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Information on EC 2.8.2.1 - aryl sulfotransferase and Organism(s) Canis lupus familiaris and UniProt Accession Q29476
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EC Tree
2.8.2.1 aryl sulfotransferaseIUBMB Comments
A number of aromatic compounds can act as acceptors. Organic hydroxylamines are not substrates (cf. EC 2.8.2.9 tyrosine-ester sulfotransferase).
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Word Map
- 2.8.2.1
-
sulfotransferases
-
sults
- xenobiotics
- estrogen
- sulphate
- steroid
- dehydroepiandrosterone
- hepatocytes
-
glucuronidation
- 3'-phosphoadenosine
- hydroxysteroids
- dheas
-
udp-glucuronosyltransferase
- 5'-phosphosulfate
- ugt1a1
-
drug-metabolizing
- cyp1a2
- 3'-phosphoadenosine-5'-phosphosulfate
- 4-nitrophenol
- n-acetyltransferase
-
procarcinogens
-
sulfatase
-
allozymes
- pregnenolone
-
sulfurylation
-
androstane
-
sulfoconjugation
-
pregnane
- cyp2d6
- ugt2b7
-
arylamines
- 3'-phosphate
-
farnesoid
- pentachlorophenol
- synthesis
-
cdna-expressed
- analysis
-
promutagens
-
n-hydroxylated
-
2-amino-1-methyl-6-phenylimidazo4,5-bpyridine
- oestrone
-
cyp2b10
- n-hydroxy-2-acetylaminofluorene
-
glucuronyltransferase
-
n-hydroxy
-
xenobiotic-metabolizing
-
35ssulfate
-
monosulfate
-
iodothyronine
- hsd17b1
- medicine
- cyp3a11
The taxonomic range for the selected organisms is: Canis lupus familiaris
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
sult1a1, sult2a1, sult1e1, sulphotransferase, sult1a3, phenol sulfotransferase, cytosolic sulfotransferase, sult4a1, sult1b1, sult1a2, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1-naphthol phenol sulfotransferase
-
-
-
-
2-naphtholsulfotransferase
-
-
-
-
4-nitrocatechol sulfokinase
-
-
-
-
arylsulfotransferase
-
-
-
-
ASTIV
-
-
-
-
catecholamine-sulfating phenol sulfotransferase
-
-
-
-
DOPA/tyrosine sulfotransferase
-
-
-
-
dopamine sulfotransferase
-
-
-
-
HAST1/HAST2
-
-
-
-
HAST3
-
-
-
-
hippocampal phenol sulfotransferase
-
-
-
-
M-PST
-
-
-
-
minoxidil sulfotransferase
-
-
-
-
monoamine sulfotransferase
-
-
-
-
p-nitrophenol sulfotransferase
-
-
-
-
P-PST
-
-
-
-
phenol sulfokinase
-
-
-
-
phenol sulfotransferase
-
-
-
-
phenol/aryl sulfotransferase
-
-
-
-
Pst
-
-
-
-
ritodrine sulfotransferase
-
-
-
-
ST1A2
-
-
-
-
ST1A3
-
-
-
-
ST1A4
-
-
-
-
sulfokinase
-
-
-
-
sulfotransferase, aryl
-
-
-
-
sulfotransferase, monoamine-preferring
-
-
-
-
thermolabile phenol sulfotransferase
-
-
-
-
thermostable phenol sulfotransferase
-
-
-
-
TL-PST
-
-
-
-
TS-PST
-
-
-
-
tyrosine-ester sulfotransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
mechanism
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:phenol sulfotransferase
A number of aromatic compounds can act as acceptors. Organic hydroxylamines are not substrates (cf. EC 2.8.2.9 tyrosine-ester sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY
9026-09-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
SULT1A1
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + ?
SULT1A1
-
-
?
3'-phosphoadenylylsulfate + minoxidil
adenosine 3',5'-bisphosphate + minoxidil sulfate
SULT1A1
-
-
?
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
-
-
-
?
3'-phosphoadenylylsulfate + 2-hydroxyestradiol
adenosine 3',5'-bisphosphate + estradiol 2-sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-hydroxyestrone
adenosine 3',5'-bisphosphate + estrone 2-sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
3'-phosphoadenylylsulfate + adrenaline
adenosine 3',5'-bisphosphate + adrenaline sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + norepinephrine
adenosine 3',5'-bisphosphate + norepinephrine sulfate
-
i.e. noradrenalin
i.e. noradrenalin sulfate
?
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
-
-
?
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
-
-
r
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
SULT1A1
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
-
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
SULT1A1
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
enzyme form SULT1D1
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
-
enzyme form SULT1D1
-
-
?
additional information
?
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
-
no activity with dehydroepiandrosterone
-
-
?
additional information
?
-
different enzyme forms show overlapping substrate specificities, overview
-
-
?
additional information
?
-
-
isoenzyme B catalyzes the sulfurylation of a wider range of substrates than A which is preferentially active with dopamine
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KCl
-
50 mM, 60% inhibition of isoenzyme A, slight activation of isoenzyme B
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0017
3'-phosphoadenylylsulfate
-
with dopamine, isoenzyme A, pH 6.0, 37°C
0.026
3'-phosphoadenylylsulfate
-
with dopamine, isoenzyme B, pH 9.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.061
dopamine
-
substrate inhibition, wild-type enzyme, enzyme form SULT1D1
0.063
4-nitrophenol
-
substrate inhibition, mutant enzyme D247L, enzyme form SULT1D1
1
4-nitrophenol
-
substrate inhibition, wild-type enzyme, enzyme form SULT1D1
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
P-PST, 100% identical in amino acid sequence with GenBank accession number AY069922
SwissProt
SULT1A1, 100% identical in amino acid sequence with GenBank accession number D29807
SwissProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ST1A1_CANLF
295
0
34115
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A146D
-
sulfation activity of the mutant is strongly decreased for the substrates 4-nitrophenol and dopamine, enzyme form SULT1D1
A146Q
-
sulfation activity of the mutant is strongly decreased for the substrates 4-nitrophenol and dopamine, enzyme form SULT1D1
D247L
-
21fold better sulfation of 4-nitrophenol (120fold decrease in KM-value) and 54fold less efficient in sulfation of dopamine (8fold increase in KM-value) compared to wild-type enzyme. Preference is switched from dopamine to 4-nitrophenol, enzyme form SULT1D1
I86D
-
sulfation activity of the mutant is strongly decreased for the substrates 4-nitrophenol and dopamine, enzyme form SULT1D1
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli BL21(DE3) as His-tagged protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Romain, Y.; Demassieux, S.; Carriere, S.
Partial purification and characterization of two isoenzymes involved in the sulfurylation of catecholamines
Biochem. Biophys. Res. Commun.
106
999-1005
1982
Canis lupus familiaris
Tsoi, C.; Morgenstern, R.; Swedmark, S.
Canine sulfotransferase SULT1A1: molecular cloning, expression, and characterization
Arch. Biochem. Biophys.
401
125-133
2002
Canis lupus familiaris (Q29476), Canis lupus familiaris
Duffel, M.W.; Marshall, A.D.; McPhie, P.; Sharma, V.; Jakoby, W.B.
Enzymatic aspects of the phenol (aryl) sulfotransferases
Drug Metab. Rev.
33
369-395
2001
Bos taurus, Homo sapiens, Homo sapiens (O00338), Homo sapiens (O43704), Homo sapiens (O75897), Mus musculus, Mus musculus (Q3UZZ6), Mus musculus (Q80VR3), Mus musculus (Q9QWG7), Rattus norvegicus, Rattus norvegicus (P50237), Rattus norvegicus (P52847), Rattus norvegicus (Q9WUW8), Rattus norvegicus (Q9WUW9), Oryctolagus cuniculus (O46503), Oryctolagus cuniculus (Q9XT99), Macaca fascicularis (P52846), Canis lupus familiaris (Q29476), Oryctolagus cuniculus ST1A8 (Q9XT99), Oryctolagus cuniculus ST1C5 (O46503), Mus musculus ST1c4 (Q80VR3), Macaca fascicularis ST1A9 (P52846), Mus musculus ST1a4
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