naphthylamine
-
-
2'-AMP
-
weak inhibitor
2,4-dinitrophenol
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3-Aminophenol
-
-
4-Dimethylaminophenol
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-
4-nitrophenol
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competitive inhibition
Acetylcholine
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-
beta-homocholine
-
-
Ca2+
Limonium perezii
-
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carnitine
-
competitive inhibition
Chlorocholine
-
competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
choline
-
substrate inhibitor
choline chloride
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choline-O-phosphate
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dead-end inhibitor, competitive inhibition with choline, linear mixed type inhibitor with respect to PAPS
Co2+
-
sensitive to metal ions, almost complete inhibition at 6.0 mM
diethanolamine
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Dimethylaminoethanethiol
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dimethylaminoethanol
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substrate inhibition
Dimethylaminopropan-1-ol
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competitive inhibition
Dimethylaminopropen-1-ol
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dimethylethylaminoethanol
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substrate inhibition
ethyltrimethylammonium bromide
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gamma-homocholine
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Hexadecyltrimethylammonium bromide
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L-cysteine
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N,N-diethylethanolamine
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N-ethylmaleimide
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n-Propanol
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concentrations above 0.025 M
Na2SO4
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Ni2+
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sensitive to metal ions, almost complete inhibition at 6.0 mM
p-chloromercuribenzoate
phenol
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strong inhibitor
Prostigmine
-
-
Tetramethylammonium
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competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
thiocholine
triethanolamine
-
-
Trimethylammonium
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competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
vinyl trimethylammonium
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neurine, competitive dead-end inhibitor with respect to choline, uncompetitive with respect to PAPS
vinyl trimethylammonium bromide
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-
Zn2+
Limonium perezii
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-
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3'-phosphoadenosine 5'-phosphosulfate
-
choline sulfate formation is increased 5fold by 1.0 mM
adenosine 5'-phosphosulfate
-
choline sulfate formation is increased 2.8fold by 10 mM
n-Propanol
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maximal activation, at 2.5 mM, inhibition above 25 mM
NaCl
Limonium perezii
-
activity is increased at least 4fold by salinization with 40% v/v artificial sea water
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055 - 0.0222
3'-phosphoadenylylsulfate
0.017 - 12
choline
25
dimethylaminoethanol
-
pH 7.3, 37C
20
dimethylethylaminoethanol
-
pH 7.3, 37C
2.8
N,N,N-triethylaminoethanol
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pH 7.8, 37C
0.8
N,N-Dimethylaminoethanol
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pH 7.8, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5 - 14
3',5'-ADP
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pH 7.8, 37C depending on the choline concentration
4.1
3'-AMP
-
pH 7.8, 37C
0.42 - 9.4
choline-O-phosphate
5.8
thiocholine
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pH 7.8, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
Limonium perezii
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
-
about 50% of activity maximum at pH 5.8 and 10.1
7.6 - 9.9
Limonium perezii
-
about 50% of activity maximum at pH 7.6, about 70% of activity maximum at pH 9.9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 12
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stable at room temperature in the pH range 8.0-11.0, above pH 11.0 enzyme is rapidly inactivated, 80% inactivation at pH 12.0, below pH 8.0 stablity declines slowly with decreasing pH, maximum stability at pH 8,5-10.5
643827
7 - 8.8
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stable for at least 15 min
643829
7.6
Limonium perezii
-
retains 50% of its activity
643830
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46
-
no loss of activity during 10 min at pH 9.2, 30% loss at pH 7.3, 34% loss at pH 5.0
55
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almost completely inactivated
58.5
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complete loss of activity at pH 5.0, 85% activity retained at pH 9.2
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
age-inactivated enzyme can be restored to full activity by 10 min preincubation with 50 mM mercaptoethanol, prolonged incubation for 24 h results in irreversible denaturation
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unstable
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 25% sucrose, loses only about 20% of its activity after 1 month
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-20C, freezing and storing for 3 days results in complete loss of activity
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-20C, rapidly loses activity
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0C, purified enzyme relatively stable when stored in presence of 25% sucrose
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2C, more stable after (NH4)2SO4 or acetone fractionation, activity is reduced by 85% during 4 weeks
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2C, very unstable, loses 95% of its activity after 5 days
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4C, rapidly loses activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial