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Information on EC 2.8.2.5 - chondroitin 4-sulfotransferase and Organism(s) Rattus norvegicus and UniProt Accession P69478

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.5 chondroitin 4-sulfotransferase
IUBMB Comments
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
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This record set is specific for:
Rattus norvegicus
UNIPROT: P69478
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
c4st-1, chst11, carbohydrate sulfotransferase, c4st, chondroitin 4-sulfotransferase, c4st-2, chondroitin-4-sulfotransferase, chondroitin 4-o-sulfotransferase-1, chondroitin 4-o-sulfotransferase, chondroitin sulfotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbohydrate (chondroitin-4)sulfotransferase 11
-
chondroitin 4-sulfotransferase 1
-
chondroitin sulfotransferase
-
-
-
-
sulfotransferase, chondroitin
-
-
-
-
sulfotransferase, chondroitin 4-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
83589-04-2
-
9026-07-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + desulfated chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Ca2+
-
highest stimulatory effect, optimal concentration: 5 mM
Fe2+
-
stimulates
Mg2+
-
slightly stimulating
Mn2+
-
stimulates
Sr2+
-
stimulates
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
stimulates
dithiothreitol
-
activation of the purified enzyme
glutathione
-
reduced glutathione stimulates
protamine
-
stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00027 - 0.0011
3'-phosphoadenylylsulfate
2.1 - 2.7
chondroitin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000006
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
chondrosarcoma cell line
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
enzyme is released into the cell culture medium
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHSTB_RAT
352
1
41626
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
1 * 35000, deglycosylated protein, SDS-PAGE
50000
-
about, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
the deglycosylated enzyme forms dimers in solution of 70 kDa
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified enzyme, loss of 80% activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1920fold, to homogeneity, from the serum-free cell culture medium
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene C4ST, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with ASSTIV and C6ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
traumatic brain injury leads to an increase in mRNA expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with aryl sulfotransferase IV and chondroitin 6-sulfotransferase, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamauchi, S.; Hirahara, Y.; Usui, H.; Takeda, Y.; Hoshino, M.; Fukuta, M.; Kimura, J.H.; Habuchi, O.
Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line
J. Biol. Chem.
274
2456-2463
1999
Rattus norvegicus
Manually annotated by BRENDA team
Yusa, A.; Kitajima, K.; Habuchi, O.
N-linked oligosaccharides are required to produce and stabilize the active form of chondroitin 4-sulphotransferase-1
Biochem. J.
388
115-121
2005
Rattus norvegicus
Manually annotated by BRENDA team
Bhattacharyya, S.; Zhang, X.; Feferman, L.; Johnson, D.; Tortella, F.C.; Guizzetti, M.; Tobacman, J.K.
Decline in arylsulfatase B and increase in chondroitin 4-sulfotransferase combine to increase chondroitin 4-sulfate in traumatic brain injury
J. Neurochem.
134
728-739
2015
Rattus norvegicus (P69478)
Manually annotated by BRENDA team
Zhou, Z.; Li, Q.; Huang, H.; Wang, H.; Wang, Y.; Du, G.; Chen, J.; Kang, Z.
A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans
Biotechnol. Bioeng.
115
1561-1570
2018
Rattus norvegicus (P69478)
Manually annotated by BRENDA team