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Information on EC 2.8.1.13 - tRNA-uridine 2-sulfurtransferase and Organism(s) Bacillus subtilis and UniProt Accession O35020

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.1 Sulfurtransferases
                2.8.1.13 tRNA-uridine 2-sulfurtransferase
IUBMB Comments
The enzyme, found in bacteria, catalyses formation of the 2-thiouridine modification in the wobble position of tRNAGln, tRNALys and tRNAGlu.
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This record set is specific for:
Bacillus subtilis
UNIPROT: O35020
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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
a [protein]-S-sulfanyl-L-cysteine
+
uacil34 in tRNA
+
+
=
a [protein]-L-cysteine
+
2-thiouracil34 in tRNA
+
+
+
Synonyms
MnmA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
[protein]-S-sulfanyl-L-cysteine:tRNA (uridine34-2-O)-sulfurtransferase
The enzyme, found in bacteria, catalyses formation of the 2-thiouridine modification in the wobble position of tRNAGln, tRNALys and tRNAGlu.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor
a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor
show the reaction diagram
-
enzyme adenylates and subsequently thiolates tRNA to form the s2U modification
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
in a conditional Bacillus subtilis knockout strain s2U abundance correlates with tRNA-uridine 2-sulfurtransferase MnmA expression, and MnmA is able to complement Escherichia coli MnmA-deficient strains. Cysteine desulfurase IscS is able to transfer sulfur directly to MnmA in presence of ATP. Both proteins are sufficient for s2U biosynthesis in a pathway independent of the one used in Escherichia coli
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C104A
mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo
C200A
mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo
C51A
mutation completely hinders the ability of MnmA along with YrvO to synthesize s2U in vivo
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Black, K.A.; Dos Santos, P.C.
Abbreviated pathway for biosynthesis of 2-thiouridine in Bacillus subtilis
J. Bacteriol.
197
1952-1962
2015
Bacillus subtilis (O35020), Bacillus subtilis 168 (O35020)
Manually annotated by BRENDA team