Information on EC 2.8.1.1 - thiosulfate sulfurtransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

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EC NUMBER
COMMENTARY hide
2.8.1.1
-
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RECOMMENDED NAME
GeneOntology No.
thiosulfate sulfurtransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiosulfate + cyanide = sulfite + thiocyanate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfur atom transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfide oxidation IV (metazoa)
-
-
thiosulfate disproportionation IV (rhodanese)
-
-
non-pathway related
-
-
Sulfur metabolism
-
-
Microbial metabolism in diverse environments
-
-
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SYSTEMATIC NAME
IUBMB Comments
thiosulfate:cyanide sulfurtransferase
A few other sulfur compounds can act as donors.
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-04-4
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
cabbage
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
vervet monkey
-
-
Manually annotated by BRENDA team
Columbia domesticus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
comparison of enzyme activity with several tissues of sheep, cattle, goat, camel, dog, chicken and human
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
chinese hamster
-
-
Manually annotated by BRENDA team
parsley
-
-
Manually annotated by BRENDA team
serovar typhimurium
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains: O90, O77, O10, O82, O80
-
-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-(dimethylamino)-4'-azobenzene sulfinate + thiosulfate
4-(dimethylamino)-4'-azobenzene thiosulfonate + SO32-
show the reaction diagram
-
-
-
-
?
4-(dimethylamino)-4'-azobenzene thiosulfonate + cyanide
4-(dimethylamino)-4'-azobenzene sulfinate + SCN-
show the reaction diagram
-
-
-
-
?
4-(dimethylamino)-4'-azobenzene thiosulfonate + glutathione
4-(dimethylamino)-4'-azobenzene sulfinate + reduced glutathione
show the reaction diagram
-
-
-
-
?
5-dimethylamino-1-naphthalene sulfinate + thiosulfate
5-dimethylamino-1-naphthalene thiosulfonate + SO32-
show the reaction diagram
-
-
-
-
?
5-dimethylamino-1-naphthalene thiosulfonate + cyanide
5-dimethylamino-1-naphthalene sulfinate + SCN-
show the reaction diagram
-
-
-
-
?
5-dimethylamino-1-naphthalene thiosulfonate + glutathione
5-dimethylamino-1-naphthalene sulfinate + reduced glutathione
show the reaction diagram
-
-
-
-
?
alkyl sulfinate + cyanide
?
show the reaction diagram
aryl sulfinate + cyanide
?
show the reaction diagram
cyanide + 2-aminoethanethiosulfate
?
show the reaction diagram
-
K249A
-
-
?
cyanide + 3-mercaptopyruvate
?
show the reaction diagram
-
-
-
?
cyanide + ethanethiosulfate
?
show the reaction diagram
-
K249A
-
-
?
cyanide + p-toluenethiosulfate
?
show the reaction diagram
-
K249A
-
-
?
cysteine + cyanide
cyanoalanine + H2S
show the reaction diagram
H2S + cyanide
?
show the reaction diagram
-
very poor substrate
-
-
?
H2S + cyanide
thiocyanate
show the reaction diagram
persulfide + cyanide
?
show the reaction diagram
-
-
-
-
?
polysulfide + cyanide
? + thiocyanate
show the reaction diagram
-
-
-
-
?
reduced thioredoxin + methane thiosulfonate
?
show the reaction diagram
-
only the less negative rhodanese isoform catalyzes the oxidation of thioredoxin
-
-
r
tetrathionate + cyanide
? + thiocyanate
show the reaction diagram
-
-
-
-
?
thiocystine + cyanide
?
show the reaction diagram
thioredoxin + thiosulfate
?
show the reaction diagram
-
-
-
-
?
thiosulfate + 2-mercaptoethanol
?
show the reaction diagram
-
20% of the activity with cyanide
-
-
?
thiosulfate + a thiol
?
show the reaction diagram
-
single displacement mechanism
-
-
?
thiosulfate + benzene thiosulfonate
?
show the reaction diagram
-
-
-
-
?
thiosulfate + borohydride
?
show the reaction diagram
thiosulfate + cyanide
sulfite + thiocyanate
show the reaction diagram
thiosulfate + D,L-homocysteine
?
show the reaction diagram
-
19% of the activity with cyanide
-
-
?
thiosulfate + dihydrolipoate
?
show the reaction diagram
thiosulfate + dithionite
?
show the reaction diagram
thiosulfate + dithiothreitol
?
show the reaction diagram
-
-
-
-
?
thiosulfate + glutathione
?
show the reaction diagram
-
20% of the activity with cyanide
-
-
?
thiosulfate + L-cysteine
?
show the reaction diagram
-
12% of the activity with cyanide
-
-
?
thiosulfate + monothiol
?
show the reaction diagram
-
-
-
-
?
thiosulfate + N-acetyl-L-cysteine
?
show the reaction diagram
-
5% of the activity with cyanide
-
-
?
thiosulfate + sulfite
sulfite + thiosulfate
show the reaction diagram
thiosulfate + thiosulfinate
?
show the reaction diagram
-
-
-
-
?
thiosulfonate + cyanide
?
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiosulfate + cyanide
sulfite + thiocyanate
show the reaction diagram
additional information
?
-
-
enzyme does not metabolize sulfide. The rate limiting step in sulfide detoxification is oxidation by a sulfide oxidase to thiosulfate
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
one ion per monomer, but not necessary for enzyme activity
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
2-Naphthalene sulfonate
-
-
2-oxoglutarate
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
Anions
-
CaCl2
Cd2+
-
more than 70% inhibition at 0.1 mM
Cu2+
-
more than 70% inhibition at 0.1 mM
Dinitrobenzene
-
-
-
Dinitrofluorobenzene
-
-
-
dithioerythritol
dithiothreitol
DL-dihydrolipoate
DL-isocitrate
-
-
E-4-ethyl-2-[E-hydroxyimino]-5-nitro-3-hexenamide
-
dithithreitol prevents enzyme inhibition and fully restores enzyme activity
fumarate
-
-
glutathione
-
activation of the enzyme with a maximum at 0.005-0.02 mM, inhibition above
H2O2
-
the less negative isoform is more instable to H2O2
iodoacetamide
-
complete inhibition at 20 mM
iodoacetate
L-cysteine
lipoate
menadione
-
1 h incubation at 0.02 mM leads to loss of the activity of thiosulfate sulfurtransferase by 33%. In addition, 3-mercaptosulfurtransferase and the level of sulfane sulfur and glutathione are decreased
N-bromosuccinimide
-
-
Ni2+
-
more than 70% inhibition at 0.1 mM
oxaloacetate
-
-
p-chloromercuribenzoate
Phenylglyoxal
-
-
pyruvate
-
-
S-nitroso-glutathione
-
dithiothreitol prevents enzyme inhibition and fully restores enzyme activity
S-nitroso-N-acetyl-penicillamine
-
dithithreitol prevents enzyme inhibition and fully restores enzyme activity
SO42-
-
incubation of mitochondria with sulfate causes a significant decrease in activity
Sodium arsenite
-
-
Sodium borohydride
-
pH 8.0
succinate
-
-
sulfhydryl reagents
-
-
Sulfide
-
activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29
sulfite
Thiocyanate
-
-
thiosulfate
Triton X-100
-
slight
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Butyrate
-
significantly increase of enzyme activity and expression in human colon cancer cell line HT-29
dihydrolipoate
-
increases activity at low concentrations
dithioerythritol
-
maximal activation at 0.005 mM, inhibition above
glutathione
-
increases activity at low concentrations
L-cysteine
reduced glutathione
-
enhances activity
Sulfide
-
activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29
sulfite
-
activating effect at high thiosulfate concentrations in p-dimethylaminoazobenzene-treated mice
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7 - 51.7
3-Mercaptopyruvate
0.03 - 27
CN-
1.7 - 14
cyanide
8.8
H2S
-
20°C, pH 9.2
0.05
Polysulfide
-
pH 9.0, 80°C
-
6.9
tetrathionate
-
pH 9.0, 80°C
0.034
thioredoxin
-
2°C, pH 8.6
0.53 - 603
thiosulfate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7 - 54
3-Mercaptopyruvate
2.8 - 400
thiosulfate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
intestine
0.008
-
abomasum
0.012
-
enzyme from muscle, pH 9.2, 37°C
0.014
-
intestine
0.015
-
with H2S as substrate
0.016
-
omasum
0.017
-
spleen
0.018
-
rumen
0.026
-
abomasum
0.029
-
rumen
0.03
-
enzyme from lung, pH 9.2, 37°C
0.033
-
rumen
0.035
-
enzyme from brain, pH 9.2, 37°C
0.047
-
oesophagus
0.069
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine
0.071
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 0.9% NaCl as control; rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiosulfate
0.077
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine
0.078
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine
0.085
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid
0.092
-
kidney
0.1
-
microsomal rhodanese 28 days after common bile duct ligation
0.11
-
rhodanese activity in muscle of omasum of male and female animals
0.113
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid
0.128
-
stomach
0.158
-
small intestine
0.159
-
microsomal rhodanese 28 days after common bile duct ligation
0.183
-
liver
0.19
-
rhodanese activity in muscle of reticulum of rumen of female animals
0.21
-
rhodanese activity in fundus muscle of abomasum of male animals
0.214
-
liver
0.22
-
rhodanese activity in fundus muscle of abomasum of female animals
0.23
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine
0.246
-
left colon
0.254
-
rectum
0.289
-
microsomal rhodanese 28 days after apparent operation
0.29
-
rhodanese activity in small intestine of female animals
0.292
-
right colon
0.3
-
rhodanese activity in heart ventricle of female animals
0.314
-
liver
0.34
-
rhodanese activity in heart ventricle of male animals
0.362
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid
0.374
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid
0.376
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine
0.38
-
rhodanese activity in heart atrium of male animals
0.39
-
rhodanese activity in heart atrium of female animals
0.4
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine
0.426
-
kidney
0.476
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiazolidine-4-carboxylic acid
0.48
-
enzyme from brain, pH 9.2, 37°C
0.49
-
rhodanese activity in brain of female animals
0.51
-
rhodanese activity in fundus epithelium of abomasum of male animals
0.52
-
rhodanese activity in fundus epithelium of abomasum of female animals
0.57
-
rhodanese activity in pylore epithelium of abomasum of female animals
0.59
-
muscle
0.6
-
rhodanese activity in large intestine of male animals
0.619
-
kidney
0.62
-
rhodanese activity in large intestine of male animals
0.63
-
preparative PAGE
0.67
-
spleen
0.82
-
enzyme from kidney cortex, pH 9.2, 37°C
0.88
-
kidney
0.903
-
liver
0.965
-
enzyme from kidney, pH 9.2, 37°C
0.97
-
rhodanese activity in kidney cortex of female animals
1.03
-
cytosolic rhodanese 42 days after common bile duct ligation
1.06
-
cytosolic rhodanese 28 days after common bile duct ligation
1.08
-
mitochondrial rhodanese 42 days after common bile duct ligation
1.11
-
cytosolic rhodanese 14 days after common bile duct ligation
1.14
-
mitochondrial rhodanese 28 days after common bile duct ligation
1.25
-
liver
1.35
-
cytosolic rhodanese 28 days after common bile duct ligation
1.4
-
enzyme from kidney, pH 9.2, 37°C
1.68
-
mitochondrial rhodanese 28 days after common bile duct ligation
1.83
-
cytosolic rhodanese 7 days after common bile duct ligation
2.18
-
mitochondrial rhodanese 14 days after common bile duct ligation
2.27
-
cytosolic rhodanese 3 days after common bile duct ligation
2.44
-
mitochondrial rhodanese 7 days after common bile duct ligation
2.5
-
cytosolic rhodanese 2 days after common bile duct ligation
2.61
-
cytosolic rhodanese 1 day after common bile duct ligation
2.85
-
cytosolic rhodanese 12 h after common bile duct ligation
2.99
-
rhodanese activity in omasum of male animals
3
-
rhodanese activity in liver of female animals; rhodanese activity in omasum of female animals
3.06
-
cytosolic rhodanese 12 h after apparent operation
3.07
-
cytosolic rhodanese 14 and 42 days after apparent operation
3.08
-
cytosolic rhodanese 3 days after apparent operation
3.09
-
cytosolic rhodanese 7 and 28 days after apparent operation
3.1
-
cytosolic rhodanese 1 and 2 days after apparent operation
3.14
-
rhodanese activity in reticulum of rumen of female animals
3.25
-
mitochondrial rhodanese 3 days after common bile duct ligation
3.63
-
cytosolic rhodanese 28 days after apparent operation
3.82
-
rhodanese activity in epithelium of omasum of female animals
4.02
-
mitochondrial rhodanese 1 day after common bile duct ligation
4.16
-
rhodanese activity in rumen of male animals
4.18
-
mitochondrial rhodanese 12 h after common bile duct ligation
4.23
-
rhodanese activity in rumen of female animals
4.25
-
mitochondrial rhodanese 7 and 42 days after apparent operation
4.26
-
mitochondrial rhodanese 14 and 28 days after apparent operation
4.3
-
mitochondrial rhodanese 2 and 3 days after apparent operation
4.31
-
mitochondrial rhodanese 1 day after apparent operation
4.35
-
mitochondrial rhodanese 12 h after apparent operation
4.64
-
mitochondrial rhodanese 28 days after apparent operation
4.95
-
enzyme from liver, pH 9.2, 37°C
5
-
rhodanese activity in epithelium of reticulum of rumen of female animals
5.08
-
rhodanese activity in epithelium of reticulum of rumen of male animals
5.21
-
enzyme from liver, pH 9.2, 37°C
8.92
-
rhodanese activity in epithelium of rumen of female animals
8.99
-
rhodanese activity in epithelium of rumen of male animals
10.3
-
enzyme from epithelial layer of rumen, pH 9.2, 37°C
15.9
-
Sephadex G-100
16.3
-
enzyme from epithelial layer of rumen, pH 9.2, 37°C
70
-
QAE-Sephadex column II
85.3
-
substrate thiosulfate, pH 9.2, 22°C
225
-
recombinant wild-type enzyme
332
-
wild-type
353
-
Ser-1
389
-
Ser-2
391
-
one additional Arg residue at the 3'-end followed by a TAA stop codon
491
-
C-terminally extended mutant enzyme
575.5
-
R186L
600
-
main fraction of Sephadex G-200 gel chromatography
672.8
-
wild-type
700
-
preparative polyacrylamide gel electrophoresis
720
-
-
730
-
purificated liver rhodanese
818
-
recombinant wild-type
850
-
pH 9.0, 80°C
1071
-
after Sephacryl S-200-HR gel filtration
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
mitochondria
7 - 8.5
-
-
7.5 - 8.5
-
-
8.5 - 10.5
-
strain O90 and strain O77
8.5 - 11.5
-
strain O10
10.5
-
strain O82
11.5
-
strain O80
additional information
-
cytoplasmic enzyme exhibits a more acidic pH-optimum than the mitochondrial enzyme
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10.5
-
pH 5.5: about 45% of maximal activity, pH 10.5: about 95% of maximal activity, pH 11.0: about 15% of maximal activity
6 - 11
-
pH 6.0: about 75% of activity maximum, pH 11.0: about 70% of activity maximum
7
-
no residual activity below
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 32
35 - 55
-
strain O90
45
-
strain O10 and strain O82
73
-
mitochondrial and cytosolic enzyme of untreated mice
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 50
-
-
25
-
8% of maximum activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectrophoresis in polyacrylamide gels, cytosol
5.3
-
isoelectrophoresis in polyacrylamide gels, mitochondria
6.6
-
isoelectric focusing in Phast Gel isoelectric focusing gel with pH range: 3 - 9
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
atrium and ventricle
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
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