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Information on EC 2.8.2.20 - protein-tyrosine sulfotransferase and Organism(s) Mus musculus and UniProt Accession O70281

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     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.20 protein-tyrosine sulfotransferase
IUBMB Comments
The tyrosine residues of some specific proteins of rat pheochromocytoma cells act as acceptors.
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Select one or more organisms in this record:
This record set is specific for:
Mus musculus
UNIPROT: O70281
Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
AQC1, sulfotransferase, protein (tyrosine), TPST, TPST-1, TPST-2, TPST-A, TPST1, TPST2, tyrosylprotein sulfotransferase, tyrosylprotein sulfotransferase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfotransferase, protein (tyrosine)
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-
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TPST-1
244
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TPST-2
244
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TPST1
244
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tyrosylprotein sulfotransferase
tyrosylprotein sulfotransferase-1
244
-
tyrosylprotein sulfotransferase-2
244
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:protein-tyrosine O-sulfotransferase
The tyrosine residues of some specific proteins of rat pheochromocytoma cells act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
87588-33-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + CCR8 1-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 1-17 M1A peptide
show the reaction diagram
-
substrate is a synthetic modified mouse CCR8 peptide ADYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr3 is sulfated first, followed by Tyr14, with Tyr15 last
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-
?
3'-phosphoadenylyl sulfate + CCR8 2-17 M1A peptide
adenosine 3',5'-bisphosphate + sulfated CCR8 2-17 M1A peptide
show the reaction diagram
-
substrate is a synthetic modified mouse CCR8 peptide DYTMEPNVTMTDYYPD-NH2. Sulfation pattern: Tyr14 is sulfated first, followed by Tyr3 and Tyr15
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-
?
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + acidic amino acid polymer EAY
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
i.e. poly-EAY or Glu6,Ala3,Tyr1, model substrate
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-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
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-
?
3'-phosphoadenylylsulfate + QATEYEYLDYDFLPEC
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
derived from N-terminal residues of the mature leukocyte adhesion molecule P-selectin glycoprotein ligand 1
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + Mfge8 protein tyrosine
adenosine 3',5'-bisphosphate + Mfge8 protein tyrosine O-sulfate
show the reaction diagram
-
the long isoform of Mfge8, substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
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-
?
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + protein tyrosine
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
show the reaction diagram
-
tyrosine sulfonation of TSHR by TPST is crucial for thyrotropin signaling, overview
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-
?
3'-phosphoadenylyl sulfate + RNase 9 protein tyrosine
adenosine 3',5'-bisphosphate + RNase 9 protein tyrosine O-sulfate
show the reaction diagram
-
substrate of isozyme TPST-2, not isozyme TPST-1, mutational analysis, overview
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-
?
3'-phosphoadenylyl sulfate + TSH receptor
adenosine 3',5'-bisphosphate + TSH receptor O-sulfate
show the reaction diagram
-
tyrosine sulfonation of TSHR by TPST2 is crucial for thyrotropin signaling, TPST1 and TPST2 mutant H266Q are incapable of mediating the signal transduction, overview
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-
?
3'-phosphoadenylylsulfate + protein tyrosine
adenosine 3',5'-bisphosphate + acidic polypeptide tyrosine-O-sulfate
show the reaction diagram
-
post-translational modification of biologically active peptides and proteins
-
-
?
additional information
?
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
development of a specific detection method using an anti-sulfotyrosine monoclonal antibody, termed PSG2, that binds with high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independently of sequence context, overview
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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cauda
Manually annotated by BRENDA team
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primary
Manually annotated by BRENDA team
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low TPST-2 expression level
Manually annotated by BRENDA team
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isozymes TPST-1 and TPST-2
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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deficiency in the enzymatic activity of isozyme TPST2 causes growth-retardation in mice, autosomal recessive hypothyroidism, and lifelong female infertility. The isozyme deficiency is also involved in severe thyroid hypogenesis and consequent dwarfism, due to the impairment of the tyrosine sulfation of thyroid-stimulating hormone receptor by mutant TPST2
metabolism
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the two independent TPSTs catalyze the tyrosine-O-sulfation, a post-translational modification
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
TPST1_MOUSE
370
0
42132
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42130
calculated from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
epitope mapping
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H266Q
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missense mutation of Tpst2, devoid of activity, in the highly conserved region of the gene leads to the autosomal recessive, fetal-onset, severe thyroid hypoplasia phenotype related to TSH hyporesponsiveness in growth-retarded mice, the mutant enzyme shows unaltered subcellular localization, overview
additional information
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
develoment of an immunoaffinity purification method using anti-sulfotyrosine monoclonal antibody PSG2, recombinantly expressed in CHO cells
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
gene Tpst2, wild-type and mutant enzymes genotyping and genetic mapping, expression of genes Tpst1 and Tpst2 in grt mouse cells, rescue of the phenotype, overview
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isozyme TPST-2, expression analysis
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isozymes TPST-1 and TPSt-2, geno- and phenotyping of wild-types and mutants
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isozymes TPST-1 and TPST-2, genotyping
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isozymes TPST-1 and TPST-2. TPST-1 DNA and amino acid sequence determinationand analysis, sequence comparison
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rens-Domiano, S.; Roth, J.A.
Characterization of tyrosylprotein sulfotransferase from rat liver and other tissues
J. Biol. Chem.
264
899-905
1989
Homo sapiens, Mesocricetus auratus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Manually annotated by BRENDA team
Ouyang, Y.; Lane, W.S.; Moore, K.L.
Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins
Proc. Natl. Acad. Sci. USA
95
2896-2901
1998
Homo sapiens, Homo sapiens (O60507), Mus musculus, Mus musculus (O70281), Rattus norvegicus
Manually annotated by BRENDA team
Hoffhines, A.J.; Damoc, E.; Bridges, K.G.; Leary, J.A.; Moore, K.L.
Detection and purification of tyrosine-sulfated proteins using a novel anti-sulfotyrosine monoclonal antibody
J. Biol. Chem.
281
37877-37887
2006
Mus musculus
Manually annotated by BRENDA team
Borghei, A.; Ouyang, Y.B.; Westmuckett, A.D.; Marcello, M.R.; Landel, C.P.; Evans, J.P.; Moore, K.L.
Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility
J. Biol. Chem.
281
9423-9431
2006
Mus musculus
Manually annotated by BRENDA team
Sasaki, N.; Hosoda, Y.; Nagata, A.; Ding, M.; Cheng, J.M.; Miyamoto, T.; Okano, S.; Asano, A.; Miyoshi, I.; Agui, T.
A mutation in Tpst2 encoding tyrosylprotein sulfotransferase causes dwarfism associated with hypothyroidism
Mol. Endocrinol.
21
1713-1721
2007
Mus musculus
Manually annotated by BRENDA team
Kanan, Y.; Hoffhines, A.; Rauhauser, A.; Murray, A.; Al-Ubaidi, M.R.
Protein tyrosine-O-sulfation in the retina
Exp. Eye Res.
89
559-567
2009
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Westmuckett, A.D.; Hoffhines, A.J.; Borghei, A.; Moore, K.L.
Early postnatal pulmonary failure and primary hypothyroidism in mice with combined TPST-1 and TPST-2 deficiency
Gen. Comp. Endocrinol.
156
145-153
2008
Mus musculus
Manually annotated by BRENDA team
Hoffhines, A.J.; Jen, C.H.; Leary, J.A.; Moore, K.L.
Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo
J. Biol. Chem.
284
3096-3105
2009
Mus musculus
Manually annotated by BRENDA team
Hosoda, Y.; Sasaki, N.; Agui, T.
Female infertility in grt mice is caused by thyroid hormone deficiency, not by insufficient TPST2 activity in the reproductive organs
J. Vet. Med. Sci.
70
1043-1049
2008
Mus musculus
Manually annotated by BRENDA team
Stone, M.J.; Chuang, S.; Hou, X.; Shoham, M.; Zhu, J.Z.
Tyrosine sulfation: an increasingly recognised post-translational modification of secreted proteins
N. Biotechnol.
25
299-317
2009
Bos taurus, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
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