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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
Lst, luciferin sulfokinase, luciferin sulfokinase(3'-phosphoadenylyl sulfate:luciferin sulfotransferase), luciferin sulfotransferase,
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luciferin sulfokinase
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luciferin sulfokinase(3'-phosphoadenylyl sulfate:luciferin sulfotransferase)
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luciferin sulfotransferase
Lst
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luciferin sulfotransferase
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luciferin sulfotransferase
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luciferin sulfotransferase
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luciferin sulfotransferase
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luciferin sulfotransferase
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luciferin sulfotransferase
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3'-phosphoadenylyl sulfate + Renilla luciferin = adenosine 3',5'-bisphosphate + luciferyl sulfate
the product may be identical with Watasenia luciferin
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sulfate group transfer
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3'-phosphoadenylyl-sulfate:Renilla luciferin sulfotransferase
The product may be identical with Watasenia luciferin.
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3'-AMP + luciferyl sulfate
3'-AMP sulfate + luciferin
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3'-phosphoadenylyl sulfate + Cypridina luciferin
adenosine 3',5'-bisphosphate + Cypridina luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
5'-AMP + luciferyl sulfate
5'-AMP sulfate + luciferin
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adenosine 3',5'-bisphosphate + Cypridina luciferyl sulfate
3'-phosphoadenylyl sulfate + Cypridina luciferin
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benzyl luciferin + 3'-phosphoadenylylsulfate
benzyl luciferyl sulfate + adenosine 3',5'-bisphosphate
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fully active analog of coelenterate-type luciferin, equally effective as substrate
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benzyl luciferyl sulfate + adenosine 3',5'-bisphosphate
benzyl luciferin + 3'-phosphoadenylylsulfate
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CoA + luciferyl sulfate
CoA sulfate + luciferin
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Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
Renilla luciferin + 3'-phosphoadenylylsulfate
additional information
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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r
3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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r
3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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r
3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
Renilla luciferin + 3'-phosphoadenylylsulfate
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Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
Renilla luciferin + 3'-phosphoadenylylsulfate
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r
additional information
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enzyme is appparently specific for luciferin, does not exhibit any phenol sulfokinase activity
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additional information
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CoA can act as a sulfate acceptor in the sulfotransferase reaction, and can also competitively inhibit the sulfotransferase. Sulfotransferase(s) in Cypridina specimens can efficiently use CoA, 5'-AMP and 3'-AMP as a sulfate acceptor from Cypridina luciferyl sulfate and produce Cypridina luciferin, similar to the case with PAP. Mass spectral analyses of the sulfated products of 5'-AMP, 3'-AMP, and CoA
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3'-phosphoadenylyl sulfate + Cypridina luciferin
adenosine 3',5'-bisphosphate + Cypridina luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
adenosine 3',5'-bisphosphate + Cypridina luciferyl sulfate
3'-phosphoadenylyl sulfate + Cypridina luciferin
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Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
Renilla luciferin + 3'-phosphoadenylylsulfate
3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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3'-phosphoadenylyl sulfate + Renilla luciferin
adenosine 3',5'-bisphosphate + luciferyl sulfate
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Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
Renilla luciferin + 3'-phosphoadenylylsulfate
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Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
Renilla luciferin + 3'-phosphoadenylylsulfate
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r
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CoA
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CoA may be the competitive inhibitor against PAP in Cypridina sulfotransferase(s) and can also act as a sulfate acceptor
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brenda
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brenda
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UniProt
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brenda
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brenda
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brenda
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brenda
i.e. Vargula hilgendorfii
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i.e. Vargula hilgendorfii, collected at Chita (Aichi, Japan) on August 4, 2011, and Naruto (Tokushima, Japan) on August 24, 2017
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physiological function
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in Cypridina (Vargula) hilgendorfii, Cypridina luciferin is converted from Cypridina luciferyl sulfate by a sulfotransferase with adenosine 3',5'-diphosphate (PAP), and is used for the luminescence reaction of Cypridina luciferase. The luminescence activity of crude extracts of Cypridina hilgendorfii is significantly stimulated by the addition of acetic acid. This stimulation may be explained by an efficient supply of PAP from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) catalyzed by the sulfotransferase. Acetic acid acts as a sulfate acceptor from PAPS, followed by forming acetyl sulfate and PAP. The structure of acetyl sulfate is identified using mass spectrometry and it spontaneously decomposed to acetic acid and free sulfate ion in aqueous solutions. This enzymatic conversion from Cypridina luciferyl sulfate to Cypridina luciferin coupled with acetic acid and PAPS by the luciferin sulfotransferase, enzymatic cycling of Cypridina luciferyl sulfate to Cypridina luciferin coupled with acetic acid and PAPS
physiological function
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in the luminous ostracod Cypridina (presently Vargula) hilgendorfii, Cypridina luciferyl sulfate (3-enol sulfate of Cypridina luciferin) is converted to Cypridina luciferin by a sulfotransferase with 3'-phosphoadenosine-5'-phosphate (PAP) as a sulfate acceptor. The resultant Cypridina luciferin is used for the luciferase-luciferin reaction of Cypridina to emit blue light. the addition of CoA to the crude extracts significantly stimulates luminescence intensity. CoA can act as a sulfate acceptor from Cypridina luciferyl sulfate. In addition, the sulfate group of Cypridina luciferyl sulfate is also transferred to adenosine 5'-monophosphate (5'-AMP) and adenosine 3'-monophosphate (3'-AMP) by the sulfotransferase. Other adenosine derivatives of 5'-AMP and 3'-AMP can also act as efficient sulfate acceptors on the millimolar order from Cypridina luciferyl sulfate by the sulfotransferase and they stimulate light emission in crude extracts of Cypridina specimens. ATP, NADH, and NAD+ weakly stimulate the luminescence activity
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LST_PHOPY
370
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43274
Swiss-Prot
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activity is labile, can be stabilized by continous presence of 0.05 ml/l 2-mercaptoethanol
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-80°C, requirement of 2-mercaptoethanol in storage buffer, stable for at least several months
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partially by gel filtration and ultrafiltration
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Cormier, M.J.; Hori, K.; Karkhanis, Y.D.
Studies on the bioluminescence of Renilla reniformis. VII. Conversion of luciferin into luciferyl sulfate by luciferin sulfokinase
Biochemistry
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1184-1189
1970
Renilla reniformis
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Anderson, J.M.; Hori, K.; Cormier, M.J.
A bioluminescence assay for PAP(3',5'-diphosphoadenosine) and PAPS (3'-phosphoadenylyl sulfate)
Methods Enzymol.
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244-257
1978
Renilla reniformis
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Fallon, T.R.; Li, F.S.; Vicent, M.A.; Weng, J.K.
Sulfoluciferin is biosynthesized by a specialized luciferin sulfotransferase in fireflies
Biochemistry
55
3341-3344
2016
Ellychnia corrusca, Ignelater luminosus, Photinus pyralis (A0A173GP47), Photinus pyralis, Photuris, Pyractomena
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Nakamura, M.; Suzuki, T.; Ishizaka, N.; Sato, J.; Inouye, S.
Identification of 3-enol sulfate of Cypridina luciferin, Cypridina luciferyl sulfate, in the sea-firefly Cypridina (Vargula) hilgendorfii
Tetrahedron
70
2161-2168
2014
Vargula hilgendorfii
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Nakamura, M.; Matsuda, K.; Morikawa, T.; Ishizuka, K.; Inouye, S.
Efficient conversion to Cypridina luciferin from Cypridina luciferyl sulfate, coupled with enzymatic sulfation of acetic acid
Biochem. Biophys. Res. Commun.
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678-684
2020
Vargula hilgendorfii
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Nakamura, M.; Matsuda, K.; Nakamura, M.; Yamashita, K.; Suzuki, T.; Inouye, S.
Enzymatic conversion of Cypridina luciferyl sulfate to Cypridina luciferin with coenzyme A as a sulfate acceptor in Cypridina (Vargula) hilgendorfii
Photochem. Photobiol.
95
1376-1386
2019
Vargula hilgendorfii
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