Information on EC 2.8.2.27 - quercetin-3-sulfate 4'-sulfotransferase

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The expected taxonomic range for this enzyme is: Flaveria chlorifolia

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EC NUMBER
COMMENTARY hide
2.8.2.27
-
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RECOMMENDED NAME
GeneOntology No.
quercetin-3-sulfate 4'-sulfotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + quercetin 3-sulfate = adenosine 3',5'-bisphosphate + quercetin 3,4'-bissulfate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
quercetin sulfate biosynthesis
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-
Flavone and flavonol biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:quercetin-3-sulfate 4'-sulfotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
121855-12-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate
isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
3'-phosphoadenylylsulfate + kaempferol 3-sulfate
kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
3'-phosphoadenylylsulfate + patuletin 3-sulfate
patuletin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
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12% of activity compared to quercetin 3-sulfate
-
?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
additional information
?
-
-
involved in biosynthesis of polysulfated flavonoids
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-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for divalent cations
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Phosphate buffer
-
complete inhibition
-
additional information
-
EDTA up to 10 mM, SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide, all at 1 and 10 mM, are not inhibitory
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00038
3'-phosphoadenylylsulfate
0.00036
quercetin 3-sulfate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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gel filtration
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, bovine serum albumin up to 1 mg/ml, half-life of 3 days
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4°C, half-life of 24 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
construction of chimeric enzymes with EC2.8.2.25 in order to define domains responsible for substrate binding and position
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LINKS TO OTHER DATABASES (specific for EC-Number 2.8.2.27)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)