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Information on EC 2.7.8.41 - cardiolipin synthase (CMP-forming) and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q07560
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2.7.8.41 cardiolipin synthase (CMP-forming)IUBMB Comments
The eukaryotic enzyme is involved in the biosynthesis of the mitochondrial phospholipid cardiolipin. It requires divalent cations for activity.
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Word Map
- 2.7.8.41
- phospholipid
- phosphatidylglycerol
- phosphatidylethanolamine
- phosphatidylglycerophosphate
-
cl-deficient
-
non-fermentable
-
cl-rich
- medicine
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
hcls1, crls1, cls_cap, cls1p, cardiolipin synthase 1, crcls1, cls 1, sco1389, cardiolipin synthase-1, ydl142c, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:L-1-phosphatidyl-glycerol diacylglycerolphosphotransferase (CMP-forming)
The eukaryotic enzyme is involved in the biosynthesis of the mitochondrial phospholipid cardiolipin. It requires divalent cations for activity.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CDP-dioleoylglycerol + 1,2-dioleoylphosphatidylglycerol
1,3-bis(1,2-dioleoyl-sn-glycero-3-phospho)-sn-glycerol + CMP
-
-
-
?
a CDP-diacylglycerol + a phosphatidyl-glycerol
a cardiolipin + CMP
-
-
-
-
?
a CDP-diacylglycerol + a phosphatidylglycerol
a cardiolipin + CMP
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
a CDP-diacylglycerol + a phosphatidyl-glycerol
a cardiolipin + CMP
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
maximal activity is obtained in the presence of 20 mM Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Triton
maximal activity is obtained with a Triton X-100:phospholipid ratio of 1:1
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
the two Km values for CDP-diacylglycerol, each estimated by nonlinear regression analysis of 18 kinetic points, are 0.02 and 0.03 mol% in the presence of 1 mM and 10 mM Mg2+ respectively, pH 9.0, 37°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 10
pH 7.5: about 45% of maximal activity, pH 10.0: about 85% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35 - 65
35°C: about 45% of maximal activity, 65°C: about 40% of maximal activity
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
expression of CRD1 is essential for normal colony formation at elevated temperatures
physiological function
-
expression of Trypanosoma brucei CLS complements cardiolipin production in CRD1 knockout Saccharomyces cerevisiae and partly restores wild-type colony forming capability under stress conditions. Cardiolipin remodeling appears to be impaired in the transgenic construct. No complementation is observed by heterologous expression of Crd1 in conditional CLS knockout trypanosomes, despite proper mitochondrial targeting of the protein
malfunction
a yeast mutant with a deletion of YDL142c is defective in the formation of cardiolipin. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on nonfermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid of cardiolipin but accumulate the cardiolipin precursor phosphatidylglycerol when grown on nonfermentable carbon sources
malfunction
null mutant can grow on both fermentable and non-fermentable carbon sources at lower temperatures, it cannot form colonies at 37°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
28000
-
x * 28000, the functional enzyme is a large complex, the 28000 Da protein forms an oligomeric complex whose biogenesis and/or activity is influenced by the assembly of cytochrome oxidase, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 28000, the functional enzyme is a large complex, the 28000 Da protein forms an oligomeric complex whose biogenesis and/or activity is influenced by the assembly of cytochrome oxidase, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after CDP-diacylglycerol affinity chromatography the enzyme is very unstable to freeze-thaw cycles
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, stable for a few days, after CDP-diacylglycerol affinity chromatography
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CRD1 levels are 7- to 10-fold greater in stationary than in logarithmic growth phase. Expression is somewhat elevated during growth in glycerol/ethanol versus glucose media. CRD1 expression is controlled by factors affecting mitochondrial development, but not by the phospholipid precursors inositol and choline
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tamai, K.T.; Greenberg, M.L.
Biochemical characterization and regulation of cardiolipin synthase in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1046
214-222
1990
Saccharomyces cerevisiae (Q07560), Saccharomyces cerevisiae ATCC 204508 (Q07560)
Tuller, G.; Hrastnik, C.; Achleitner, G.; Schiefthaler, U.; Klein, F.; Daum, G.
YDL142c encodes cardiolipin synthase (Cls1p) and is non-essential for aerobic growth of Saccharomyces cerevisiae
FEBS Lett.
421
15-18
1998
Saccharomyces cerevisiae (Q07560), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q07560)
Zhao, M.; Schlame, M.; Rua, D.; Greenberg, M.L.
Cardiolipin synthase is associated with a large complex in yeast mitochondria
J. Biol. Chem.
273
2402-2408
1998
Saccharomyces cerevisiae
Schlame, M.; Zhao, M.; Rua, D.; Haldar, D.; Greenberg, M.L.
Kinetic analysis of cardiolipin synthase: a membrane enzyme with two glycerophospholipid substrates
Lipids
30
633-640
1995
Rattus norvegicus, Saccharomyces cerevisiae
Jiang, F.; Gu, Z.; Granger, J.M.; Greenberg, M.L.
Cardiolipin synthase expression is essential for growth at elevated temperature and is regulated by factors affecting mitochondrial development
Mol. Microbiol.
31
373-379
1999
Saccharomyces cerevisiae (Q07560), Saccharomyces cerevisiae ATCC 204508 (Q07560)
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