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EC Tree
IUBMB Comments The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
The taxonomic range for the selected organisms is: Rattus norvegicus The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
c4st-1, chst11, carbohydrate sulfotransferase, c4st, chondroitin 4-sulfotransferase, c4st-2, chondroitin-4-sulfotransferase, chondroitin 4-o-sulfotransferase-1, chondroitin 4-o-sulfotransferase, chondroitin sulfotransferase,
more
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carbohydrate (chondroitin-4)sulfotransferase 11
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chondroitin 4-sulfotransferase 1
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chondroitin sulfotransferase
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-
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sulfotransferase, chondroitin
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-
-
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sulfotransferase, chondroitin 4-
-
-
-
-
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sulfate group transfer
-
-
-
-
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3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
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3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
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low activity
-
-
?
3'-phosphoadenylylsulfate + desulfated chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A
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low activity
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
-
-
-
-
?
additional information
?
-
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
-
-
-
?
additional information
?
-
-
no activity with dermatan sulfate, keratan sulfate
-
-
?
additional information
?
-
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chondroitin sulfate E, heparan sulfate, and completely desulfated N-resulfated heparin are no acceptor substrates
-
-
?
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3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
-
-
?
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Ca2+
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highest stimulatory effect, optimal concentration: 5 mM
Mg2+
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slightly stimulating
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2-mercaptoethanol
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stimulates
dithiothreitol
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activation of the purified enzyme
glutathione
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reduced glutathione stimulates
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0.00027 - 0.0011
3'-phosphoadenylylsulfate
0.00027
3'-phosphoadenylylsulfate
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pH 6.8, 37°C, in presence of protamine
0.0005
3'-phosphoadenylylsulfate
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pH 6.8, 37°C, wild-type
0.0011
3'-phosphoadenylylsulfate
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pH 6.8, 37°C, mutant with deletion of C-terminal glycosylation site
2.1
chondroitin
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pH 6.8, 37°C, wild-type
2.7
chondroitin
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pH 6.8, 37°C, mutant with deletion of C-terminal glycosylation site
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0.000006
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purified enzyme
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UniProt
brenda
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chondrosarcoma cell line
brenda
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brenda
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extracellular, secretion to the medium
brenda
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enzyme is released into the cell culture medium
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brenda
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-
-
brenda
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CHSTB_RAT
352
1
41626
Swiss-Prot
Secretory Pathway (Reliability: 3 )
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35000
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1 * 35000, deglycosylated protein, SDS-PAGE
50000
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about, gel filtration
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additional information
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the deglycosylated enzyme forms dimers in solution of 70 kDa
monomer
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1 * 35000, deglycosylated protein, SDS-PAGE
monomer
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1 * 50000-54000, nonreducing conditions, SDS-PAGE
monomer
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1 * 60000-64000, reducing conditions, SDS-PAGE
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glycoprotein
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enzyme contains N-linked oligosaccharides
glycoprotein
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contains about 35% N-linked oligosaccharides, treatment of enzyme with peptide N-glycosidase F causes a marked decrease in activity, production of active form of recombinant enzyme is inhibited by tunicamycin
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additional information
reconstitution of the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source, method optimization, overview. Specific sulfation transformation systems are successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV, EC 2.8.2.1), chondroitin 4-sulfotransferase (C4ST) and chondroitin 6-sulfotransferase (C6ST, EC 2.8.2.17). Chondroitin sulfate A and C are enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The ASST IV enzyme is used for PAPS regeneration. Purification of CS disaccharides by anion exchange chrmatography and NMR analysis of the composition of CS disaccharides
additional information
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deletion of N-glycosylation sites, loss of the N-glycosylation site at the C-terminus reduces activity by 80%
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-20°C, purified enzyme, loss of 80% activity
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1920fold, to homogeneity, from the serum-free cell culture medium
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gene C4ST, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with ASSTIV and C6ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification
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traumatic brain injury leads to an increase in mRNA expression
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synthesis
the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with aryl sulfotransferase IV and chondroitin 6-sulfotransferase, overview
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Yamauchi, S.; Hirahara, Y.; Usui, H.; Takeda, Y.; Hoshino, M.; Fukuta, M.; Kimura, J.H.; Habuchi, O.
Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line
J. Biol. Chem.
274
2456-2463
1999
Rattus norvegicus
brenda
Yusa, A.; Kitajima, K.; Habuchi, O.
N-linked oligosaccharides are required to produce and stabilize the active form of chondroitin 4-sulphotransferase-1
Biochem. J.
388
115-121
2005
Rattus norvegicus
brenda
Bhattacharyya, S.; Zhang, X.; Feferman, L.; Johnson, D.; Tortella, F.C.; Guizzetti, M.; Tobacman, J.K.
Decline in arylsulfatase B and increase in chondroitin 4-sulfotransferase combine to increase chondroitin 4-sulfate in traumatic brain injury
J. Neurochem.
134
728-739
2015
Rattus norvegicus (P69478)
brenda
Zhou, Z.; Li, Q.; Huang, H.; Wang, H.; Wang, Y.; Du, G.; Chen, J.; Kang, Z.
A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans
Biotechnol. Bioeng.
115
1561-1570
2018
Rattus norvegicus (P69478)
brenda