Information on EC 2.8.2.28 - quercetin-3,3'-bissulfate 7-sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.28
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RECOMMENDED NAME
GeneOntology No.
quercetin-3,3'-bissulfate 7-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + quercetin 3,3'-bissulfate = adenosine 3',5'-bisphosphate + quercetin 3,7,3'-trissulfate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
quercetin sulfate biosynthesis
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Flavone and flavonol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:quercetin-3,3'-bissulfate 7-sulfotransferase
Quercetin 3,4'-bissulfate can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
121855-13-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Col0
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate
isorhamnetin 3,7-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
3'-phosphoadenylylsulfate + quercetin 3,3'-bissulfate
quercetin 3,3',7-trissulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
3'-phosphoadenylylsulfate + quercetin 3,4'-bissulfate
quercetin 3,4',7-trissulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
adenosine 3'-phosphate 5'-phosphosulfate + 3-hydroxyflavone
adenosine 3',5'-bisphosphate + 4-oxo-2-phenyl-4H-chromen-3-yl hydrogen sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + 7-hydroxyflavone
adenosine 3',5'-bisphosphate + 7-hydroxyflavone 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + apigenin
adenosine 3',5'-bisphosphate + apigenin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + apigenin 4'-sulfate
adenosine 3',5'-bisphosphate + apigenin 4',7-bissulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + crysin
adenosine 3',5'-bisphosphate + crysin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + dihydroquercetin
adenosine 3',5'-bisphosphate + dihydroquercetin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + galangin
adenosine 3',5'-bisphosphate + galangin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + genistein
adenosine 3',5'-bisphosphate + genistein 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + gossypetin
adenosine 3',5'-bisphosphate + gossypetin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + isorhamnetin
adenosine 3',5'-bisphosphate + isorhamnetin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol
adenosine 3',5'-bisphosphate + kaempferol 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol 3-sulfate
adenosine 3',5'-bisphosphate + kaempferol 3,7-bissulfate
show the reaction diagram
adenosine 3'-phosphate 5'-phosphosulfate + myricetin
adenosine 3',5'-bisphosphate + myricetin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + quercetin
adenosine 3',5'-bisphosphate + quercetin 7-sulfate
show the reaction diagram
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?
adenosine 3'-phosphate 5'-phosphosulfate + rhamnetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + quercetin 3,3'-bissulfate
quercetin 3,3',7-trissulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol 3-sulfate
adenosine 3',5'-bisphosphate + kaempferol 3,7-bissulfate
show the reaction diagram
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no requirement for a divalent cation at 1-10 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Flavonol substrate
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below 0.0015 mM, inhibition
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additional information
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EDTA, SH-group reagents at 1-10 mM, no inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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trans-zeatin induces AtST3a enzyme expression
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00033 - 0.00046
3'-phosphoadenylylsulfate
0.001
adenosine 3'-phosphate 5'-phosphosulfate
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pH 7.5, 25°C, recombinant enzyme
0.002
kaempferol 3-sulfate
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pH 7.5, 25°C, recombinant enzyme
0.0002 - 0.00024
quercetin 3,3'-disulfate
0.00026
Quercetin 3,4'-disulfate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00038
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isoenzyme II
0.00058
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isoenzyme I
0.017
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purified recombinant enzyme, substrate kaempferol 3-sulfate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
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about 40-45% of maximal activity at pH 6.5 and 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 6.8
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chromatofocusing, isoform II
6.5
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chromatofocusing, isoform I
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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root-specific expression of AtST3a in roots of 15-day-old plants
Manually annotated by BRENDA team
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ST3a is expressed at early stages of seedling development
Manually annotated by BRENDA team
additional information
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no expression in leaves of of 15-day-old plants, developmental expression pattern, the AtST3a transcript cannot be detected in extracts of wounded or etiolated plant material
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged AtST3a from Escherichia coli strain XL-1 Blue by nickel affinity and on 3'-phosphoadenosine 5'-phosphate affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
AtST3a, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain XL-1 Blue
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