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Information on EC 2.8.1.6 - biotin synthase and Organism(s) Arabidopsis thaliana and UniProt Accession O49543

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.1 Sulfurtransferases
                2.8.1.6 biotin synthase
IUBMB Comments
The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet. The first reaction produces 5'-deoxyadenosine and 4,5-secobiotin. Reaction with another equivalent of AdoMet results in abstraction of the C-6 methylene pro-S hydrogen atom from 4,5-secobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin tetrahydrothiophene ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis.
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Arabidopsis thaliana
UNIPROT: O49543
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
biotin synthase, biotin synthetase, ncgl0071, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced)
-
-
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BioB
-
-
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biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2)
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-
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biotin synthase (Treponema pallidum gene TP0228)
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-
-
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biotin synthetase
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-
-
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biotin synthetase (Aquifex aeolicus gene bioB)
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-
-
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biotin synthetase (Arabidopsis thaliana clone pMB101 gene BIO2 reduced)
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-
-
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biotin synthetase (Bacillus subtilis gene bioB)
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-
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biotin synthetase (Chlamydia trachomatis gene birA)
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-
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GenBank AE000716-derived protein GI 2983482
-
-
-
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GenBank AE001204-derived protein GI 3322497
-
-
-
-
GenBank AE001343-derived protein GI 3329182
-
-
-
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GenBank AF008220-derived protein GI 2293187
-
-
-
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GenBank U24147-derived protein
-
-
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GenBank U31806-derived protein GI 1403662
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-
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GenBank U51869-derived protein GI 1277029
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-
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GenBank Z99119-derived protein GI 2635504
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-
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synthetase, biotin
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synthetase, biotin (Arabidopsis thaliana clone lambdaBIO2 gene bioB)
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-
-
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synthetase, biotin (Arabidopsis thaliana clone pMP101 gene BIO2 reduced)
-
-
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synthetase, biotin (Arabidopsis thaliana clone pYESCBS1 gene bioB)
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-
-
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synthetase, biotin (Bacillus subtilis clone pBIO100/pBIO350/pBIO201 gene bioB)
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-
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synthetase, biotin (Bacillus subtilis gene bioB)
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-
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synthetase, biotin (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2 reduced)
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfur atom transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
dethiobiotin:sulfur-(sulfur carrier) sulfurtransferase
The enzyme binds a [4Fe-4S] and a [2Fe-2S] cluster. In every reaction cycle, the enzyme consumes two molecules of AdoMet. The first reaction produces 5'-deoxyadenosine and 4,5-secobiotin. Reaction with another equivalent of AdoMet results in abstraction of the C-6 methylene pro-S hydrogen atom from 4,5-secobiotin, and the resulting carbon radical is quenched via formation of an intramolecular C-S bond, thus closing the biotin tetrahydrothiophene ring. The sulfur donor is believed to be the [2Fe-2S] cluster, which is sacrificed in the process, so that in vitro the reaction is a single turnover. In vivo, the [2Fe-2S] cluster can be reassembled by the Isc or Suf iron-sulfur cluster assembly systems, to allow further catalysis.
CAS REGISTRY NUMBER
COMMENTARY hide
153554-27-9
synthetase, biotin (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2 reduced) /biotin synthase (Saccharomyces cerevisiae strain 20B-12 clone pUCH2.4 gene BIO2)
174764-24-0
synthase, biotin (Arabidopsis thaliana clone lambdaBIO2 gene bioB) /BIO2 protein (Arabidopsis thaliana clone pMP101 gene BIO2 reduced) /genBank U24147-derived protein /genBank U31806-derived protein GI 1403662 /synthetase, biotin (Arabidopsis thaliana clone pMP101 gene BIO2 reduced) /synthetase, biotin (Arabidopsis thaliana clone pYESCBS1 gene bioB)
179608-56-1
synthetase, biotin (Bacillus subtilis clone pBIO100/pBIO350/pBIO201 gene bioB) /biotin synthetase (Bacillus subtilis gene bioB) /genBank AF008220-derived protein GI 2293187 /genBank U51869-derived protein GI 1277029 /genBank Z99119-derived protein GI 2635504 /Synthetase, biotin (Bacillus subtilis gene bioB)
204794-88-7
biotin synthetase (Aquifex aeolicus gene bioB) /genBank AE000716-derived protein GI 2983482
209603-31-6
biotin synthase (Treponema pallidum gene TP0228) /genBank AE001204-derived protein GI 3322497
215108-34-2
biotin synthetase (Chlamydia trachomatis gene birA) /genBank AE001343-derived protein GI 3329182
80146-93-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dethiobiotin + sulfur
biotin
show the reaction diagram
-
-
-
?
dethiobiotin + sulfur
biotin
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dethiobiotin + sulfur
biotin
show the reaction diagram
-
expression of the BIO2 gene appears to be induced under biotin-limiting conditions
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
highly stimulates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acidomycin
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structural analog of biotin, complete inhibition at 0.4 mM, 50% inhibition at 0.035 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose 1,6-bisphosphate
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enhances biotin formation
dithiothreitol
-
required
L-cysteine
-
highly stimulates
S-adenosyl-L-methionine
-
required
additional information
-
one or more unidentified factors from mitochoncrial matrix of pea and potato and from mitochondrial membranes of pea, in addition to the purified enzyme, are obligatory for the conversion of dithiobiotin to biotin in plants
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
dethiobiotin
-
pH 8, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00055
dethiobiotin
-
pH 8, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MNIF1_ARATH
453
0
50296
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22000
Adx1 isozyme, SDS-PAGE
41650
-
calculated from nucleotide sequence
50000
AdxR isozyme, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli. The amino-terminal portion of the BIO2 gene may play a role in localizing the BIO2 protein to a subcellular compartment
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the primary sequence of the Arabidopsis biotin synthase is similar to biotin synthase from Escherichia coli, Serratia marcescens and Saccharomyces cerevisiae, about 50% sequence identity, and more distantly related to the Bacillus sphaericus enzyme, 33% sequence identity
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Weaver, L.M.; Yu, F.; Wurtele, E.S.; Nikolau, B.J.
Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana
Plant Physiol.
110
1021-1028
1996
Arabidopsis thaliana
Manually annotated by BRENDA team
Patton, D.A.; Jonson, M.; Ward, E.R.
Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression
Plant Physiol.
112
371-378
1996
Arabidopsis thaliana
Manually annotated by BRENDA team
Picciocchi, A.; Douce, R.; Alban, C.
Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis
Plant Physiol.
127
1224-1233
2001
Arabidopsis thaliana
Manually annotated by BRENDA team
Picciocchi, A.; Douce, R.; Alban, C.
The plant biotin synthase reaction. Identification and characterization of essential mitochondrial accessory protein components
J. Biol. Chem.
278
24966-24975
2003
Arabidopsis thaliana (O49543), Arabidopsis thaliana (Q8S904), Arabidopsis thaliana (Q8W3L1), Arabidopsis thaliana (Q9M0V0), Arabidopsis thaliana
Manually annotated by BRENDA team