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Information on EC 2.7.8.29 - L-serine-phosphatidylethanolamine phosphatidyltransferase and Organism(s) Mus musculus and UniProt Accession Q99LH2

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IUBMB Comments
This mammalian enzyme catalyses an exchange reaction in which the polar head group of phosphatidylethanolamine is replaced by L-serine.
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This record set is specific for:
Mus musculus
UNIPROT: Q99LH2
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
ptdss1, pss-2, ptdss2, phosphatidylserine synthase 2, ps synthase 1, pssii, ps synthase 2, ptdser synthase 2, phosphatidylserine synthetase 2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphatidylserine synthase 1
-
PS synthase 1
-
phosphatidylserine synthase 2
-
PS synthase 2
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
L-1-phosphatidylethanolamine:L-serine phosphatidyltransferase
This mammalian enzyme catalyses an exchange reaction in which the polar head group of phosphatidylethanolamine is replaced by L-serine.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-1-phosphatidylcholine + L-serine
L-1-phosphatidylserine + choline
show the reaction diagram
isozyme PSS1
-
-
r
1,2-diacylphosphatidylethanolamine + L-serine
?
show the reaction diagram
the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen
-
-
?
L-1-phosphatidylethanolamine + L-serine
L-1-phosphatidylserine + ethanolamine
show the reaction diagram
phosphatidylethanolamine plasmalogen + L-serine
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-1-phosphatidylcholine + L-serine
L-1-phosphatidylserine + choline
show the reaction diagram
isozyme PSS1
-
-
r
L-1-phosphatidylethanolamine + L-serine
L-1-phosphatidylserine + ethanolamine
show the reaction diagram
isozyme PSS2
-
-
r
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high level of expression
Manually annotated by BRENDA team
-
high level of expression
Manually annotated by BRENDA team
-
high level of expression
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2
metabolism
phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PTSS1_MOUSE
473
9
55604
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anti-FLAG M2 affinity gel chromatography
purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293 cells
expression in McArdle cell and in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, EC 2.7.8.29
gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
docosahexaenoic acid positively modulates phosphatidylserine biosynthesis but does not affect mRNA levels of PSS2
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Arikketh, D.; Nelson, R.; Vance, J.E.
Defining the importance of phosphatidylserine synthase-1 (PSS1): unexpected viability of PSS1-deficient mice
J. Biol. Chem.
283
12888-12897
2008
Mus musculus
Manually annotated by BRENDA team
Guo, M.; Stockert, L.; Akbar, M.; Kim, H.Y.
Neuronal specific increase of phosphatidylserine by docosahexaenoic acid
J. Mol. Neurosci.
33
67-73
2007
Cricetulus griseus (O08888), Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Stone, S.J.; Vance, J.E.
Cloning and expression of murine liver phosphatidylserine synthase (PSS)-2: differential regulation of phospholipid metabolism by PSS1 and PSS2
Biochem. J.
342
57-64
1999
Mus musculus (Q9Z1X2)
Manually annotated by BRENDA team
Bergo, M.O.; Gavino, B.J.; Steenbergen, R.; Sturbois, B.; Parlow, A.F.; Sanan, D.A.; Skarnes, W.C.; Vance, J.E.; Young, S.G.
Defining the importance of phosphatidylserine synthase 2 in mice
J. Biol. Chem.
277
47701-47708
2002
Mus musculus
Manually annotated by BRENDA team
Kimura, A.K.; Kim, H.Y.
Phosphatidylserine synthase 2: high efficiency for synthesizing phosphatidylserine containing docosahexaenoic acid
J. Lipid Res.
54
214-222
2013
Mus musculus, Mus musculus (Q99LH2), Mus musculus (Q9Z1X2)
Manually annotated by BRENDA team