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Information on EC 2.8.2.1 - aryl sulfotransferase and Organism(s) Bos taurus and UniProt Accession P50227

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.1 aryl sulfotransferase
IUBMB Comments
A number of aromatic compounds can act as acceptors. Organic hydroxylamines are not substrates (cf. EC 2.8.2.9 tyrosine-ester sulfotransferase).
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This record set is specific for:
Bos taurus
UNIPROT: P50227
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The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
sult1a1, sult2a1, sult1e1, sulphotransferase, sult1a3, phenol sulfotransferase, cytosolic sulfotransferase, sult4a1, sult1b1, sult1a2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phenol sulfotransferase
-
1-naphthol phenol sulfotransferase
-
-
-
-
2-naphtholsulfotransferase
-
-
-
-
4-nitrocatechol sulfokinase
-
-
-
-
arylsulfotransferase
-
-
-
-
ASTIV
-
-
-
-
catecholamine-sulfating phenol sulfotransferase
-
-
-
-
DOPA/tyrosine sulfotransferase
-
-
-
-
dopamine sulfotransferase
-
-
-
-
HAST1/HAST2
-
-
-
-
HAST3
-
-
-
-
hippocampal phenol sulfotransferase
-
-
-
-
M-PST
-
-
-
-
minoxidil sulfotransferase
-
-
-
-
monoamine sulfotransferase
-
-
-
-
p-nitrophenol sulfotransferase
-
-
-
-
P-PST
-
-
-
-
phenol sulfokinase
-
-
-
-
phenol sulfotransferase
-
-
-
-
phenol/aryl sulfotransferase
-
-
-
-
Pst
-
-
-
-
ritodrine sulfotransferase
-
-
-
-
ST1A2
-
-
-
-
ST1A3
-
-
-
-
ST1A4
-
-
-
-
sulfokinase
-
-
-
-
sulfotransferase, aryl
-
-
-
-
sulfotransferase, monoamine-preferring
-
-
-
-
thermolabile phenol sulfotransferase
-
-
-
-
thermostable phenol sulfotransferase
-
-
-
-
TL-PST
-
-
-
-
TS-PST
-
-
-
-
tyrosine-ester sulfotransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:phenol sulfotransferase
A number of aromatic compounds can act as acceptors. Organic hydroxylamines are not substrates (cf. EC 2.8.2.9 tyrosine-ester sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9026-09-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin
adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
-
ST1A6
-
-
?
3'-phosphoadenylylsulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acyl-CoA
weak inhibition
CoA
reversible covalent inhibition, the inactivation of the dimeric enzyme is stoichiometric, binding structure and mechanism, overview
coenzyme A
-
palmitoyl-CoA
-
2,6-Dichloro-4-nitrophenol
-
-
chlorpromazine
-
-
pyridoxal 5'-phosphate
-
50% inhibition at 0.001 mM, excess of 2-naphthol protects
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000815 - 0.00524
3'-phosphoadenylyl sulfate
0.000526 - 0.003
7-hydroxycoumarin
additional information
additional information
-
kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.069 - 0.18
3'-phosphoadenylyl sulfate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13 - 173
3'-phosphoadenylyl sulfate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021 - 0.00573
CoA
0.00577
coenzyme A
-
0.023
pyridoxal 5'-phosphate
-
-
additional information
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
5.7
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ST1A1_BOVIN
295
0
34184
Swiss-Prot
other Location (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modeling of the active site. Two phenylalanyl and one tyrosinyl group help create a hydrophobic pocket for phenolic acceptor substrates. Two arginyl and one lysyl residues, plus other groups, aid in the binding of 3'-phosphoadenosine-5'-phospho(sulfate) via electrostatic forces and H-bonding. The conserved histidine serves as the general base to initiate phenoxide anion stabilization and attack on the 3'-phosphoadenosine-5'-phosphosulfate sulfuryl group
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
W53A
mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol
W53L
mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol
W53Y
mutation decreases thermal stabilizations by 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42 - 45
30 min, 50% inactivation, wild-type and mutants
45
melting temperature, wild-type peaks at 45°C, with a domain-suggestive shoulder at 49°C
60
melting temperature, wild-type in presence of 3'-phosphoadenosine-5'-phosphate plus pentachlorophenol
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-cellulose column chromatography
recombinant SULT1A1 from Escherichia coli strain DH5alpha by anion exchange chromatography and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of pTrc99A-bSULT1A1 in Escherichia coli DH5alpha
SULT1A1, expression in Escherichia coli strain DH5alpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bartzatt, R.; Beckmann, J.D.
Inhibition of phenol sulfotransferase by pyridoxal phosphate
Biochem. Pharmacol.
47
2087-2095
1994
Bos taurus
Manually annotated by BRENDA team
Fernando, P.H.P.; Sakakibara, Y.; Nakatsu, S.; Suiko, M.; Han, J.R.; Liu, M.C.
Isolation and characterization of a novel microsomal membrane-bound phenol sulfotransferase from bovine liver
Biochem. Mol. Biol. Int.
30
433-441
1993
Bos taurus
Manually annotated by BRENDA team
Duffel, M.W.; Marshall, A.D.; McPhie, P.; Sharma, V.; Jakoby, W.B.
Enzymatic aspects of the phenol (aryl) sulfotransferases
Drug Metab. Rev.
33
369-395
2001
Bos taurus, Homo sapiens, Homo sapiens (O00338), Homo sapiens (O43704), Homo sapiens (O75897), Mus musculus, Mus musculus (Q3UZZ6), Mus musculus (Q80VR3), Mus musculus (Q9QWG7), Rattus norvegicus, Rattus norvegicus (P50237), Rattus norvegicus (P52847), Rattus norvegicus (Q9WUW8), Rattus norvegicus (Q9WUW9), Oryctolagus cuniculus (O46503), Oryctolagus cuniculus (Q9XT99), Macaca fascicularis (P52846), Canis lupus familiaris (Q29476), Oryctolagus cuniculus ST1A8 (Q9XT99), Oryctolagus cuniculus ST1C5 (O46503), Mus musculus ST1c4 (Q80VR3), Macaca fascicularis ST1A9 (P52846), Mus musculus ST1a4
Manually annotated by BRENDA team
Chodavarapu, S.; Hertema, H.; Huynh, T.; Odette, J.; Miller, R.; Fullerton, A.; Alkirwi, J.; Hartsfield, D.; Padmanabhan, K.; Woods, C.; Beckmann, J.D.
Reversible covalent inhibition of a phenol sulfotransferase by coenzyme A
Arch. Biochem. Biophys.
457
197-204
2007
Bos taurus (P50227), Bos taurus
Manually annotated by BRENDA team
Beckmann, J.; Chodavarapu, S.; Doyle, B.
Evaluation of a conserved tryptophanyl residue in donor substrate binding and catalysis by a phenol sulfotransferase (SULT1A1)
Arch. Biochem. Biophys.
695
108621
2020
Bos taurus (P50227), Bos taurus
Manually annotated by BRENDA team