Information on EC 2.8.2.13 - psychosine sulfotransferase

for references in articles please use BRENDA:EC2.8.2.13
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The expected taxonomic range for this enzyme is: Mus musculus

EC NUMBER
COMMENTARY hide
2.8.2.13
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RECOMMENDED NAME
GeneOntology No.
psychosine sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + galactosylsphingosine = adenosine 3',5'-bisphosphate + psychosine sulfate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:galactosylsphingosine sulfotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37259-76-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mouse, normal and Jimpy mutant, maximum enzyme activity at 17-19 days after birth
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + galactosylsphingosine
adenosine 3',5'-bisphosphate + psychosine sulfate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + galactosylsphingosine
adenosine 3',5'-bisphosphate + psychosine sulfate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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divalent ions other than magnesium causes a reduction of enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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decreases the incorporation of sulfate ions into psychosine sulfate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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cysteine
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deoxycholate
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EDTA
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stimulatory effect
glutathione
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stimulates SO42- incorporation by 23%
Tween 20
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detergent is necessary for enzyme action in vitro, removing Tween 20 from the incubation medium inhibits enzyme activity 92.5%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
galactosylsphingosine
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pH 7.4, 37°C, psychosine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8
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about 50% of activity maximum at pH 6.4, about 60% of activity maximum at pH 7.4
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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hardly any effect in liver
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 55
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at 45°C enzyme activity is reduced by 85%, at 55°C activity is lost
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, microsomes or whole homogenate retains enzyme activity for at least 1 month
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