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Information on EC 2.8.2.17 - chondroitin 6-sulfotransferase and Organism(s) Gallus gallus and UniProt Accession Q92179

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.17 chondroitin 6-sulfotransferase
IUBMB Comments
The sulfation is at the 6-position of N-acetylgalactosamine residues of chondroitin. Not identical with EC 2.8.2.5 chondroitin 4-sulfotransferase.
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This record set is specific for:
Gallus gallus
UNIPROT: Q92179
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The taxonomic range for the selected organisms is: Gallus gallus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
chst3, c6st, c6st-1, chondroitin 6-sulfotransferase, carbohydrate sulfotransferase 3, c6st1, chondroitin 6-o-sulfotransferase, chondroitin 6-o-sulfotransferase-1, chondroitin-6-sulfotransferase, chondroitin 6-sulfotransferase-1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'-phosphoadenosine 5'-phosphosulfate:chondroitin sulfate sulfotransferase
-
-
-
-
C6ST
-
-
-
-
chondroitin 6-O-sulfotransferase
-
-
-
-
CST
-
-
-
-
sulfotransferase, chondroitin 6-
-
-
-
-
terminal 6-sulfotransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 6'-sulfotransferase
The sulfation is at the 6-position of N-acetylgalactosamine residues of chondroitin. Not identical with EC 2.8.2.5 chondroitin 4-sulfotransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
37292-93-6
-
83589-04-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
involved in biosynthesis of chondroitin sulfate
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Ca2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Co2+
-
good activator, stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Sr2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Zn2+
-
can partially replace Mn2+ in activation
additional information
-
Ca2+ has no effect
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
in absence of added metal ion
additional information
-
no inhibition by detergents of enzyme in chicken cartilage system
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Basic proteins
-
stimulate
-
histone
-
stimulates, optimum concentration: 0.5 mg/ml
lysozyme
-
stimulates, optimum concentration: 3.0 mg/ml
-
polyamines
-
stimulate
-
protamine
spermidine
-
stimulates, optimum concentration: 2.5 mM
spermine
-
stimulates, optimum concentration: 0.6 mM
additional information
-
enzyme is present in supernatant and in particulate fraction, detergent activates indirectly by releasing enzyme into the medium
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007 - 0.0333
3'-phosphoadenylylsulfate
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
-
-
5.8
-
chondroitin 6-sulfotransferase activity
6.2
-
keratan sulfate sulfotransferase activity
6.8
-
imidazole buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
pH 4.0: about 30% of activity maximum, pH 9.0: about 55% of activity maximum
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
embryo chondrocytes release enzyme into the cell culture medium
Manually annotated by BRENDA team
-
cartilage, embryo
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHST3_CHICK
458
1
52253
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
glycolysated enzyme, SDS-PAGE
150000
-
gel filtration
160000
-
gel filtration
75000
-
2 * 75000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 75000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
N-linked glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
thermal inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homogeneity
-
partial
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Habuchi, O.; Miyashita, N.
Separation and characterization of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase from chick embryo cartilage
Biochim. Biophys. Acta
717
414-421
1982
Gallus gallus
Manually annotated by BRENDA team
Sugumaran, G.; Silbert, J.E.
Sulfation of chondroitin. Specificity, degree of sulfation, and detergent effects with 4-sulfating and 6-sulfating microsomal systems
J. Biol. Chem.
263
4673-4678
1988
Gallus gallus
Manually annotated by BRENDA team
Sugumaran, G.; Cogburn, J.N.; Silbert, J.E.
Simultaneous sulfation of endogenous chondroitin sulfate and chondroitin-derived oligosaccharides. Studies with separate 4-sulfating and 6-sulfating microsomal systems
J. Biol. Chem.
261
12659-12664
1986
Gallus gallus
Manually annotated by BRENDA team
Habuchi, O.; Tsuzuki, M.; Takeuchi, I.; Hara, M.; Matsui, Y.; Ashikari, S.
Secretion of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase from cultured chick embryo chondrocytes
Biochim. Biophys. Acta
1133
9-16
1991
Gallus gallus
Manually annotated by BRENDA team
Delfert, D.M.; Conrad, H.E.
Sulfation of chondroitin oligosaccharides in vitro. Analysis of sulfation ratios
J. Biol. Chem.
260
14446-14451
1985
Gallus gallus
Manually annotated by BRENDA team
Salac, M.L.B.; Mourao, P.A.S.
Effects of detergents on the sulfation of chondroitin sulfate by sulfotransferase from chicken embryo epiphyseal cartilage
Biochim. Biophys. Acta
1074
130-135
1991
Gallus gallus
Manually annotated by BRENDA team
Habuchi, O.; Matsui, Y.; Kotoya, Y.; Aoyama, Y.; Yasuda, Y.; Noda, M.
Purification of chondroitin 6-sulfotransferase secreted from cultured chick embryo chondrocytes
J. Biol. Chem.
268
21968-21974
1993
Gallus gallus
Manually annotated by BRENDA team
Salac, M.L.B.; Santos, J.A.; Mourao, P.A.S.
Protamine increases the affinity of 3-phosphoadenosine 5-phosphosulfate toward a sulfotransferase from chicken embryo epiphyseal cartilage
Biochim. Biophys. Acta
883
605-609
1986
Gallus gallus
Manually annotated by BRENDA team
Fukuta, M.; Uchimura, K.; Nakashima, K.; Kato, M.; Kimata, K.; Shinomura, T.; Habuchi, O.
Molecular cloning and expression of chick chondrocyte chondroitin 6-sulfotransferase
J. Biol. Chem.
270
18575-18580
1995
Gallus gallus (Q92179)
Manually annotated by BRENDA team
Habuchi, O.; Hirahara, Y.; Uchimura, K.; Fukuta, M.
Enzymatic sulfation of galactose residue of keratan sulfate by chondroitin 6-sulfotransferase
Glycobiology
6
51-57
1996
Gallus gallus
Manually annotated by BRENDA team
Habuchi, O.; Suzuki, Y.; Fukuta, M.
Sulfation of sialyl lactosamine oligosaccharides by chondroitin 6-sulfotransferase
Glycobiology
7
405-412
1997
Gallus gallus
Manually annotated by BRENDA team