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Information on EC 2.8.2.21 - keratan sulfotransferase and Organism(s) Mus musculus and UniProt Accession Q9EQC0
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2.8.2.21 keratan sulfotransferaseIUBMB Comments
Sulfation takes place at the 6-position of galactosyl and N-acetylglucosaminyl residues in keratan, a proteoglycan. Not identical with EC 2.8.2.5 (chondroitin 4-sulfotransferase), EC 2.8.2.6 (choline sulfotransferase) or EC 2.8.2.17 (chondroitin 6-sulfotransferase).
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Word Map
- 2.8.2.21
-
sulfotransferases
- l-selectin
- 5'-phosphosulfate
- n-acetyllactosamine
- 3'-phosphoadenosine
-
6-o-sulfation
- glycam-1
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6-sulfated
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n-acetylglucosamine-6-o-sulfotransferase
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retnlb
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addressin
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siglec-f
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
chst1, ksgal6st, chst5, glcnac 6-o-sulfotransferase, glcnac6st, keratan sulfate sulfotransferase, gn6st, keratan sulfate gal-6-sulfotransferase, keratan sulfate galactose 6-o-sulfotransferase, glcnac6st3, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3'-phosphoadenylyl keratan sulfotransferase
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3'-phosphoadenylylsulfate:keratan sulfotransferase
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Gal-6-sulfotransferase
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galactose-6-sulfotransferase
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GlcNAc 6-O-sulfotransferase
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GlcNAc 6-sulfotransferase
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GlcNAc sulfotransferase
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GlcNAc6ST
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Gn6ST
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GST
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GST3
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GST4alpha
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GST4beta
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keratan sulfate Gal-6-sulfotransferase
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keratan sulfate sulfotransferase
KSG6ST
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KSGal6ST
KSST
LSST
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N-acetylgalactosamine 6-O-sulfotransferase
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N-acetylglucosaminyl sulfotransferase
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sulfotransferase, keratan
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keratan sulfate sulfotransferase
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KSGal6ST
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KSST
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sulfate group transfer
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:keratan 6'-sulfotransferase
Sulfation takes place at the 6-position of galactosyl and N-acetylglucosaminyl residues in keratan, a proteoglycan. Not identical with EC 2.8.2.5 (chondroitin 4-sulfotransferase), EC 2.8.2.6 (choline sulfotransferase) or EC 2.8.2.17 (chondroitin 6-sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY
62168-79-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + N-acetyllactosamine
adenosine 3',5'-bisphosphate + 6'-sulfo N-acetyllactosamine
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?
3'-phosphoadenylylsulfate + keratan sulfate
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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sulfation at position 6 of N-acetylgalactosamine residues, GlcNAc 6-O-sulfotransferase
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?
additional information
?
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KSST competes with fucosyltransferase-VII for the same acceptor substrate and downregulates the synthesis of L-selectin ligand by inhibiting alpha1,3-fucosylation, sulfated oligosaccharide, e.g. 6'-sulfated galactose residues, increase in FucT-VII-deficient mutant mice, structure of mono-sulfated O-glycans, detailed overview
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?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
pericellular localization of R-10G reactive KS epitopes in perineuronal net-positive neurons in the visual cortex of adult mice
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + N-acetyllactosamine
adenosine 3',5'-bisphosphate + 6'-sulfo N-acetyllactosamine
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?
additional information
?
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KSST competes with fucosyltransferase-VII for the same acceptor substrate and downregulates the synthesis of L-selectin ligand by inhibiting alpha1,3-fucosylation, sulfated oligosaccharide, e.g. 6'-sulfated galactose residues, increase in FucT-VII-deficient mutant mice, structure of mono-sulfated O-glycans, detailed overview
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-
?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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-
-
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?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
pericellular localization of R-10G reactive KS epitopes in perineuronal net-positive neurons in the visual cortex of adult mice
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-
?
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
enzyme GlcNAc-6-sulfated KS is recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galbeta1-4GlcNAc(6S)beta1-3Galbeta1-4GlcNAc(6S), and distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex
additional information
additional information
immunohistochemic analysis of the enzyme in brain samples
additional information
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immunohistochemic analysis of the enzyme in brain samples
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
the enzyme is essential for C-6 sulfation of galactose within keratin sulfate in early postnatal brains
malfunction
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enzyme knockout mice do not exhibit a detectable deficiency in short-term lymphocyte homing
metabolism
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the enzyme is required for the generation of Siaalpha2->3[6S]beta->4[6S]GlcNAc or a closely related structure in lymph node high endothelial venules. The enzyme does does not contribute to L-selectin ligand expression or lymphocyte homing
physiological function
keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. GlcNAc6ST3 is a major sulfotransferase for the R-10G-positive KS in the adult mouse brain. GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst5 is an enzyme necessary for the synthesis of GlcNAc-6-sulfated KS chains which are required for neuronal plasticity in the visual cortex of the mouse brain during the critical period, but not in adulthood. GlcNAc-6-sulfated KS is recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galbeta1-4GlcNAc(6S)beta1-3Galbeta1-4GlcNAc(6S), and distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex. GlcNAc6ST3, which has been discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood. An isoform of the protein-tyrosine phosphatase PTPRZ is also a R-10G-reactive KS proteoglycan. GlcNAc6ST3 may play a role in synthesis of a component of PNN in the adult brain, and the KS-modified isoform of PTPRZ encoded by Ptprz1 might be an extracellular molecule associated with PNNs
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CHST1_MOUSE
411
1
46904
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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overexpression of KSST suppresses the expression of sialyl Lewis X on CHO cells engineered to express sialyl Lewis X, KSST expression in those cells suppresses lymphocyte rolling compared with mock-transfected CHO cells expressing 6-sulfo sialyl Lewis X, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akama, T.O.; Nakayama, J.; Nishida, K.; Hiraoka, N.; Suzuki, M.; McAuliffe, J.; Hindsgaul, O.; Fukuda, M.; Fukuda, M.N.
Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal GlcNac 6-O-sulfotransferase both produce keratan sulfate
J. Biol. Chem.
276
16271-16278
2001
Homo sapiens, Mus musculus
Hiraoka, N.; Petryniak, B.; Kawashima, H.; Mitoma, J.; Akama, T.O.; Fukuda, M.N.; Lowe, J.B.; Fukuda, M.
Significant decrease in alpha1,3-linked fucose in association with increase in 6-sulfated N-acetylglucosamine in peripheral lymph node addressin of FucT-VII-deficient mice exhibiting diminished lymphocyte homing
Glycobiology
17
277-293
2007
Mus musculus
Patnode, M.L.; Yu, S.Y.; Cheng, C.W.; Ho, M.Y.; Tegesjoe, L.; Sakuma, K.; Uchimura, K.; Khoo, K.H.; Kannagi, R.; Rosen, S.D.
KSGal6ST generates galactose-6-O-sulfate in high endothelial venules but does not contribute to L-selectin-dependent lymphocyte homing
Glycobiology
23
381-394
2013
Mus musculus
Hoshino, H.; Foyez, T.; Ohtake-Niimi, S.; Takeda-Uchimura, Y.; Michikawa, M.; Kadomatsu, K.; Uchimura, K.
KSGal6ST is essential for the 6-sulfation of galactose within keratan sulfate in early postnatal brain
J. Histochem. Cytochem.
62
145-156
2014
Mus musculus (Q9EQC0)
Narentuya, ; Takeda-Uchimura, Y.; Foyez, T.; Zhang, Z.; Akama, T.O.; Yagi, H.; Kato, K.; Komatsu, Y.; Kadomatsu, K.; Uchimura, K.
GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
Sci. Rep.
9
4387
2019
Mus musculus (Q9QUP4), Mus musculus, Mus musculus C57BL/6 J (Q9QUP4)
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