Information on EC 2.8.2.16 - thiol sulfotransferase

for references in articles please use BRENDA:EC2.8.2.16
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.8.2.16
-
RECOMMENDED NAME
GeneOntology No.
thiol sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + a thiol = adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:thiol S-sulfotransferase
Also acts on dithiols; substrates include glutathione, dithioerythritol and 2,3-mercaptopropanol.
CAS REGISTRY NUMBER
COMMENTARY hide
70356-45-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. Samsun
-
-
Manually annotated by BRENDA team
cyanobacterium
-
-
Manually annotated by BRENDA team
cyanobacterium
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + 2',3'-dimercaptopropanol
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + a thiol
adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cysteine
adenosine 3',5'-bisphosphate + 2-amino-3-sulfosulfanyl-propionic acid
show the reaction diagram
-
at about 25% the rate with dithiols
-
-
?
3'-phosphoadenylylsulfate + dithioerythritol
?
show the reaction diagram
3'-phosphoadenylylsulfate + glutathione
?
show the reaction diagram
-
at about 25% the rate with dithiols
-
-
?
3'-phosphoadenylylsulfate + mercaptoethanol
adenosine 3',5'-bisphosphate + thiosulfuric acid S-ethylester
show the reaction diagram
-
at about 25% the rate with dithiols
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + a thiol
adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thioredoxin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
20% activation at 5-10 mM, severe inhibition above 50 mM
Na2SO4
-
required, activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',5'-ADP
3',5'-ADP
5'-ADP
5'-AMP
adenosine 5'-phosphosulfate
-
i.e. APS, strong, reversible by higher PAPS-concentrations
Mg2+
-
above 50 mM, activation at 5-10 mM
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0083 - 0.02
3'-phosphoadenylylsulfate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
5'-ADP
-
pH 8.5
0.43
5'-AMP
-
pH 8.5
0.3
adenosine 5'-phosphosulfate
-
pH 8.5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
about 60% of maximal activity at pH 7.0 and about half-maximal activity at pH 10.0
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial