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Information on EC 2.8.2.5 - chondroitin 4-sulfotransferase and Organism(s) Mus musculus and UniProt Accession Q9JME2

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.5 chondroitin 4-sulfotransferase
IUBMB Comments
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
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This record set is specific for:
Mus musculus
UNIPROT: Q9JME2
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
c4st-1, chst11, carbohydrate sulfotransferase, chondroitin 4-sulfotransferase, c4st, c4st-2, chondroitin 4-o-sulfotransferase-1, chondroitin-4-sulfotransferase, chondroitin 4-o-sulfotransferase, chondroitin sulfotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chondroitin 4-O-sulfotransferae-1
-
chondroitin 4-O-sulfotransferase-1
-
chondroitin-4-sulfotransferase
-
chondroitin 4-O-sulfotransferase-1
-
-
chondroitin 4-O-sulfotransferase-2
-
-
chondroitin 4-sulfotransferase 1
-
-
chondroitin 4-sulfotransferase 2
-
-
chondroitin sulfotransferase
-
-
-
-
sulfotransferase, chondroitin
-
-
-
-
sulfotransferase, chondroitin 4-
-
-
-
-
additional information
-
the enzyme belongs to the chondroitin/dermatan 4-O-sulfotransferase, C4ST/D4ST, family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
83589-04-2
-
9026-07-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
adenosine 3',5'-bisphosphate + mixture of 4'-monosulfated oligosaccharides
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergents
-
with the exception of Triton X-100 and Nonidet P-40, inhibit sulfation in the mast cell system
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Nonidet P-40
-
activates at 1%
Triton X-100
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00001
recombinant enzyme, substrate chondroitin
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
assay at
6.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
bone marrow-derived mast cell, 200fold higher expression of mC4ST-1 than in bone marrow cell
Manually annotated by BRENDA team
ventricular zone of the dorsal and the ventral telencephalon
Manually annotated by BRENDA team
of the telencephalon
Manually annotated by BRENDA team
-
C4ST-2 expression occurs in the early stage bone marrow cell cultures, the process of mast cell maturation does not alter the expression, low expression at day 0, followed by an increase in expression at later stages of culture
Manually annotated by BRENDA team
-
developed from bone marrow cells isolated from femur and tibia from C57BL/6 mice, mast cell maturation strongly correlates with the expression of C4ST-1
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHSTB_MOUSE
352
1
41632
Swiss-Prot
Secretory Pathway (Reliability: 3)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R186A
-
the mutant fails to bind 3'-phosphoadenylyl sulfate
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sephadex G-25 chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
genetic library screening, DNA and amino acid sequence determination and analysis, transient functional overexpression of wild-type and FLAG-tagged enzyme in COS-7 cells, control expression of C4ST in antisense orientation
expressed in L cells
-
expression analysis
-
expression of C4ST-1 in enzyme-deficient sog9 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
a decline in arylsulfatase B reduces chondroitin-4-sulfotransferase mRNA expression. Inhibition of bone morphogenetic protein 4 also reduces enzyme expression
silencing Wnt9A increases the expression of CHST11
the suppression of enzyme gene expression by Wnt/b-etacatenin signaling can be recovered by treatment with trichostatin A
A23187 causes a down-regulated expression of the C4ST-2 gene with 50% reduction after 1 h, followed by return to basal levels after 4.5 h
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
gene-trap mutation in bone morphogenic protein-induced enzyme causes severe chondrodysplasia characterised by disorganised cartilage growth plate as well as specific alterations in the orientation of chondrocyte columns. Phenotype is associated with chondroitin sulfation imbalance, mislocalization of chondroitin sulfate and imbalance of apoptotic signals
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sugumaran, G.; Silbert, J.E.
Sulfation of chondroitin. Specificity, degree of sulfation, and detergent effects with 4-sulfating and 6-sulfating microsomal systems
J. Biol. Chem.
263
4673-4678
1988
Mus musculus
Manually annotated by BRENDA team
Sugumaran, G.; Cogburn, J.N.; Silbert, J.E.
Simultaneous sulfation of endogenous chondroitin sulfate and chondroitin-derived oligosaccharides. Studies with separate 4-sulfating and 6-sulfating microsomal systems
J. Biol. Chem.
261
12659-12664
1986
Mus musculus
Manually annotated by BRENDA team
Yamauchi, S.; Mita, S.; Matsubara, T.; Fukuta, M.; Habuchi, H.; Kimata, K.; Habuchi, O.
Molecular cloning and expression of chondroitin 4-sulfotransferase
J. Biol. Chem.
275
8975-8981
2000
Mus musculus (Q9JME2), Mus musculus
Manually annotated by BRENDA team
Kluppel, M.; Wight, T.N.; Chan, C.; Hinek, A.; Wrana, J.L.
Maintenance of chondroitin sulfation balance by chondroitin-4-sulfotransferase 1 is required for chondrocyte development and growth factor signaling during cartilage morphogenesis
Development
132
3989-4003
2005
Mus musculus
Manually annotated by BRENDA team
Uyama, T.; Ishida, M.; Izumikawa, T.; Trybala, E.; Tufaro, F.; Bergstroem, T.; Sugahara, K.; Kitagawa, H.
Chondroitin 4-O-sulfotransferase-1 regulates E disaccharide expression of chondroitin sulfate required for Herpes simplex virus infectivity
J. Biol. Chem.
281
38668-38674
2006
Mus musculus
Manually annotated by BRENDA team
Ohtake, S.; Kondo, S.; Morisaki, T.; Matsumura, K.; Kimata, K.; Habuchi, O.
Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells
Biochim. Biophys. Acta
1780
687-695
2008
Mus musculus (Q9JME2), Mus musculus
Manually annotated by BRENDA team
Akita, K.; von Holst, A.; Furukawa, Y.; Mikami, T.; Sugahara, K.; Faissner, A.
Expression of multiple chondroitin/dermatan sulfotransferases in the neurogenic regions of the embryonic and adult central nervous system implies that complex chondroitin sulfates have a role in neural stem cell maintenance
Stem Cells
26
798-809
2008
Mus musculus (Q9JME2), Mus musculus
Manually annotated by BRENDA team
Duelli, A.; Roennberg, E.; Waern, I.; Ringvall, M.; Kolset, S.O.; Pejler, G.
Mast cell differentiation and activation is closely linked to expression of genes coding for the serglycin proteoglycan core protein and a distinct set of chondroitin sulfate and heparin sulfotransferases
J. Immunol.
183
7073-7083
2009
Mus musculus, Mus musculus C57BL/6
Manually annotated by BRENDA team
Izumikawa, T.; Okuura, Y.; Koike, T.; Sakoda, N.; Kitagawa, H.
Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in co-operation with chondroitin N-acetylgalactosaminyltransferase-2
Biochem. J.
434
321-331
2011
Mus musculus
Manually annotated by BRENDA team
Izumikawa, T.; Koike, T.; Kitagawa, H.
Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1
Biochem. J.
441
697-705
2012
Mus musculus
Manually annotated by BRENDA team
Mizumoto, S.; Watanabe, M.; Yamada, S.; Sugahara, K.
Expression of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase involved in chondroitin sulfate synthesis is responsible for pulmonary metastasis
BioMed Res. Int.
2013
656319
2013
Mus musculus (Q9JME2)
Manually annotated by BRENDA team
Nadanaka, S.; Kinouchi, H.; Kitagawa, H.
Histone deacetylase-mediated regulation of chondroitin 4-O-sulfotransferase-1 (Chst11) gene expression by Wnt/beta-catenin signaling
Biochem. Biophys. Res. Commun.
480
234-240
2016
Mus musculus (Q9JME2)
Manually annotated by BRENDA team
Bhattacharyya, S.; Feferman, L.; Tobacman, J.K.
Regulation of chondroitin-4-sulfotransferase (CHST11) expression by opposing effects of arylsulfatase B on BMP4 and Wnt9A
Biochim. Biophys. Acta
1849
342-352
2015
Homo sapiens (Q9NPF2), Homo sapiens, Mus musculus (Q9JME2)
Manually annotated by BRENDA team