Information on EC 2.8.2.14 - bile-salt sulfotransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.8.2.14
-
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RECOMMENDED NAME
GeneOntology No.
bile-salt sulfotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
-
-
-
-
3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate
show the reaction diagram
both conjugated and unconjugated bile salts can act as acceptors
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolism of xenobiotics by cytochrome P450
-
-
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:glycolithocholate sulfotransferase
The formation of sulfate esters of bile acids is an essential step in the prevention of toxicity by monohydroxy bile acids in many species [3]. This enzyme is both a bile salt and a 3-hydroxysteroid sulfotransferase. In addition to the 5beta-bile acid glycolithocholate, deoxycholate, 3beta-hydroxy-5-cholenoate and dehydroepiandrosterone (3beta-hydroxyandrost-5-en-17-one) also act as substrates [see also EC 2.8.2.2 (alcohol sulfotransferase) and EC 2.8.2.34 (glycochenodeoxycholate sulfotransferase)]. May be identical to EC 2.8.2.2 [3].
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CAS REGISTRY NUMBER
COMMENTARY hide
65802-92-8
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
guinea pig
-
-
Manually annotated by BRENDA team
rhesus monkey
-
-
Manually annotated by BRENDA team
aging does not influence the response to the hormonal and xenobiotic signaling for enzyme Sult2A1 induction
-
-
Manually annotated by BRENDA team
rabbit
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Wistar
-
-
Manually annotated by BRENDA team
pig
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenosine-5'-phosphosulfate + oestrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + glycolithocholate
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + isolithocholic acid
adenosine 3',5'-bisphosphate + isolithocholate sulfate
show the reaction diagram
-
5beta-cholanoic acid-3beta-ol
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + taurolithocholate
adenosine 3',5'-bisphosphate + taurolithocholate sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + 3beta-hydroxy 5-cholenoate
adenosine 3',5'-bisphosphate + 3beta-hydroxy 5-cholenoate monosulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + 5-cholenic acid-3beta-ol
adenosine 3',5'-bisphosphate + 5-cholenic acid-3-beta-ol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + 5alpha-cholanoic acid-3beta-ol
adenosine 3',5'-bisphosphate + 5alpha-cholanic acid-3beta-ol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + 5beta-scymnol
adenosine 3',5'-bisphosphate + 5beta-scymnol sulfate
show the reaction diagram
-
bile steroid
-
?
3'-phosphoadenylyl-sulfate + allocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + chenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + chenodeoxycholate
adenosine 3',5'-bisphosphate + chenodeoxycholate 7-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + cholic acid
adenosine 3',5'-bisphosphate + cholic acid sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + deoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + deoxycholate
adenosine 3',5'-bisphosphate + deoxycholate 12-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + glycine conjugated chenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated deoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated lithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + glycine conjugated ursodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + glycochenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + glycochenodeoxycholate
adenosine 3',5'-bisphosphate + glycochenodeoxycholate monosulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + glycocholate
adenosine 3',5'-bisphosphate + glycocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + glycolithocholate
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + hyodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + lithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl-sulfate + lithocholate
adenosine 3',5'-bisphosphate + lithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + petromyzonol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + phenol
adenosine 3',5'-bisphosphate + phenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated chenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated deoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated lithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurine conjugated ursodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurochenodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + taurochenodeoxycholate
adenosine 3',5'-bisphosphate + taurochenodeoxycholate monosulfate + taurochenodeoxycholate disulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + taurocholate
adenosine 3',5'-bisphosphate + taurocholate monosulfate + taurocholate disulfate + taurocholate trisulfate
show the reaction diagram
3'-phosphoadenylyl-sulfate + ursodeoxycholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl-sulfate + ursodeoxycholate
adenosine 3',5'-bisphosphate + ursodeoxycholate sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chenodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + cholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
lowest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + deoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + lithocholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
highest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + ursodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + glycolithocholate
adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
show the reaction diagram
Q06520
the enzyme mediates catalytic conversion of hydroxysteroids, bile acids, and certain clinical drugs to water-soluble sulfated metabolites
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + taurolithocholate
adenosine 3',5'-bisphosphate + taurolithocholate sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chenodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + cholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
lowest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
-
transcriptional regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) by estrogen-related receptor alpha
-
-
?
3'-phosphoadenylylsulfate + deoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + lithocholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
highest affinity to sulfation by SULT2A1
-
-
?
3'-phosphoadenylylsulfate + ursodeoxycholic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
activity 107%
CuCl2
-
activity 136%
CuSO4
-
activity 107%
FeSO4
-
activity 134%
KCl
-
activity 128%
LiCl
-
activity 108%
Zn2+
-
isoenzyme BSS I activity enhanced, BSS II activity inhibited
ZnSO4
-
activity 111%
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-Dichloro-4-nitrophenol
-
non-specific SULT inhibitor
3-keto-5-beta-cholanoate
-
competitive inhibitor of glycolithocholate
-
3-ketolithocholate
5,7-dichlorokynurenic acid
-
-
7,12-diketochenodeoxycholate
-
-
adenosine-3',5'-diphosphate
Co2+
-
isoenzyme BSS II
gavestinel
-
i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt
glycolithocholate
-
-
iodoacetate
isolithocholate
-
-
Kynurenic acid
-
-
L689,560
-
i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline
L701,324
-
i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone
Mefenamic acid
-
-
p-chloromercuribenzoate
Taurodehydrocholate
-
-
additional information
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
constitutive androstane receptor
-
3.5fold induction of SULT2A1 mRNAs in human cell lines of enterohepatic origin, such as Caco-2 intestinal cells and HepG2 hepatoma cells transfected with the CAR plasmid
-
estrogen
-
isoenzyme BSS I is stimulated by
pregnane X receptor
-
1.7fold induction of SULT2A1 mRNAs in human cell lines of enterohepatic origin, such as Caco-2 intestinal cells and HepG2 hepatoma cells transfected with the PXR plasmid
-
pregnenolone-16alpha-carbonitrile
-
aging does not change the induction of enzyme by synthetic steroid pregnenolone-16alpha-carbonitrile
vitamin D3
-
aging does not change the induction of enzyme by hormonally active vitamin D3
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007 - 0.0115
3'-phosphoadenylylsulfate
0.0024
3-beta-5-cholenic acid
-
pH 6.5, 37°C
-
0.071
3alpha,7alpha,12alpha-cholic acid
-
pH 6.5, 37°C
0.025
3alpha-7alpha-chenodeoxycholic acid
-
pH 6.5, 37°C
0.014
5beta-scymnol
-
-
0.01566 - 0.0423
chenodeoxycholate
0.01566
chenodeoxycholic acid
-
apparent value, at pH 5.5 and 7.4, at 37°C
0.0014 - 0.00383
dehydroepiandrosterone
0.00886
deoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00886
deoxycholic acid
-
apparent value, at pH 5.5 and 7.4, at 37°C
0.04899
glycine conjugated chenodeoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.05459
glycine conjugated deoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00192
glycine conjugated lithocholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.04957
glycine conjugated ursodeoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.03673
glycochenodeoxycholate
-
in 50 mM Mops buffer at pH 7.0, at 37°C
0.0018 - 0.02
glycolithocholate
0.002 - 0.00836
lithocholate
0.00202
lithocholic acid
-
apparent value, at pH 5.5 and 7.4, at 37°C
0.04938
Petromyzonol
-
in 50 mM Mops buffer at pH 7.0, at 37°C
0.04509
taurine conjugated chenodeoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.01836
taurine conjugated deoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00186
taurine conjugated lithocholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.00993
taurine conjugated ursodeoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.02 - 0.077
taurolithocholate
0.01286
ursodeoxycholate
-
apparent value, in 50 mM NaH2PO4 buffer (pH 5.5), 2 mM MgCl2, at 37°C
0.01286
Ursodeoxycholic acid
-
apparent value, at pH 5.5 and 7.4, at 37°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006 - 0.0033
3-ketolithocholate
0.0002
adenosine-3',5'-diphosphate
-
pH 6.5, 37°C
0.0022
glycolithocholate
-
pH 6.5, 37°C
0.026
isolithocholate
-
pH 6.5, 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00079
-
using hyodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00163
-
-
0.00248
-
using allochloate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C; using taurochenodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.0028
-
using glycochenodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00354
-
using chenodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00359
-
using petromyzonol as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.0049
-
kidney enzyme
0.00492
-
using ursodeoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.00819
-
using deoxycholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
0.018
-
-
0.0216
-
-
0.02361
-
using lithocholate as substrate, in 50 mM Mops buffer at pH 7.0, at 37°C
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9
-
-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
a more than 10fold increase in enzyme activity is observed with increasing the pH from 4.0 to 5.5, while further increase in pH in 10 mM Tris-HCl buffer is associated with a decline in the enzyme activity; more than a 10fold increase in SULT2A1 activity is observed with increasing the pH from 4.0 to 5.5, while a further increase in pH in 10 mM Tris-HCl buffer (up to pH 9-0) is associated with a 50% decline in the optimum enzyme activity
5 - 8
-
little activity recorded at pH 5.0 and pH 8.0
5.5 - 10.5
6.5 - 9
-
-
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectric focusing, 2 major bands with different pI values
5.2
-
isoelectric focusing electrophoresis
5.4
-
isoelectric focusing
5.6 - 5.9
-
-
5.8
-
kidney enzyme, isoelectric focusing
5.85 - 6.69
-
isoelectric focusing, 2 major bands with different pI values
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
no activity in microsomes
-
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
SDS-PAGE
32000
-
3 different peaks of bile salt sulfotransferase activity, glycochenodeoxycholate as substrate, gel filtration
33780
-
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence
34000
-
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence
40000
-
gel filtration
45000
-
3 different peaks of bile salt sulfotransferase activity, glycochenodeoxycholate as substrate, gel filtration
60000
-
3 different peaks of bile salt sulfotransferase activity, glycolithocholate as substrate, gel filtration
64000
-
3 different peaks of bile salt sulfotransferase activity, glycolithocholate as substrate, gel filtration
65000
-
gel filtration
67000
-
gel filtration
68000
-
gel filtration
76000
-
gel filtration
80000
-
kidney enzyme, gel filtration
130000
200000
-
3 different peaks of bile salt sulfotransferase activity, active with glycochenodeoxycholate and glycolithocholate, gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 34000, SDS-PAGE, x * 33780, calculated from amino acid sequence
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing results in significant loss in activity, substantial stabilization with addition of sucrose
-
repeated freezing and thawing reduces activity considerably, addition of ethylene glycol preserves activity
-
SULT2A1 activity is consistently higher using NaH2PO4 buffer in a pH range from 5.5 to 6.5
-
SULT2A1 activity is consistently highest using NaH2PO4 buffer in a pH range from 5.5 to 6.5
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, (NH4)2SO4 precipitate, stable for several months
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-20°C, crude enzyme may be stored for several months without loss of enzyme activity
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-20°C, when stored frozen, activity in enzyme homogenate is preserved for at least 9 months
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-30°C, ethylene glycol enzyme solution can be stored for at least 3 months without serious decrease in activity
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4°C, labile
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4°C, loses about 50% activity in 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
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partially, isoenzyme BSS II
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in HEK-293 cells; expressed in HEK293 cells
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expression in HepG2 cells or CaCo-2 cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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LINKS TO OTHER DATABASES (specific for EC-Number 2.8.2.14)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)