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Information on EC 2.7.8.7 - holo-[acyl-carrier-protein] synthase
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EC Tree
2.7.8.7 holo-[acyl-carrier-protein] synthaseIUBMB Comments
Requires Mg2+. All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active. The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain . The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty-acid synthase system (EC 2.3.1.85) and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier-proteins from prokaryotes . Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC 3.1.4.14, [acyl-carrier-protein] phosphodiesterase.
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Word Map
- 2.7.8.7
-
phosphopantetheinylation
- polyketide
-
4\'-phosphopantetheinyl
-
nonribosomal
- synthetases
-
peptidyl
-
siderophore
-
lipopeptide
-
nrpss
- apo-acp
- enterobactin
-
holo-forms
-
biosurfactant
-
fengycin
-
indigoidine
- biotechnology
- alpha-aminoadipate
- analysis
- drug development
- molecular biology
- medicine
- synthesis
-
iturins
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
+
=
+
+
an apo-[acyl-carrier protein]
=
+
Synonyms
pptase, phosphopantetheinyl transferase, surfactin synthetase, 4'-phosphopantetheinyl transferase, mtppt, type ii fatty acid synthase system, sfp-type pptase, schppt, holo-acyl carrier protein synthase, mtacps, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(FAS)ACP
4'-phosphopantetheinyl transferase
4-phosphopantetheinyl transferase
AcpS
acyl carrier protein holoprotein (holo-ACP) synthetase
-
-
-
-
acyl carrier protein synthase
acyl carrier protein synthetase
-
-
-
-
BcPPT
BPSS2266
coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine transferase
-
-
-
-
EntD
FKACPS
FKPPT1
FKPPT2
FKPPT3
FKPPT4
holo ACP synthase
holo-ACP synthase
holo-ACP synthetase
-
-
-
-
holo-acyl carrier protein synthase
holosynthase
-
-
-
-
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
UniProt
NpgA
P-pant transferase
PcpS
phosphopantetheinyl transferase
PP1183
PPT
PPT1
PPTase
PptB
PptT
PswP
PTase
Rv2794c
SchPPT
Sfp
sfp lpa-8
Sfp-type PPTase
surfactin phosphopantetheinyl transferase
type-II PPTase PptT
VabD
XabA
AcpS
-
acyl carrier protein synthases that recognize carrier protein from primary metabolism
holo-ACP synthase
-
-
-
-
phosphopantetheinyl transferase
close to the class two phosphopantetheinyl transferase Sfp from Bacillus subtilis
phosphopantetheinyl transferase
-
phosphopantetheinyl transferase
-
phosphopantetheinyl transferase
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CoA-[4'-phosphopantetheine] + an apo-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier protein]
CoA-[4'-phosphopantetheine] + an apo-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier protein]
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-
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CoA-[4'-phosphopantetheine] + an apo-[acyl-carrier protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier protein]
sequential binding mechanism: initial CoA- and Mg2+-binding followed by binding of acyl-carrier protein (ACP), nucleophilic attack of ACP-serine-hydroxylate on beta-phosphate of CoA followed by charge migration, Lys185-protonation, diphosphate-cleavage, and product dissociation, rate limiting step: release of 3',5'-ADP, key acid/base catalysts: residues E181 (CoA-binding) and K185 (Mg2+-binding)
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
substituted phospho group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
CoA-[4'-phosphopantetheine]:apo-[acyl-carrier protein] 4'-pantetheinephosphotransferase
Requires Mg2+. All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active. The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain [3]. The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty-acid synthase system (EC 2.3.1.85) and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier-proteins from prokaryotes [6]. Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC 3.1.4.14, [acyl-carrier-protein] phosphodiesterase.
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CAS REGISTRY NUMBER
COMMENTARY
37278-30-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-hydroxybutanoyl-CoA + apo-[acyl-carrier protein]
CoA + 3-hydroxybutanoyl-[acyl-carrier protein]
substrate Plasmodium falciparum apo-[acyl-carrier protein]
-
-
?
7-nitrobenz-2-oxa-1,3-diazol-4-yl-CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + 7-nitrobenz-2-oxa-1,3-diazol-4-yl-holo-[acyl-carrier protein]
acetoacetyl-CoA + apo-[acyl-carrier protein]
CoA + acetoacetyl-[acyl-carrier protein]
substrate Plasmodium falciparum apo-[acyl-carrier protein]
-
-
?
apo-[acyl-carrier protein] + acetyl-CoA
CoA + acetyl-[acyl-carrier protein]
pH 7, 37°C
reaction stop by 10% trichloroacetic acid, limited release of 3,5-ADP by interactions with guanidinium moieties of R74 and R86
-
?
benzoyl-CoA + apo-[acyl-carrier protein]
CoA + benzoyl-[acyl-carrier protein]
-
-
-
-
r
biotin-CoA + GDSLSWLLRSLN
GD-(biotinyl-4'-[N-{2-[2-(2-aminoethoxy)ethoxy]ethyl}-3-(2,5-dioxopyrrolidin-1-yl)propanamide]phosphopantetheinyl)SLSWLLRSLN + ?
butanoyl-CoA + apo-[acyl-carrier protein]
CoA + butanoyl-[acyl-carrier protein]
substrate Plasmodium falciparum apo-[acyl-carrier protein]
-
-
?
CoA + apo-[acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
CoA + apo-[alpha-aminoadipate semialdehyde dehydrogenase]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde dehydrogenase]
CoA + apo-[alpha-aminoadipate semialdehyde reductase Lys2]
adenosine 3',5'-bisphosphate + holo-[alpha-aminoadipate semialdehyde reductase Lys2]
-
-
-
-
r
CoA + apo-[fredericamycin H acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[fredericamycin acyl-carrier protein]
-
-
-
?
CoA + apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
? + holo-[acyl-carrier protein]
-
-
-
-
r
CoA + apo-[peptidyl carrier protein]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
CoA + apo-[peptidyl-carrier protein 1]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
-
-
?
CoA + apo-[peptidyl-carrier protein 2]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
-
-
?
CoA + apo-[peptidyl-carrier protein 3]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
-
-
?
CoA + apo-[peptidyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
-
-
-
?
CoA + apo-[Streptomyces sp. frenolicin-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. frenolicin-acyl-carrier protein]
-
-
-
-
r
CoA + apo-[Streptomyces sp. granaticin-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. granaticin-acyl-carrier protein]
-
-
-
-
r
CoA + apo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. oxytetracycline-acyl-carrier protein]
-
-
-
-
r
CoA + apo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
adenosine 3',5'-bisphosphate + holo-[Streptomyces sp. tetracenomycin-acyl-carrier protein(His6)]
-
-
-
-
r
CoA + apo-[tetracenomycin M acyl-carrier protein]
adenosine 3',5'-bisphosphate + holo-[tetracenomycin M acyl-carrier protein]