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Information on EC 2.8.2.1 - aryl sulfotransferase and Organism(s) Rattus norvegicus and UniProt Accession P17988

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.1 aryl sulfotransferase
IUBMB Comments
A number of aromatic compounds can act as acceptors. Organic hydroxylamines are not substrates (cf. EC 2.8.2.9 tyrosine-ester sulfotransferase).
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Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: P17988
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
sult1a1, sult2a1, sult1e1, sulphotransferase, sult1a3, phenol sulfotransferase, cytosolic sulfotransferase, sult4a1, sult1b1, sult1a2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aryl sulfotransferase IV
-
phenol sulfotransferase 1A1
-
1-naphthol phenol sulfotransferase
-
-
-
-
2-naphtholsulfotransferase
-
-
-
-
4-nitrocatechol sulfokinase
-
-
-
-
aryl SULT
-
-
arylsulfotransferase
-
-
-
-
ASTIV
-
-
-
-
catecholamine-sulfating phenol sulfotransferase
-
-
-
-
DOPA/tyrosine sulfotransferase
-
-
-
-
dopamine sulfotransferase
-
-
-
-
HAST1/HAST2
-
-
-
-
HAST3
-
-
-
-
hippocampal phenol sulfotransferase
-
-
-
-
M-PST
-
-
-
-
minoxidil sulfotransferase
-
-
-
-
monoamine sulfotransferase
-
-
-
-
p-nitrophenol sulfotransferase
-
-
-
-
P-PST
-
-
-
-
phenol sulfokinase
-
-
-
-
phenol sulfotransferase
-
-
-
-
phenol/aryl sulfotransferase
-
-
-
-
Pst
-
-
-
-
ritodrine sulfotransferase
-
-
-
-
ST1A2
-
-
-
-
ST1A3
-
-
-
-
ST1A4
-
-
-
-
sulfokinase
-
-
-
-
sulfotransferase 1A1
-
-
sulfotransferase, aryl
-
-
-
-
sulfotransferase, monoamine-preferring
-
-
-
-
thermolabile phenol sulfotransferase
-
-
-
-
thermostable phenol sulfotransferase
-
-
-
-
TL-PST
-
-
-
-
TS-PST
-
-
-
-
tyrosine-ester sulfotransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:phenol sulfotransferase
A number of aromatic compounds can act as acceptors. Organic hydroxylamines are not substrates (cf. EC 2.8.2.9 tyrosine-ester sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9026-09-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylyl sulfate + phenol
adenosine 3',5'-bisphosphate + phenyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylyl sulfate + silybin B
adenosine 3',5'-bisphosphate + silybin B 20-O-sulfate
show the reaction diagram
-
-
-
?
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
3'-phosphoadenylyl sulfate + 4-nitrophenol
show the reaction diagram
-
-
-
r
3'-phosphoadenylyl sulfate + 2,6-dichloro-4-nitrophenol
adenosine 3',5'-bisphosphate + 2,6-dichloro-4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 4-methylumbelliferone
adenosine 3',5'-bisphosphate + 4-methylumbelliferyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7-hydroxycoumarin
adenosine 3',5'-bisphosphate + 7-hydroxycoumarin sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + pentachlorophenol
adenosine 3',5'-bisphosphate + pentachlorophenyl sulfate
show the reaction diagram
low activity, inhibitory
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + quercetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + taxifolin
adenosine 3',5'-bisphosphate + taxifolin 3'-O-sulfate + quercetin 3'-O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + (-)-(1R,2R)-pseudoephedrine
adenosine 3',5'-bisphosphate + 2-(methylamino)-1-phenylpropyl sulfate
show the reaction diagram
-
absolute stereospecificity
-
-
?
3'-phosphoadenylylsulfate + (-)-(1R,2S)-ephedrine
adenosine 3',5'-bisphosphate + 2-(methylamino)-1-phenylpropyl sulfate
show the reaction diagram
-
absolute stereospecificity
-
-
?
3'-phosphoadenylylsulfate + (-)-(S)-2-methyl-1-phenyl-1-propanol
adenosine 3',5'-bisphosphate + 2-methyl-1-phenylpropyl 1-sulfate
show the reaction diagram
-
absolute stereospecificity
-
-
?
3'-phosphoadenylylsulfate + 1,2,3,4-tetrahydro-1-naphthol
adenosine 3',5'-bisphosphate + 1,2,3,4-tetrahydronaphthyl 1-sulfate
show the reaction diagram
-
only the (-)-(R)-enantiomer is active as substrate
-
-
?
3'-phosphoadenylylsulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
SULT1C2 and SULT1C2A: no activity
-
-
?
3'-phosphoadenylylsulfate + 1-phenylethanol
adenosine 3',5'-bisphosphate + 1-phenylethyl sulfate
show the reaction diagram
-
(+)-(R)- and (-)-(S)-
-
-
?
3'-phosphoadenylylsulfate + 2-amino-4'-hydroxy-1-methyl-6-phenylimidazo[4,5-b]pyridine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 2-chlorophenol
adenosine 3',5'-bisphosphate + 2-chlorophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 3,3',5'-triiodo-L-thyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 3,3',5-triiodo-D-thyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 3,3',5-triiodo-L-thyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylacetate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylglycol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3,5-diiodo-L-thyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
low activity
-
-
?
3'-phosphoadenylylsulfate + 3-chlorophenol
adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-hydroxybenzo[a]pyrene
adenosine 3',5'-bisphosphate + benzo[a]pyrene 3-sulfate
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 3-hydroxyindole
adenosine 3',5'-bisphosphate + 3-indoxyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylethyleneglycol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-methylphenol
adenosine 3',5'-bisphosphate + 3-methylphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-nitrophenol
adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-acetamidophenol
adenosine 3',5'-bisphosphate + 4-acetamidophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-chlorophenol
adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3-methoxyphenylglycol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 4-methoxyphenol
adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-methylphenol
adenosine 3',5'-bisphosphate + 4-methylphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-methylumbelliferone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 5,7-dihydroxyflavanone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 5-(hydroxyphenyl)-5-(p-tolyl)-hydantoin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 5-hydroxyindole
adenosine 3',5'-bisphosphate + 5-indoxyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 5-hydroxytryptamine
adenosine 3',5'-bisphosphate + 3-(2-aminoethyl)-5-indoxyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 5-hydroxytryptophol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + 6-hydroxymelatonin
adenosine 3',5'-bisphosphate + melatonin 6-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + acetaminophen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
SULT1C2 and SULT1C2A: no activity
-
-
?
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + catecholamine metabolites
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
deaminated and (or) O-methylated
-
-
?
3'-phosphoadenylylsulfate + D-3,4-dihydroxyphenylalanine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + DL-tyrosine
adenosine 3',5'-bisphosphate + DL-tyrosyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + epinephrine
adenosine 3',5'-bisphosphate + epinephrine sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + harmol
adenosine 3',5'-bisphosphate + harmol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + homovanillic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + hydroquinone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + hydroxybiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + L-3,4-dihydroxyphenylalanine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + L-tyrosine
adenosine 3',5'-bisphosphate + L-tyrosyl O4-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + minoxidil
adenosine 3',5'-bisphosphate + minoxidil sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + naringenin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + norepinephrine
adenosine 3',5'-bisphosphate + norepinephrine sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + normetanephrine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + phenol
adenosine 3',5'-bisphosphate + phenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + phentolamine
adenosine 3',5'-bisphosphate + phentolamine sulfate
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + sakuranetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + salicylamide
adenosine 3',5'-bisphosphate + 2-sulfooxybenzamide
show the reaction diagram
3'-phosphoadenylylsulfate + serotonin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + troglitazone
adenosine 3',5'-bisphosphate + troglitazone sulfate
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + tyramine
adenosine 3',5'-bisphosphate + tyramine O-sulfate
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + umbelliferone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
ST1A1
-
-
?
3'-phosphoadenylylsulfate + vanillin
adenosine 3',5'-bisphosphate + 3-methoxybenzaldehyde 4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + vanillylmandelic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
adenosine 2',3'-cyclic phosphate 5'-phosphate + 4-nitrophenyl sulfate
adenosine 2',3'-cyclic phosphate 5'-phosphate 5'-sulfate + 4-nitrophenol
show the reaction diagram
-
sulfation at 5'-position
-
?
adenosine 2',5'-bisphosphate + 4-nitrophenyl sulfate
adenosine 2'-phosphate 5'-phosphate 5'-sulfate + 4-nitrophenol
show the reaction diagram
-
sulfation at 5'-position
-
?
AMP + 4-nitrophenyl sulfate
adenosine 5'-phosphate 5'-sulfate + 4-nitrophenol
show the reaction diagram
-
sulfation at 5'-position
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + phenol
adenosine 3',5'-bisphosphate + phenyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylyl sulfate + silybin B
adenosine 3',5'-bisphosphate + silybin B 20-O-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-hydroxyindole
adenosine 3',5'-bisphosphate + 3-indoxyl sulfate
show the reaction diagram
-
in vivo function may include the production of the normal tryptophan metabolite indican
-
-
?
3'-phosphoadenylylsulfate + a phenol
adenosine 3',5'-bisphosphate + an aryl sulfate
show the reaction diagram
additional information
?
-
estradiol exhibits neuroprotective action in the brain e.g. by activity of SULT1A1 and other phase II antioxidant enzymes, regulation mechanisms, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3'-phosphoadenylyl sulfate
adenosine 3',5'-bisphosphate
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
-
50 mM, 60% inhibition of isoenzyme A, slight activation of isoenzyme B, aryl sulfotransferase I and II are activated appreciably in the presence of 0.5 M KCl or NaCl
NaCl
-
aryl sulfotransferase I and II are activated appreciably in the presence of 0.5 M NaCl or KCl
additional information
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-(R)-2-methyl-1-phenyl-1-propanol
-
competitive to 1-naphthalene methanol
(+)-(S)-1,2,3,4-Tetrahydro-1-naphthol
-
competitive to 1-naphthalene methanol
2,6-Dichloro-4-nitrophenol
3,3',5-triiodo-L-thyronine
substrate inhibition at 0.25 mM
3,4-dihydroxymandelic acid
-
-
3,4-dihydroxyphenylacetic acid
-
-
3,4-dihydroxyphenylethyleneglycol
-
-
3-Methoxy-4-hydroxyphenethanol
-
-
3-methoxy-4-hydroxyphenylacetic acid
-
-
3-methoxy-4-hydroxyphenylethyleneglycol
-
-
4-nitrophenol
adenosine 3',5'-bisphosphate
-
-
ADP
-
isoenzyme I
ATP
-
isoenzyme I
Cibacron blue
-
isozyme I
Cremophor EL
-
Ki of 3.24 mg/ml at pH 6.2 and 38°C
-
Cremophor ELP
-
-
-
Cremophor RH40
-
-
-
Cremophor RH60
-
-
-
Pentachlorophenol
sodium lauryl sulfate
-
Ki of 0.22 mg/ml at pH 6.2 and 38°C
Tween 20
-
-
Tween 80
-
Ki of 6.43 mg/ml at pH 6.2 and 38°C
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 3',5'-bisphosphate
-
adenosine 3',5'-bisphosphate
-
the beta-enzyme form shows a hyperbolic dependence on adenosine 3',5'-bisphosphate concentration, whereas the alpha-enzyme form is not stimulated
Diamide
-
i.e. 1,1'-azobis(N,N-dimethylformamide), diamide significantly increases the sulfation of 7-hydroxycoumarin at an optimal concentration of 0.005 mM
GSH
-
increases activity
tert-butyl hydroperoxide
-
tert-butyl hydroperoxide significantly increases the sulfation of 7-hydroxycoumarin at an optimal concentration of 0.01 mM
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
2-chlorophenol
-
isoenzyme I, pH 6.5, 37°C
0.06 - 0.09
2-naphthol
0.0065 - 0.012
3'-phosphoadenylylsulfate
0.017
3,4-dihydroxyphenylglycol
-
pH 6.0, 37°C
0.12 - 0.16
3-chlorophenol
0.07 - 0.08
3-hydroxyindole
0.018
3-methoxy-4-hydroxyphenylglycol
-
pH 6.4, 37°C
1.4
3-methylphenol
-
isoenzyme II, pH 6.5, 37°C
0.44 - 1
3-Nitrophenol
1.6 - 2.1
4-acetamidophenol
1.2 - 1.5
4-Chlorophenol
0.18 - 0.25
4-Hydroxybiphenyl
4.2 - 6.5
4-methoxyphenol
2.2 - 2.9
4-Methylphenol
0.0038 - 30.9
4-nitrophenol
0.29 - 0.3
5-hydroxyindole
0.53 - 1.6
5-hydroxytryptamine
0.03
6-hydroxymelatonin
0.0023
adenosine 2',3'-cyclic phosphate 5'-phosphate
-
pH 7.0, 25°C
0.067
adenosine 2',5'-bisphosphate
-
pH 7.0, 25°C
0.0004
adenosine 3',5'-bisphosphate
-
pH 7.0, 25°C
4.3
adenosine 5'-monophosphate
-
pH 7.0, 25°C
3.44
D-3,4-dihydroxyphenylalanine
pH 9.3, 37°C
0.01
dihydroxyphenylacetic acid
-
pH 5.8, 37°C
0.02 - 0.24
dopamine
0.014
homovanillic acid
-
pH 5.8, 37°C
0.6 - 1.8
hydroquinone
0.76
L-3,4-dihydroxyphenylalanine
pH 9.3, 37°C
0.1
norepinephrine
-
pH 9.0, 37°C
0.04 - 2.6
phenol
0.1
vanillylmandelic acid
-
pH 6.2, 37°C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.733
2-chlorophenol
-
isoenzyme I
0.783 - 0.9
2-naphthol
0.65 - 0.733
3-hydroxyindole
0.667 - 0.8
4-Chlorophenol
1.55 - 2
4-methoxyphenol
0.6 - 0.65
5-hydroxyindole
0.01 - 0.025
5-hydroxytryptamine
0.113 - 0.122
phenol
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0117 - 0.0125
SULT1A1, untreated male and female rat striata
0.0199 - 0.0239
SULT1A1, estradiol-treated male and female rat striata
0.0022
purified enzyme
0.25
-
arylsulfotransferase I
0.282
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
with substrate 3,4-dihydroxyphenylglycol
6.2
-
with substrate vanillylmandelic acid
6.4
-
with substrate 3-methoxy-4-hydroxyphenylglycol
7.4
-
assay at
9
-
with substrate dopamine or norepinephrine
9.5
-
substrate 2-naphthol, second optimum at pH 6.5
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SULT1A1, mainly striatum, lower contents in cortex, hippocampus, and cerebellum
Manually annotated by BRENDA team
-
activity is very low in comparison to activity in rat liver. In most cases the activities are present throughout the entire intestine and not restricted to a particular portion of this organ
Manually annotated by BRENDA team
-
all SULT1 isoforms are detected in the medulla oblongata and thalamus. Constitutive mRNA expression of SULT1A1 is detected in all eight brain regions. Isoform SULT2B1b mRNA expression level in the medulla oblongata is 1.7fold higher than that in the liver
Manually annotated by BRENDA team
-
all SULT1 isoforms are detected in the medulla oblongata and thalamus
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ST1A1_RAT
291
0
33906
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
x * 33000, SDS-PAGE
33500
-
2 * 33500, arylsulfotransferase III, SDS-PAGE
34000
gel filtration
35000
-
2 * 35000, SDS-PAGE
61000
-
arylsulfotransferase III, gel filtration
64000
-
arylsulfotransferase I or II, gel filtration
64000 - 65000
-
gel filtration
68000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 33000, SDS-PAGE
dimer
additional information
-
alpha- and beta-enzyme forms assemble through different folding processes
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
reconstitution of the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source, method optimization, overview. Specific sulfation transformation systems are successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4-sulfotransferase (C4ST, EC 2.8.2.5) and chondroitin 6-sulfotransferase (C6ST, EC 2.8.2.17). Chondroitin sulfate A and C are enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The ASST IV enzyme is used for PAPS regeneration. Purification of CS disaccharides by anion exchange chrmatography and NMR analysis of the composition of CS disaccharides
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
4°C, 82 h, 60% loss of activity
645654
8
-
4°C, 82 h, 80% loss of activity
645654
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol reduces activity
-
sucrose and 2-mercaptoethanol stabilize during purification
-
transferase II is unstable to isoelectric focusing
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Glycerol
the addition of 10% glycerol (v/v) can double the storage stability of the enzyme
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
aryl sulfotransferase IV is sensitive to oxidation, which causes an increase in sulfation of 4-nitrophenol, but also a shift of pH-optimum at physiological pH
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 4 days, 50% loss of activity
37°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 6 h, complete loss of activity
4°C, 50 mM potassium phosphate buffer, 150 mM KCl, 300 mM imidazole, pH7.5, 1 day, 50% loss of activity
-20°C, 7 days, 20% loss of activity of partially purified enzyme
-
-80°C, storage over night, 10%, transferase I, and 35%, transferase II, loss of activity
-
-80°C, transferase III may deteriorate at any stage of purification
-
4°C, sodium phosphate, pH 7.0, 0.25 M sucrose, 5 mM 2-mercaptoethanol, 3 mM NaN3, protein concentration 0.5-1 mg/ml, transferase I, II lose about 5% of their activity per week
-
4°C, stable for at least 2 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+NTA agarose column chromatography and Superdex 200 gel filtration
recombinant SULT1A1 from Escherichia coli strain BL21(DE3) cytosol by ammonium sulfate fractionation, anion exchange and adenosine 3',5'-bisphosphate affinity chromatography
30fold from brain
-
764fold, to homogeneity
arylsulfotransferase I, II, III
-
isoenzyme I and II
-
PAP-agarose affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
gene Sult1a1, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with C4ST and C6ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification
SULT1A1 from brain, expression in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, transient expression of Dopa/tyrosine sulfotransferase in COS-7 cells and Escherichia coli XL1-Blue
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)Gold cells
-
expression in Escherichia coli M15 as His-tagged protein
-
overexpression of alpha- and beta-enzyme form in Escherichia coli, at high expression levels, the beta-enzyme form becomes the majority, while the alpha-enzyme form increases only slightly compared to low level expression
-
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
the alpha-enzyme form renatures in presence of adenosine 3',5'-bisphosphate, while the beta-enzyme form renature only in absence of adenosine 3',5'-bisphosphate
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with chondroitin 4-sulfotransferase and chondroitin 6-sulfotransferase, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sekura, R.D.; Duffel, M.W.; Jakoby, W.B.
Aryl sulfotransferases
Methods Enzymol.
77
197-206
1981
Rattus norvegicus
Manually annotated by BRENDA team
Sekura, R.D.; Jakoby, W.B.
Phenol sulfotransferases
J. Biol. Chem.
254
5658-5663
1979
Rattus norvegicus
Manually annotated by BRENDA team
Seah, V.M.Y.; Wong, K.P.
2,6-Dichloro-4-nitrophenol (DCNP), an alternate-substrate inhibitor of phenolsulfotransferase
Biochem. Pharmacol.
47
1743-1749
1994
Rattus norvegicus
Manually annotated by BRENDA team
Rao, S.I.; Duffel, M.W.
Benzylic alcohols as stereospecific substrates and inhibitors for aryl sulfotransferase
Chirality
3
104-111
1991
Rattus norvegicus
Manually annotated by BRENDA team
Baranczyk-Kuzma, A.; Borchardt, R.T.; Schasteen, C.S.; Pinnick, C.L.
Phenol sulfotransferases: purification and characterization of the rat brain enzyme
Phenolsulfotransferase, Ment. Health Res. (Sandler, M. , Usdin, E. Eds. ) Macmillan
London
55-73
1981
Rattus norvegicus
-
Manually annotated by BRENDA team
Pennings, E.J.M.; Vrielink, R.; Wolters, W.L.; van Kempen, G.M.J.
Inhibition of rat brain phenol sulphotransferase in vitro by noradrenaline and dopamine metabolites
J. Neurochem.
27
915-920
1976
Rattus norvegicus
Manually annotated by BRENDA team
Foldes, A.; Meek, J.L.
Rat brain phenolsulfotransferase: partial purification and some properties
Biochim. Biophys. Acta
327
365-374
1973
Rattus norvegicus
Manually annotated by BRENDA team
Sakakibara, Y.; Takami, Y.; Zwieb, C.; Nakayama, T.; Suiko, M.; Nakamima, H.; Liu, M.C.
Purification, characterization, and molecular cloning of a novel rat liver dopa/tyrosine sulfotransferase
J. Biol. Chem.
270
30470-30478
1995
Rattus norvegicus (P52847)
Manually annotated by BRENDA team
Yang, Y.S.; Marshall, A.D.; McPhie, P.; Guo, W.X.A.; Xie, X.; Chen, X.; Jakoby, W.B.
Two phenol sulfotransferase species from one cDNA: nature of the differences
Protein Expr. Purif.
8
423-429
1996
Rattus norvegicus
Manually annotated by BRENDA team
Yang, Y.S.; Tsai, S.W.; Lin, E.S.
Effects of 3'-phosphoadenosine 5'-phosphate on the activity and folding of phenol sulfotransferase
Chem. Biol. Interact.
109
129-135
1998
Rattus norvegicus
Manually annotated by BRENDA team
Marshall, A.D.; McPhie, P.; Jakoby, W.B.
Redox control of aryl sulfotransferase specificity
Arch. Biochem. Biophys.
382
95-104
2000
Rattus norvegicus
Manually annotated by BRENDA team
Lin, E.S.; Yang, Y.S.
Nucleotide binding and sulfation catalyzed by phenol sulfotransferase
Biochem. Biophys. Res. Commun.
271
818-822
2000
Rattus norvegicus
Manually annotated by BRENDA team
Honma, W.; Kamiyama, Y.; Yoshinari, K.; Sasano, H.; Shimada, M.; Nagata, K.; Yamazoe, Y.
Enzymatic characterization and interspecies difference of phenol sulfotransferases, ST1A forms
Drug Metab. Dispos.
29
274-281
2001
Homo sapiens, Mus musculus, Mus musculus BALB/c, Mus musculus ST1a4, Oryctolagus cuniculus (Q9XT99), Oryctolagus cuniculus, Oryctolagus cuniculus ST1A8 (Q9XT99), Rattus norvegicus
Manually annotated by BRENDA team
Duffel, M.W.; Marshall, A.D.; McPhie, P.; Sharma, V.; Jakoby, W.B.
Enzymatic aspects of the phenol (aryl) sulfotransferases
Drug Metab. Rev.
33
369-395
2001
Bos taurus, Homo sapiens, Homo sapiens (O00338), Homo sapiens (O43704), Homo sapiens (O75897), Mus musculus, Mus musculus (Q3UZZ6), Mus musculus (Q80VR3), Mus musculus (Q9QWG7), Rattus norvegicus, Rattus norvegicus (P50237), Rattus norvegicus (P52847), Rattus norvegicus (Q9WUW8), Rattus norvegicus (Q9WUW9), Oryctolagus cuniculus (O46503), Oryctolagus cuniculus (Q9XT99), Macaca fascicularis (P52846), Canis lupus familiaris (Q29476), Oryctolagus cuniculus ST1A8 (Q9XT99), Oryctolagus cuniculus ST1C5 (O46503), Mus musculus ST1c4 (Q80VR3), Macaca fascicularis ST1A9 (P52846), Mus musculus ST1a4
Manually annotated by BRENDA team
Sohlenius-Sternbeck, A.K.; Orzechowski, A.
Characterization of the rates of testosterone metabolism to various products and of glutathione transferase and sulfotransferase activities in rat intestine and comparison to the corresponding hepatic and renal drug-metabolizing enzymes
Chem. Biol. Interact.
148
49-56
2004
Rattus norvegicus
Manually annotated by BRENDA team
Stakhiv, T.M.; Mesia-Vela, S.; Kauffman, F.C.
Phase II antioxidant enzyme activities in brain of male and female ACI rats treated chronically with estradiol
Brain Res.
1104
80-91
2006
Rattus norvegicus (P17988)
Manually annotated by BRENDA team
Purchartova, K.; Engels, L.; Marhol, P.; Sulc, M.; Kuzma, M.; Slamova, K.; Elling, L.; Kren, V.
Enzymatic preparation of silybin phase II metabolites: sulfation using aryl sulfotransferase from rat liver
Appl. Microbiol. Biotechnol.
97
10391-10398
2013
Rattus norvegicus (P17988)
Manually annotated by BRENDA team
Dammanahalli, J.K.; Duffel, M.W.
Oxidative modification of rat sulfotransferase 1A1 activity in hepatic tissue slices correlates with effects on the purified enzyme
Drug Metab. Dispos.
40
298-303
2012
Rattus norvegicus
Manually annotated by BRENDA team
Ro, J.; Kim, H.; Shim, B.; Kim, I.; Kim, J.; Kim, H.; Cho, J.; Khadka, P.; Yun, G.; Park, K.; Park, Y.; Lee, K.; HanI, J.; Lee, J.
In vitro metabolic modulation of aryl sulfotransferases by pharmaceutical excipients
Bull. Korean Chem. Soc.
35
2577-2580
2014
Rattus norvegicus
-
Manually annotated by BRENDA team
Purchartova, K.; Valentova, K.; Pelantova, H.; Marhol, P.; Cvacka, J.; Havlicek, L.; Krenkova, A.; Vavrikova, E.; Biedermann, D.; Chambers, C.; Kren, V.
Prokaryotic and eukaryotic aryl sulfotransferases: sulfation of quercetin and its derivatives
ChemCatChem
7
3152-3162
2015
Desulfitobacterium hafniense, Rattus norvegicus
-
Manually annotated by BRENDA team
Zhou, Z.; Li, Q.; Huang, H.; Wang, H.; Wang, Y.; Du, G.; Chen, J.; Kang, Z.
A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans
Biotechnol. Bioeng.
115
1561-1570
2018
Rattus norvegicus (P17988)
Manually annotated by BRENDA team
Asai, Y.; Sakakibara, Y.; Kondo, M.; Nadai, M.; Katoh, M.
Expression and activities of sulfotransferase in rat brain
Xenobiotica
49
270-275
2019
Rattus norvegicus
Manually annotated by BRENDA team