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Information on EC 2.8.2.2 - alcohol sulfotransferase and Organism(s) Rattus norvegicus and UniProt Accession P22789

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.2 alcohol sulfotransferase
IUBMB Comments
Primary and secondary alcohols, including aliphatic alcohols, ascorbic acid, chloramphenicol, ephedrine and hydroxysteroids, but not phenolic steroids, can act as acceptors (cf. EC 2.8.2.15 steroid sulfotransferase).
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Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: P22789
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
hydroxysteroid, estrogen sulfotransferase, dhea st, sult2b1a, sult6b1, iodothyronine sulfotransferase, ast-rb2, pz-sult, hydroxysteroid sulfotransferase sult2a1, hydroxysteroid sulfotransferase 2b1b, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxysteroid sulfotransferase
-
-
-
-
3beta-hydroxy steroid sulfotransferase
-
-
-
-
3beta-hydroxysteroid sulfotransferase
-
-
-
-
5alpha-androstenol sulfotransferase
-
-
-
-
alcohol sulfotransferase
-
-
alcohol/hydroxysteroid sulfotransferase
-
-
-
-
cholesterol sulfotransferase
-
-
-
-
dehydroepiandrosterone sulfotransferase
DELTA5-3beta-hydroxysteroid sulfokinase
-
-
-
-
estrogen sulfokinase
-
-
-
-
estrogen sulfotransferase
-
-
-
-
HST
-
-
-
-
hydroxysteroid sulfotransferase
-
-
-
-
hydroxysteroid sulfotransferase 1
-
-
hydroxysteroid sulfotransferase Sta
-
-
phenol sulfotransferase
-
-
steroid alcohol sulfotransferase
-
-
-
-
steroid sulfokinase
-
-
-
-
steroid sulfotransferase
-
-
-
-
steroid/sterol sulfotransferase
-
sterol sulfokinase
-
-
-
-
sterol sulfotransferase
-
-
-
-
additional information
the enzyme belongs to the SULT2 family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:alcohol sulfotransferase
Primary and secondary alcohols, including aliphatic alcohols, ascorbic acid, chloramphenicol, ephedrine and hydroxysteroids, but not phenolic steroids, can act as acceptors (cf. EC 2.8.2.15 steroid sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9032-76-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + (R)-(+)-1-phenyl-1-heptanol
adenosine 3',5'-bisphosphate + (R)-(+)-1-phenyl-1-heptyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + (S)-(-)-1-phenyl-1-heptanol
adenosine 3',5'-bisphosphate + (S)-(-)-1-phenyl-1-heptyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + 7-hydroxymethyl-12-methylbenz[a]anthracene sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + R-(+)-1-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 3beta,20beta-pregnenediol
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + an alcohol
adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + (R)-(-)-2-heptanol
adenosine 3',5'-bisphosphate + (R)-(-)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(+)-2-heptanol
adenosine 3',5'-bisphosphate + (S)-(+)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-butanol
adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 1-decanol
adenosine 3',5'-bisphosphate + 1-decyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-dodecanol
adenosine 3',5'-bisphosphate + 1-dodecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-heptanol
adenosine 3',5'-bisphosphate + 1-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hexanol
adenosine 3',5'-bisphosphate + 1-hexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-methylcyclohexanol
adenosine 3',5'-bisphosphate + 1-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-nonanol
adenosine 3',5'-bisphosphate + 1-nonyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-octanol
adenosine 3',5'-bisphosphate + 1-octyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-pentanol
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-propanol
adenosine 3',5'-bisphosphate + 1-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-tetradecanol
adenosine 3',5'-bisphosphate + 1-tetradecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-undecanol
adenosine 3',5'-bisphosphate + 1-undecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 2-methyl-2-hexanol
adenosine 3',5'-bisphosphate + 1,1-dimethylpentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-propanol
adenosine 3',5'-bisphosphate + 2-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-ethyl-3-pentanol
adenosine 3',5'-bisphosphate + 3-ethyl-3-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-methyl-1-butanol
adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 5%
-
-
?
3'-phosphoadenylylsulfate + 3beta,17alpha-dihydroxy-pregn-5-ene-20-one
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + 3beta-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 45%
-
-
r
3'-phosphoadenylylsulfate + 4-butylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-butylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-ethylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-ethylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptanol
adenosine 3',5'-bisphosphate + 4-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-heptylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hexylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-hexylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-methylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-methylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-octylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-octylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-pentylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-pentylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-propylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-propylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 20%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3beta,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 83%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-pregnane-3beta,20alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 86%
-
-
r
3'-phosphoadenylylsulfate + 5beta-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 7%
-
-
r
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
carcinogen
-
-
?
3'-phosphoadenylylsulfate + aldosterone
adenosine 3',5'-bisphosphate + aldosterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + alpha-naphthol
adenosine 3',5'-bisphosphate + alpha-naphthyl sulfate
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + amyl alcohol
adenosine 3',5'-bisphosphate + amyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17beta-diol
adenosine 3',5'-bisphosphate + androst-5-en-3beta,17beta-diol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ascorbic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + benzyl alcohol
adenosine 3',5'-bisphosphate + benzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + chloramphenicol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cis-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + cis-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + corticosterone
adenosine 3',5'-bisphosphate + corticosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cortisone
adenosine 3',5'-bisphosphate + cortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cyclohexylmethanol
adenosine 3',5'-bisphosphate + cyclohexylmethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + D-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + E-(+)-alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
E-(+)-alpha-hydroxytamoxifen is the only substrate, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen inhibit sulfation of E-(+)-alpha-hydroxytamoxifen
-
-
?
3'-phosphoadenylylsulfate + ephedrine
adenosine 3',5'-bisphosphate + ephedrine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + epiandrosterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
3beta-hydroxy-5alpha-androstan-17-one
-
-
r
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol sulfate
show the reaction diagram
-
poor substrate, relative activity 8% with beta-estradiol as 100%
-
-
r
3'-phosphoadenylylsulfate + ethanol
adenosine 3',5'-bisphosphate + ethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + hydrocortisone
adenosine 3',5'-bisphosphate + hydrocortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + isoamyl alcohol
adenosine 3',5'-bisphosphate + isoamyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-tyrosinemethyl ester
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 9%
-
-
r
3'-phosphoadenylylsulfate + methanol
adenosine 3',5'-bisphosphate + methyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + ouabain
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol
adenosine 3',5'-bisphosphate + (3alpha,20S)-20-hydroxypregn-5-en-3-yl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + retinol
adenosine 3',5'-bisphosphate + retinol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + trans-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + trans-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + tridecan-1-ol
adenosine 3',5'-bisphosphate + tridecyl sulfate
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylyl sulfate + an alcohol
adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
crude enzyme activity is markedly stimulated by divalent cations like Fe2+, Co2+ and Mn2+, this effect is not observed with purified enzyme
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dehydroepiandrosterone
substrate inhibition
S-(-)-1-naphthyl-1-ethanol
-
adenosine 3',5'-bisphosphate
E-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
p-chloromercuribenzoate
-
-
Z-(+)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
Z-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
-
activity of the purified enzyme is slightly enhanced
additional information
-
stimulated by nonionic detergents
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.96
(R)-(+)-1-naphthyl-1-ethanol
pH 7.0, 37°C
0.15
(R)-(+)-1-phenyl-1-heptanol
pH 7.0, 37°C
0.31
(S)-(-)-1-phenyl-1-heptanol
pH 7.0, 37°C
0.72
7-hydroxymethyl-12-methylbenz[a]anthracene
pH 7.0, 37°C
0.12
(12-methyltetraphen-7-yl)methanol
-
pH 5.5, 37°C
4.7
(R)-(-)-2-heptanol
-
pH 5.5, 37°C
4.9
(S)-(+)-2-heptanol
-
pH 5.5, 37°C
1.7
1-amylalcohol
-
pH 5.5, 37°C
3 - 106
1-butanol
0.31
1-Decanol
-
pH 5.5, 37°C
0.33
1-Dodecanol
-
pH 5.5, 37°C
2.2
1-Heptanol
-
pH 5.5, 37°C
4.3
1-Hexanol
-
pH 5.5, 37°C
26.8
1-methylcyclohexanol
-
pH 5.5, 37°C
0.37
1-nonanol
-
pH 5.5, 37°C
0.77
1-Octanol
-
pH 5.5, 37°C
1.7 - 19.2
1-Pentanol
24 - 610
1-propanol
0.23
1-Tetradecanol
-
pH 5.5, 37°C
0.23
1-tridecanol
-
pH 5.5, 37°C
0.26
1-undecanol
-
pH 5.5, 37°C
0.52
11-deoxycorticosterone
-
pH 7.5, 37°C
24.6
2-methyl-2-hexanol
-
pH 5.5, 37°C
17
2-propanol
-
pH 7.5, 37°C
0.012 - 0.1
3'-phosphoadenylylsulfate
40.3
3-ethyl-3-pentanol
-
pH 5.5, 37°C
1.2
3-methyl-1-butanol
-
pH 7.5, 37°C
0.00063
3beta,20beta-pregnenediol
-
pH 7.5, 37°C
0.07 - 0.16
4-butylbenzyl alcohol
0.67
4-Ethylbenzyl alcohol
-
pH 5.5, 37°C
7.2
4-heptanol
-
pH 5.5, 37°C
0.19
4-heptylbenzyl alcohol
-
pH 5.5, 37°C
0.2
4-hexylbenzyl alcohol
-
pH 5.5, 37°C
0.07 - 2.19
4-methylbenzyl alcohol
0.29
4-octylbenzyl alcohol
-
pH 5.5, 37°C
0.12
4-pentylbenzyl alcohol
-
pH 5.5, 37°C
0.4
4-propylbenzyl alcohol
-
pH 5.5, 37°C
0.17
aldosterone
-
pH 7.5, 37°C
25.8
benzyl alcohol
-
pH 5.5, 37°C
0.035 - 0.29
beta-estradiol
0.0017 - 0.0025
cholesterol
4.5
cis-4-methylcyclohexanol
-
pH 5.5, 37°C
0.29 - 0.44
cortisol
2
cyclohexylmethanol
-
pH 5.5, 37°C
0.006 - 0.0179
dehydroepiandrosterone
0.35
estriol
-
pH 7.5, 37°C
42 - 47
ethanol
1
Hexanol
-
pH 7.5, 37°C
1.2
Isoamylalcohol
-
pH 5.5, 37°C
0.0016 - 0.0089
pregnenolone
0.07
testosterone
5.1
trans-4-methylcyclohexanol
-
pH 5.5, 37°C
additional information
additional information
steady-state kinetic analysis of recombinant isozymes SULT2B1a and SULT2B1b
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.283
1-butanol
-
pH 7.5, 37°C
0.417
1-Hexanol
-
pH 7.5, 37°C
0.45
1-Pentanol
-
pH 7.5, 37°C
0.183
1-propanol
-
pH 7.5, 37°C
0.183
11-deoxycorticosterone
-
pH 7.5, 37°C
0.217
2-propanol
-
pH 7.5, 37°C
0.417
3-methyl-1-butanol
-
pH 7.5, 37°C
2
3beta-hydroxyandrost-5-ene-17-one
-
pH 7.5, 37°C
0.133
aldosterone
-
pH 7.5, 37°C
0.583
beta-estradiol
-
pH 7.5, 37°C
0.183
cortisol
-
pH 7.5, 37°C
0.15
cortisone
-
pH 7.5, 37°C cosubstrate ethanol
0.167
estriol
-
pH 7.5, 37°C
0.0833
methanol
-
pH 7.5, 37°C
0.467
testosterone
-
pH 7.5, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
(S)-(-)-1-naphthyl-1-ethanol
pH 5.5, 37°C
0.003
adenosine 3',5'-bisphosphate
-
pH 8.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.11
-
substrate estradiol
0.15
-
substrate n-butanol
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
-
beta-estradiol and dehydroepiandrosterone as substrate
6
-
dehydroepiandrosterone as substrate
8
-
broad pH optimum centered on pH 8.0
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.12
sequence calculation, SULT2B1b
5.93
sequence calculation, SULT2B1a
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
epidermal keratinocyte
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ST2A2_RAT
284
0
33252
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
180000
28000
-
6 * 28000, SDS-PAGE
30200
-
SDS-PAGE
38319
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation
40000
-
SDS-PAGE
42178
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation
50000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation
hexamer
-
6 * 28000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
unstable at low pH
645773
6 - 9
-
stable for 30 min at 37°C
645773
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
partially purified enzyme loses 30% of upon freeze-thawing, activity can be stabilized by adenosine 3',5'-bisphosphate
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, activity towards dehydroepiandrosterone as acceptor substrate is stable for several months
-
-20°C, partially purified enzyme in 50% glycerol or ethylene glycol loses 30% of activity overnight
-
-80°C, stable for several months
-
4°C, activity declines by 60% after 4 weeks
-
4°C, loses 10% activity per week upon storage
-
4°C, partially purified enzyme loses 30% of activity overnight
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native and recombinant protein
recombinant GST-tagged isozymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
sulfotransferase 1
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
STa cloned into pET-3c vector and expressed in Escherichia coli BL21
gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, comparison of sequence and genetic structure, overview, overexpression of GST-tagged isozymes in Escherichia coli strain BL21(DE3)
SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
a 2 week's ethanol ingestion up-regulates phenol sulfotransferase SULT1A1 in male rat liver and intestine
-
after different doses of dopamine (0, 2, 10 and 100 mg/kg/day) are administrated to both male and female rats for 7 days, the activity, protein level and mRNA expression of the recombinant enzyme SULT2A1 increases significantly
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
model studies on the carcinogenesis of benzylic and allylic alcohols
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ryan, R.A.; Carroll, J.
Studies on a 3beta-hydroxysteroid sulphotransferase from rat liver
Biochim. Biophys. Acta
429
391-401
1976
Rattus norvegicus
Manually annotated by BRENDA team
Lyon, E.S.; Jakoby, W.B.
The identity of alcohol sulfotransferases with hydroxysteroid sulfotransferases
Arch. Biochem. Biophys.
202
474-481
1980
Rattus norvegicus
Manually annotated by BRENDA team
Lyon, E.S.; Marcus, C.J.; Wang, J.L.; Jakoby, W.B.
Hydroxysteroid sulfotransferase
Methods Enzymol.
77
206-213
1981
Rattus norvegicus
Manually annotated by BRENDA team
Chen, G.; Banoglu, E.; Duffel, M.W.
Influence of substrate structure on the catalytic efficiency of hydroxysteroid sulfotransferase STa in the sulfation of alcohols
Chem. Res. Toxicol.
9
67-74
1996
Rattus norvegicus
Manually annotated by BRENDA team
Strott, C.A.
Steroid sulfotransferases
Endocr. Rev.
17
670-697
1996
Cavia porcellus, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Rearick, J.I.; Calhoun, E.S.
Purification and characterization of cholesterol sulfotransferase from rat skin
Biochem. Cell Biol.
79
499-506
2001
Rattus norvegicus
Manually annotated by BRENDA team
Sheng, J.J.; Duffel, M.W.
Bacterial expression, purification, and characterization of rat hydroxysteroid sulfotransferase STa
Protein Expr. Purif.
21
235-242
2001
Rattus norvegicus (P22789)
Manually annotated by BRENDA team
Chen, W.T.; Liu, M.C.; Yang, Y.S.
Fluorometric assay for alcohol sulfotransferase
Anal. Biochem.
339
54-60
2005
Escherichia coli, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Apak, T.I.; Duffel, M.W.
Interactions of the stereoisomers of alpha-hydroxytamoxifen with human hydroxysteroid sulfotransferase SULT2A1 and rat hydroxysteroid sulfotransferase STa
Drug Metab. Dispos.
32
1501-1508
2004
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Kohjitani, A.; Fuda, H.; Hanyu, O.; Strott, C.A.
Cloning, characterization and tissue expression of rat SULT2B1a and SULT2B1b steroid/sterol sulfotransferase isoforms: Divergence of the rat SULT2B1 gene structure from orthologous human and mouse genes
Gene
367
66-73
2005
Rattus norvegicus (Q29YR5)
Manually annotated by BRENDA team
Falany, C.N.; He, D.; Dumas, N.; Frost, A.R.; Falany, J.L.
Human cytosolic sulfotransferase 2B1: isoform expression, tissue specificity and subcellular localization
J. Steroid Biochem. Mol. Biol.
102
214-221
2006
Homo sapiens (O00204), Homo sapiens, Mus musculus (O35400), Rattus norvegicus (Q29YR5)
Manually annotated by BRENDA team
Maiti, S.; Chen, G.
Ethanol up-regulates phenol sulfotransferase (SULT1A1) and hydroxysteroid sulfotransferase (SULT2A1) in rat liver and intestine
Arch. Physiol. Biochem.
121
68-74
2015
Rattus norvegicus
Manually annotated by BRENDA team
Shao, X.; Li, J.; Wang, S.; Chen, G.; Xu, J.; Ji, X.; Li, L.; Lu, W.; Zhou, T.
Exogenous dopamine induces dehydroepiandrosterone sulfotransferase (rSULT2A1) in rat liver and changes the pharmacokinetic profile of moxifloxacin in rats
Drug Metab. Pharmacokinet.
30
97-104
2015
Rattus norvegicus
Manually annotated by BRENDA team