We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet. The first reaction produces 5'-deoxyadenosine and 4,5-secobiotin. Reaction with another equivalent of AdoMet results in abstraction of the C-6 methylene pro-S hydrogen atom from 4,5-secobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin tetrahydrothiophene ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis.
The taxonomic range for the selected organisms is: Arabidopsis thaliana The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
sulfur-(sulfur carrier)
+
2
+
2
=
+
(sulfur carrier)
+
2
+
2
+
2
Synonyms
biotin synthase, biotin synthetase, ncgl0071,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced)
-
-
-
-
biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2)
-
-
-
-
biotin synthase (Treponema pallidum gene TP0228)
-
-
-
-
biotin synthetase
-
-
-
-
biotin synthetase (Aquifex aeolicus gene bioB)
-
-
-
-
biotin synthetase (Arabidopsis thaliana clone pMB101 gene BIO2 reduced)
-
-
-
-
biotin synthetase (Bacillus subtilis gene bioB)
-
-
-
-
biotin synthetase (Chlamydia trachomatis gene birA)
-
-
-
-
GenBank AE000716-derived protein GI 2983482
-
-
-
-
GenBank AE001204-derived protein GI 3322497
-
-
-
-
GenBank AE001343-derived protein GI 3329182
-
-
-
-
GenBank AF008220-derived protein GI 2293187
-
-
-
-
GenBank U24147-derived protein
-
-
-
-
GenBank U31806-derived protein GI 1403662
-
-
-
-
GenBank U51869-derived protein GI 1277029
-
-
-
-
GenBank Z99119-derived protein GI 2635504
-
-
-
-
synthetase, biotin
-
-
-
-
synthetase, biotin (Arabidopsis thaliana clone lambdaBIO2 gene bioB)
-
-
-
-
synthetase, biotin (Arabidopsis thaliana clone pMP101 gene BIO2 reduced)
-
-
-
-
synthetase, biotin (Arabidopsis thaliana clone pYESCBS1 gene bioB)
-
-
-
-
synthetase, biotin (Bacillus subtilis clone pBIO100/pBIO350/pBIO201 gene bioB)
-
-
-
-
synthetase, biotin (Bacillus subtilis gene bioB)
-
-
-
-
synthetase, biotin (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2 reduced)
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sulfur atom transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dethiobiotin:sulfur-(sulfur carrier) sulfurtransferase
The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet. The first reaction produces 5'-deoxyadenosine and 4,5-secobiotin. Reaction with another equivalent of AdoMet results in abstraction of the C-6 methylene pro-S hydrogen atom from 4,5-secobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin tetrahydrothiophene ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
153554-27-9
synthetase, biotin (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2 reduced) /biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2)
174764-24-0
synthase, biotin (Arabidopsis thaliana clone lambdaBIO2 gene bioB) /BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced) /genBank U24147-derived protein /genBank U31806-derived protein GI 1403662 /synthetase, biotin (Arabidopsis thaliana clone pMP101 gene BIO2 reduced) /synthetase, biotin (Arabidopsis thaliana clone pYESCBS1 gene bioB)
179608-56-1
synthetase, biotin (Bacillus subtilis clone pBIO100/pBIO350/pBIO201 gene bioB) /biotin synthetase (Bacillus subtilis gene bioB) /genBank AF008220-derived protein GI 2293187 /genBank U51869-derived protein GI 1277029 /genBank Z99119-derived protein GI 2635504 /Synthetase, biotin (Bacillus subtilis gene bioB)
204794-88-7
biotin synthetase (Aquifex aeolicus gene bioB) /genBank AE000716-derived protein GI 2983482
209603-31-6
biotin synthase (Treponema pallidum gene TP0228) /genBank AE001204-derived protein GI 3322497
215108-34-2
biotin synthetase (Chlamydia trachomatis gene birA) /genBank AE001343-derived protein GI 3329182
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dethiobiotin + sulfur
biotin
-
-
-
?
dethiobiotin + sulfur
biotin
dethiobiotin + sulfur
biotin
-
-
-
?
dethiobiotin + sulfur
biotin
-
-
-
?
dethiobiotin + sulfur
biotin
-
-
-
?
dethiobiotin + sulfur
biotin
-
-
-
?
dethiobiotin + sulfur
biotin
-
expression of the BIO2 gene appears to be induced under biotin-limiting conditions
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dethiobiotin + sulfur
biotin
-
expression of the BIO2 gene appears to be induced under biotin-limiting conditions
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acidomycin
-
structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-fructose 1,6-bisphosphate
-
enhances biotin formation
dithiothreitol
-
required
L-cysteine
-
highly stimulates
S-adenosyl-L-methionine
-
required
additional information
-
one or more unidentified factors from mitochoncrial matrix of pea and potato and from mitochondrial membranes of pea, in addition to the purified enzyme, are obligatory for the conversion of dithiobiotin to biotin in plants
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.03
dethiobiotin
-
pH 8, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00055
dethiobiotin
-
pH 8, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MFDR_ARATH
483
0
53070
Swiss-Prot
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
50000
AdxR isozyme, SDS-PAGE
22000
Adx1 isozyme, SDS-PAGE
41650
-
calculated from nucleotide sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli. The amino-terminal portion of the BIO2 gene may play a role in localizing the BIO2 protein to a subcellular compartment
-
the primary sequence of the Arabidopsis biotin synthase is similar to biotin synthase from Escherichia coli, Serratia marcescens and Saccharomyces cerevisiae, about 50% sequence identity, and more distantly related to the Bacillus sphaericus enzyme, 33% sequence identity
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Weaver, L.M.; Yu, F.; Wurtele, E.S.; Nikolau, B.J.
Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana
Plant Physiol.
110
1021-1028
1996
Arabidopsis thaliana
brenda
Patton, D.A.; Jonson, M.; Ward, E.R.
Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression
Plant Physiol.
112
371-378
1996
Arabidopsis thaliana
brenda
Picciocchi, A.; Douce, R.; Alban, C.
Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis
Plant Physiol.
127
1224-1233
2001
Arabidopsis thaliana
brenda
Picciocchi, A.; Douce, R.; Alban, C.
The plant biotin synthase reaction. Identification and characterization of essential mitochondrial accessory protein components
J. Biol. Chem.
278
24966-24975
2003
Arabidopsis thaliana (O49543), Arabidopsis thaliana (Q8S904), Arabidopsis thaliana (Q8W3L1), Arabidopsis thaliana (Q9M0V0), Arabidopsis thaliana
brenda