Information on EC 2.8.1.1 - thiosulfate sulfurtransferase

NEM

Ni2+

more than 70% inhibition at 0.1 mM

oxaloacetate

645511

p-chloromercuribenzoate

Phenylglyoxal

pyruvate

S-nitroso-glutathione

dithiothreitol prevents enzyme inhibition and fully restores enzyme activity

645521

S-nitroso-N-acetyl-penicillamine

dithithreitol prevents enzyme inhibition and fully restores enzyme activity

645521

SDS

SO32-

19 entries

SO42-

incubation of mitochondria with sulfate causes a significant decrease in activity

Sodium arsenite

Sodium borohydride

pH 8.0

succinate

sulfhydryl reagents

Sulfide

activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29

sulfite

Thiocyanate

thiosulfate

Triton X-100

slight

Zn2+

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2-mercaptoethanol

Butyrate

Homo sapiens

significantly increase of enzyme activity and expression in human colon cancer cell line HT-29

671241

dihydrolipoate

increases activity at low concentrations

dithioerythritol

maximal activation at 0.005 mM, inhibition above

glutathione

increases activity at low concentrations

L-cysteine

reduced glutathione

enhances activity

Sulfide

Homo sapiens

activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29

671241

sulfite

activating effect at high thiosulfate concentrations in p-dimethylaminoazobenzene-treated mice

645539

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3.7 - 51.7

3-Mercaptopyruvate

0.03 - 27

CN-

19 entries

1.7 - 14

cyanide

4 entries

8.8

H2S

Homo sapiens

20°C, pH 9.2

645535

0.05

Polysulfide

pH 9.0, 80°C

6.9

tetrathionate

pH 9.0, 80°C

0.034

thioredoxin

Escherichia coli

2°C, pH 8.6

645532

0.53 - 603

thiosulfate

43 entries

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0.7 - 54

3-Mercaptopyruvate

2.8 - 400

thiosulfate

6 entries

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0.003

intestine

0.008

abomasum

0.01

0.012

enzyme from muscle, pH 9.2, 37°C

0.014

intestine

0.015

with H2S as substrate

0.016

omasum

0.017

spleen

0.018

rumen

0.02

0.026

abomasum

0.027

0.029

rumen

0.03

enzyme from lung, pH 9.2, 37°C

0.033

rumen

0.035

enzyme from brain, pH 9.2, 37°C

0.04

0.046

0.047

oesophagus

0.05

0.06

0.069

rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine

0.07

0.071

rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 0.9% NaCl as control; rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiosulfate

0.077

rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine

0.078

rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine

0.08

4 entries

0.085

rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid

0.09

0.092

kidney

0.1

microsomal rhodanese 28 days after common bile duct ligation

0.11

rhodanese activity in muscle of omasum of male and female animals

0.113

rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid

0.12

0.128

stomach

0.13

0.15

0.158

small intestine

0.159

microsomal rhodanese 28 days after common bile duct ligation

0.18

0.183

liver

0.19

rhodanese activity in muscle of reticulum of rumen of female animals

0.21

rhodanese activity in fundus muscle of abomasum of male animals

0.214

liver

0.22

rhodanese activity in fundus muscle of abomasum of female animals

0.23

rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine

0.24

0.246

left colon

0.25

0.254

rectum

0.26

0.28

0.289

microsomal rhodanese 28 days after apparent operation

0.29

rhodanese activity in small intestine of female animals

0.292

right colon

0.3

rhodanese activity in heart ventricle of female animals

0.31

0.314

liver

0.32

0.34

rhodanese activity in heart ventricle of male animals

0.35

0.36

0.362

rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid

0.37

0.374

rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid

0.376

rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine

0.38

rhodanese activity in heart atrium of male animals

0.39

rhodanese activity in heart atrium of female animals

0.4

rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine

0.426

kidney

0.47

0.476

rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiazolidine-4-carboxylic acid

0.48

Ovis aries

enzyme from brain, pH 9.2, 37°C

0.49

rhodanese activity in brain of female animals

0.51

rhodanese activity in fundus epithelium of abomasum of male animals

0.52

rhodanese activity in fundus epithelium of abomasum of female animals

0.56

0.57

rhodanese activity in pylore epithelium of abomasum of female animals

0.59

muscle

0.6

rhodanese activity in large intestine of male animals

0.619

kidney

0.62

rhodanese activity in large intestine of male animals

0.63

preparative PAGE

0.67

spleen

0.82

enzyme from kidney cortex, pH 9.2, 37°C

0.88

kidney

0.903

liver

0.94

0.965

enzyme from kidney, pH 9.2, 37°C

0.97

rhodanese activity in kidney cortex of female animals

1.03

cytosolic rhodanese 42 days after common bile duct ligation

1.06

cytosolic rhodanese 28 days after common bile duct ligation

1.08

mitochondrial rhodanese 42 days after common bile duct ligation

1.11

cytosolic rhodanese 14 days after common bile duct ligation

1.14

mitochondrial rhodanese 28 days after common bile duct ligation

1.2

1.25

liver

1.35

cytosolic rhodanese 28 days after common bile duct ligation

1.4

Equus caballus

enzyme from kidney, pH 9.2, 37°C

1.68

mitochondrial rhodanese 28 days after common bile duct ligation

1.7

1.83

cytosolic rhodanese 7 days after common bile duct ligation

2.18

mitochondrial rhodanese 14 days after common bile duct ligation

2.27

cytosolic rhodanese 3 days after common bile duct ligation

2.44

mitochondrial rhodanese 7 days after common bile duct ligation

2.5

cytosolic rhodanese 2 days after common bile duct ligation

2.61

cytosolic rhodanese 1 day after common bile duct ligation

2.85

cytosolic rhodanese 12 h after common bile duct ligation

2.93

2.99

rhodanese activity in omasum of male animals

rhodanese activity in liver of female animals; rhodanese activity in omasum of female animals

3.06

cytosolic rhodanese 12 h after apparent operation

3.07

cytosolic rhodanese 14 and 42 days after apparent operation

3.08

cytosolic rhodanese 3 days after apparent operation

3.09

cytosolic rhodanese 7 and 28 days after apparent operation

3.1

cytosolic rhodanese 1 and 2 days after apparent operation

3.14

rhodanese activity in reticulum of rumen of female animals

3.25

mitochondrial rhodanese 3 days after common bile duct ligation

3.31

3.63

cytosolic rhodanese 28 days after apparent operation

3.76

3.82

rhodanese activity in epithelium of omasum of female animals

4.02

mitochondrial rhodanese 1 day after common bile duct ligation

4.16

rhodanese activity in rumen of male animals

4.18

mitochondrial rhodanese 12 h after common bile duct ligation

4.23

rhodanese activity in rumen of female animals

4.25

mitochondrial rhodanese 7 and 42 days after apparent operation

4.26

mitochondrial rhodanese 14 and 28 days after apparent operation

4.3

mitochondrial rhodanese 2 and 3 days after apparent operation

4.31

mitochondrial rhodanese 1 day after apparent operation

4.35

mitochondrial rhodanese 12 h after apparent operation

4.64

mitochondrial rhodanese 28 days after apparent operation

4.95

Bos taurus

enzyme from liver, pH 9.2, 37°C

rhodanese activity in epithelium of reticulum of rumen of female animals

5.08

rhodanese activity in epithelium of reticulum of rumen of male animals

5.21

Ovis aries

enzyme from liver, pH 9.2, 37°C

8.92

rhodanese activity in epithelium of rumen of female animals

8.99

rhodanese activity in epithelium of rumen of male animals

10.3

Bos taurus

enzyme from epithelial layer of rumen, pH 9.2, 37°C

15.9

Sephadex G-100

16.3

enzyme from epithelial layer of rumen, pH 9.2, 37°C

Escherichia coli

645532

QAE-Sephadex column II

85.3

Bos taurus

substrate thiosulfate, pH 9.2, 22°C

673274

225

Rhodopseudomonas palustris

recombinant wild-type enzyme

645522

242

Chlorocebus aethiops

645499

297.9

332

wild-type

353

Ser-1

389

Ser-2

391

one additional Arg residue at the 3'-end followed by a TAA stop codon

645529

440

T232A

645522

491

Oncorhynchus mykiss

C-terminally extended mutant enzyme

645531

550

T232K

575.5

R186L

600

main fraction of Sephadex G-200 gel chromatography

672.8

wild-type

700

preparative polyacrylamide gel electrophoresis

720

730

purificated liver rhodanese

645508

816

818

Oncorhynchus mykiss

recombinant wild-type

850

pH 9.0, 80°C

1071

after Sephacryl S-200-HR gel filtration

additional information

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6.5

mitochondria

7 - 8.5

7.5

7.5 - 8.5

8 - 8.5

8.5 - 10.5

strain O90 and strain O77

8.5 - 11.5

strain O10

9.4

10.5

strain O82

11.5

strain O80

additional information

cytoplasmic enzyme exhibits a more acidic pH-optimum than the mitochondrial enzyme

645538

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5.5 - 10.5

Eidolon helvum

pH 5.5: about 45% of maximal activity, pH 10.5: about 95% of maximal activity, pH 11.0: about 15% of maximal activity

662068

6 - 11

Trametes sanguinea

pH 6.0: about 75% of activity maximum, pH 11.0: about 70% of activity maximum

645513

no residual activity below

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20 - 32

35 - 55

strain O90

strain O10 and strain O82

mitochondrial and cytosolic enzyme of untreated mice

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0 - 50

8% of maximum activity

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4.7 - 4.8

645496

Rana temporaria

isoelectrophoresis in polyacrylamide gels, cytosol

5.1

5.3

isoelectrophoresis in polyacrylamide gels, mitochondria

645498

6.6

isoelectric focusing in Phast Gel isoelectric focusing gel with pH range: 3 - 9

Ehrlich ascites carcinoma cell

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atrium and ventricle

additional information

8 entries

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endoplasmic reticulum