Information on EC 2.8.2.31 - petromyzonol sulfotransferase

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The expected taxonomic range for this enzyme is: Petromyzon marinus

EC NUMBER
COMMENTARY hide
2.8.2.31
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RECOMMENDED NAME
GeneOntology No.
petromyzonol sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + 5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol = adenosine 3',5'-bisphosphate + 5alpha-cholan-3alpha,7alpha,12alpha-triol 24-sulfate
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol sulfotransferase
The enzyme from the lamprey Petromyzon marinus can also use the corresponding 3-ketone as a substrate. It is stereoselective (5alpha-cholane) and regioselective, exhibiting a preference for an hydroxy group at C-24. The enzyme is inactive when allocholic acid, which has a carboxy group at C-24, is used as a substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-76-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 5alpha-cholan-3alpha,7alpha,12alpha, 24-tetrol
adenosine 3',5'-bisphosphate + 5alpha-cholan-3alpha,7alpha,12alpha-triol 24-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7alpha,12alpha,24-trihydroxy-5alpha-cholan-3-one
adenosine 3',5'-bisphosphate + 7alpha,12alpha-dihydroxy-5alpha-cholan-3-one 24-sulfate
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0025
3'-phosphoadenosine 5'-phosphosulfate
-
22C, pH 8.0
0.008
sinapic acid methyl ester
-
22C, pH 8.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000002
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 11
-
approx. 25% of maximal activity at pH 6.0 and pH 11.0, respectively
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 60
-
approx. 60% of maximal activity at 10C, approx. 10% of maximal activity at 40C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
larval liver
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
x * 47000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 47000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE column, gel filtration, 3'-phosphoadenosine 5'-phosphate affinity chromatography
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