Information on EC 2.7.8.32 - 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose xylosylphosphotransferase

for references in articles please use BRENDA:EC2.7.8.32
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The expected taxonomic range for this enzyme is: Cryptococcus neoformans

EC NUMBER
COMMENTARY hide
2.7.8.32
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RECOMMENDED NAME
GeneOntology No.
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose xylosylphosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose = UMP + 3-O-(6-O-alpha-D-xylosylphospho-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose xylosylphosphotransferase
Mn2+ required for activity. The enzyme is specific for mannose as an acceptor but is flexible as to the structural context of the mannosyl disaccharide.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. grubii
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
show the reaction diagram
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
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?
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
show the reaction diagram
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
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?
UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
show the reaction diagram
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
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?
UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
show the reaction diagram
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
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?
additional information
?
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the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
15% of the activity compared to Mn2+ as metal ion cofactor
Mg2+
15% of the activity compared to Mn2+ as metal ion cofactor
Mn2+
preferred metal ion cofactor
additional information
no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the Xpt1 sequence contains a single preducted transmembrane domain
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
99527
x * 99527, calculated from sequence
99530
calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 99527, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity purification
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels