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Information on EC 2.7.9.1 - pyruvate, phosphate dikinase and Organism(s) Flaveria bidentis and UniProt Accession Q39735

for references in articles please use BRENDA:EC2.7.9.1
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This record set is specific for:
Flaveria bidentis
UNIPROT: Q39735 not found.
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Word Map
The taxonomic range for the selected organisms is: Flaveria bidentis
The enzyme appears in selected viruses and cellular organisms
Synonyms
pyruvate orthophosphate dikinase, pyruvate phosphate dikinase, pyruvate pi dikinase, ppdk1, pyruvate-phosphate dikinase, c4-ppdk, osppdkb, cytosolic pyruvate orthophosphate dikinase, ppdk2, cyppdk, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pyruvate, orthophosphate dikinase
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-
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pyruvate, Pi dikinase
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pyruvate-inorganic phosphate dikinase
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pyruvate-phosphate dikinase
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pyruvate-phosphate dikinase (phosphorylating)
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pyruvate-phosphate ligase
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pyruvic-phosphate dikinase
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pyruvic-phosphate ligase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
ATP:pyruvate, phosphate phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9027-40-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + pyruvate + phosphate
AMP + phosphoenolpyruvate + diphosphate
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + pyruvate + phosphate
AMP + phosphoenolpyruvate + diphosphate
show the reaction diagram
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-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025 - 0.049
ATP
0.118 - 0.138
phosphate
0.059 - 0.073
pyruvate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PPDK_FLABI
953
0
104002
Swiss-Prot
Chloroplast (Reliability: 1)
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
5 min 50% loss of activity
4
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dissociates in dimers when subjected to gel filtration at 4ºC or at 22°C but in the presence of EDTA
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
high levels of expression in Zea mays by using a double intron cassette and a chimeric cDNA made from Flaveria bidentis and Flaveria brownii with a maximum content of 1 mg/g fresh weight. In leaves of transgenic maize, PPDK molecules produced from the transgene are detected in cold-tolerant homotetramers or in heterotetramers of intermediate cold susceptibility formed with the internal PPDK
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Usami, S.; Ohta, S.; Komari, T.; Burnell, J.N.
Cold stability of pyruvate, orthophosphate dikinase of Flaveria brownii
Plant Mol. Biol.
27
969-980
1995
Zea mays, Flaveria brownii (Q39734), Flaveria brownii, Flaveria bidentis (Q39735), Flaveria bidentis
Manually annotated by BRENDA team
Ohta, S.; Usami, S.; Ueki, J.; Kumashiro, T.; Komari, T.; Burnell, J.N.
Identification of the amino acid residues responsible for cold tolerance in Flaveria brownii pyruvate,orthophosphate dikinase
FEBS Lett.
403
5-9
1997
Flaveria bidentis, Flaveria brownii, Zea mays
Manually annotated by BRENDA team
Ohta, S.; Ishida, Y.; Usami, S.
Expression of cold-tolerant pyruvate, orthophosphate dikinase cDNA, and heterotetramer formation in transgenic maize plants
Transgenic Res.
13
475-485
2004
Flaveria bidentis, Flaveria brownii, Zea mays
Manually annotated by BRENDA team