Information on EC 2.7.8.35 - UDP-N-acetylglucosamine-decaprenyl-phosphate N-acetylglucosaminephosphotransferase

for references in articles please use BRENDA:EC2.7.8.35
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.8.35
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RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylglucosamine-decaprenyl-phosphate N-acetylglucosaminephosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate = UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:trans,octacis-decaprenyl-phosphate N-acetylglucosaminephosphotransferase
Isolated from Mycobacterium tuberculosis and Mycobacterium smegmatis. This enzyme catalyses the synthesis of monotrans,octacis-decaprenyl-N-acetyl-alpha-D-glucosaminyl diphosphate (mycobacterial lipid I), an essential lipid intermediate for the biosynthesis of various bacterial cell envelope components. cf. EC 2.7.8.33, UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase.
CAS REGISTRY NUMBER
COMMENTARY hide
313355-28-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene wecA
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CPZEN-45
a caprazamycin derivative, CPZEN-45 shows excellent antitubercular activity against not only drug-sensitive strains but also some multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis strains
Rifampin
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the sensitivity of wild-type mc2155 strain against rifampin is enhanced in the presence of low concentrations of tunicamycin, a natural WecA inhibitor. An enzyme knockdown mutant strain also shows increased sensitivity to rifampin
tunicamycin
UT-01320
UT-03325
UT-03328
UT-03347
Vancomycin
weak inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00004
CPZEN-45
Mycobacterium tuberculosis
P9WMW5
pH and temperature not specified in the publication
0.004
Vancomycin
Mycobacterium tuberculosis
P9WMW5
pH and temperature not specified in the publication
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
preparation of membrane fraction
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in wecA-defective Escherichia coli MV501 cells
gene wecA, recombinant expression in Mycobacterium smegmatis strain 8a10
gene wecA, recombinant overexpression in Escherichia coli strain BL21
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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