Information on EC 2.7.8.34 - CDP-L-myo-inositol myo-inositolphosphotransferase

for references in articles please use BRENDA:EC2.7.8.34
Word Map on EC 2.7.8.34
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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.8.34
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RECOMMENDED NAME
GeneOntology No.
CDP-L-myo-inositol myo-inositolphosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate = CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
di-myo-inositol phosphate biosynthesis
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myo-inositol biosynthesis
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Inositol phosphate metabolism
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SYSTEMATIC NAME
IUBMB Comments
CDP-1L-myo-inositol:1L-myo-inositol 1-phosphate myo-inositolphosphotransferase
In many organisms this activity is catalysed by a bifunctional enzyme. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain of the bifunctional EC 2.7.7.74/EC 2.7.8.34 (CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase) uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-1L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors. The enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3-phosphate, a widespread organic solute in microorganisms adapted to hot environments.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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deleting the gene encoding the key enzyme in di-myo-inositol phosphate synthesis, CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase, abolishes di-myo-inositol phosphate synthesis. Mutants deficient in di-myo-inositol phosphate use aspartate to cope with heat stress
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
show the reaction diagram
CDP-D-myo-inositol + 1L-myo-inositol 1-phosphate
?
show the reaction diagram
CDP-glycerol + 1D-myo-inositol 3-phosphate
CMP + myo-L-inositol 1,1'-phosphoglycerol
show the reaction diagram
CDP-glycerol + 1L-myo-inositol 1-phosphate
CMP + 1L-(1X-glyceryl)-myo-inositol phosphate 3-phosphate
show the reaction diagram
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bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase. The cytidylyltransferase is absolutely specific for CTP and 1L-myo-inositol 1-phosphate. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
apo enzyme, sitting drop vapor diffusion method, using 1.2-1.4 M trisodium citrate dihydrate, 100 mM HEPES pH 6.8-7.2 and 10 mM MgCl2
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-Trap column chromatography and Superdex G75 gel filtration
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partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLyS cells
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expression in Escherichia coli