Information on EC 2.8.2.18 - cortisol sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.18
-
RECOMMENDED NAME
GeneOntology No.
cortisol sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + cortisol = adenosine 3',5'-bisphosphate + cortisol 21-sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:cortisol 21-sulfotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
71427-08-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
guinea pig, male and female albinos, Hartley strain
-
-
Manually annotated by BRENDA team
Meriones sp.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol 21-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + deoxycorticosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estradiol-17beta
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol 21-sulfate
show the reaction diagram
additional information
?
-
-
involved in control of corticosteroid metabolism
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3'-phosphoadenylylsulfate
-
inhibition above 0.33 mM
adenosine 3',5'-bisphosphate
-
product inhibition
Antibodies to sulfotransferase III
-
sulfotransferase I
-
Cd2+
-
5 mM, strong inhibition
corticosterone
-
cortisol as substrate, strong
cortisol
-
inhibition above 0.08 mM
Cortisol 21-sulfate
-
product inhibition
cortisone
-
cortisol as substrate, weak, 0.012 mM
dehydroepiandrosterone
deoxycorticosterone
-
cortisol as substrate, strong
dexamethasone
diethylstilbestrol
estradiol-17beta
progesterone
testosterone
-
cortisol as substrate, strong
tetrahydrocortisol
Zn2+
-
5 mM, strong inhibition
additional information
-
no inhibition by adenine nucleotides
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no activation by adenine nucleotides
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00628 - 0.188
3'-phosphoadenylylsulfate
0.00648 - 0.087
cortisol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.012
-
sulfotransferase I
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
cortisol as substrate
6.3
Meriones sp.
-
-
6.5
-
sulfotransferase I
6.7 - 6.9
-
-
7.4
-
sulfotransferase II
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3 - 6.8
-
about half-maximal activity at pH 5.3 and pH 6.8
6 - 8.4
-
about half-maximal activity at pH 6 and pH 8.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
tissue distribution
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
6 * 28000, sulfotransferase I, SDS-PAGE
30000
-
2 * 30000, sulfotransferase III, SDS-PAGE
45700
-
gel filtration, 2 enzyme species: 62100 Da and 45700 Da
61500
-
sulfotransferase III, gel filtration
62100
-
gel filtration, 2 enzyme species: 62100 Da and 45700 Da
65000
-
sedimentation velocity experiments
68000
-
gel filtration
98300
Meriones sp.
-
gel filtration
105000
-
gel filtration
156000
-
sulfotransferase I, sedimentation velocity experiments
160000
-
sulfotransferase I
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 30000, sulfotransferase III, SDS-PAGE
hexamer
-
6 * 28000, sulfotransferase I, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 3 months
-
-20°C, at least 3 weeks
-
-20°C, partially purified preparation, 1 week
-
0-4°C, in crude extracts, 3 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, 2 sulfotransferases: I and II
-
partial; sulfotransferase I, restricted to female rats
-
partial; sulfotransferase III
-
sulfotransferase I; the use of frozen liver or cytosol stored on ice for more than 1-2 h reduces the yield of sulfotransferase I greatly
-
sulfotransferase III
-