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Information on EC 2.7.8.29 - L-serine-phosphatidylethanolamine phosphatidyltransferase and Organism(s) Cricetulus griseus and UniProt Accession O08888
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2.7.8.29 L-serine-phosphatidylethanolamine phosphatidyltransferaseIUBMB Comments
This mammalian enzyme catalyses an exchange reaction in which the polar head group of phosphatidylethanolamine is replaced by L-serine.
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- phospholipid
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intellectual
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docosahexaenoic
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osteosclerosis
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craniofacial
- medicine
The taxonomic range for the selected organisms is: Cricetulus griseus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
ptdss1, pss-2, ptdss2, phosphatidylserine synthase 2, ps synthase 1, pssii, ps synthase 2, ptdser synthase 2, phosphatidylserine synthetase 2, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PATHWAY SOURCE
PATHWAYS
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-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
L-1-phosphatidylethanolamine:L-serine phosphatidyltransferase
This mammalian enzyme catalyses an exchange reaction in which the polar head group of phosphatidylethanolamine is replaced by L-serine.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-1-phosphatidylethanolamine + L-serine
L-1-phosphatidylserine + ethanolamine
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-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
phosphatidylserine
inhibition of purified enzyme and the crude enzyme in a membrane fraction
phosphatidylserine
The phosphatidylserine synthesis in a CHO cell mutant which lacks PSS I, EC 2.7.8.8, but has normal PSS II activity, is almost completely inhibited by the addition of phosphatidylserine to the culture medium, like that in the wild-type CHO-K1 cells. The phosphatidylserine synthesis in a PSS II-overproducing stable transformant is reduced by 35% upon addition of phosphatidylserine. Residue Arg-97 is critical for the exogenous phosphatidylserine-mediated inhibition
additional information
not inhibitory: phosphatidylethanolamine, phosphatidylcholine
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additional information
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not inhibitory: phosphatidylethanolamine, phosphatidylcholine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
phosphatidylserine biosynthesis in Chinese hamster ovary cells increases 2.5fold during UV-induced apoptosis. When cells are exposed to UV light to induce apoptosis, phosphatidylserine biosynthesis is stimulated 2fold in PSS II-expressing cells
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PSS2 of CHO-K1 cells is present only in mitochondria-associated membranes
PSS2 of CHO-K1 cells is present only in mitochondria-associated membranes
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
docosahexaenoic acid positively modulates phosphatidylserine biosynthesis. Over-expression of PSS2 alters neither the phosphatidylserine level nor the effect of docosahexaenoic acid on phosphatidylserine increase
physiological function
induced apoptosis with staurosporine in four Chinese hamster ovary cell lines that are deficient in PSS1, EC 2.7.8.8, and/or PSS2. In all cell lines, regardless of their content of PSS1 and/or PSS2, apoptosis occurrs to approximately the same extent, and within approximately the same time frame, as in parental CHO-K1 cells. Cells that are deficient in either PSS1 or PSS2, as well as cells that are deficient in both PSS1 and PSS2, externalize normal amounts of phosphatidylserine
physiological function
introduction of the pssB cDNA into CHO-K1 cells results in striking increases in both the serine and ethanolamine base exchange activities. The pssB cDNA is incapable of increasing the choline base exchange activity. The expression of the pssB gene in Sf9 insect cells also results in striking increases in both serine and ethanolamine base exchange activities. The pssB cDNA transforms a phosphatidylserine-auxotrophic mutant of CHO-K1 cells lacking PSS I, EC 2.7.8.8, to phosphatidylserine prototrophy. The phosphatidylserine content of the resultant transformant grown without exogenous phosphatidylserine for 2 days is 4-fold that of the mutant and similar to that of CHO-K1 cells, indicating that the pssB cDNA complements the phosphatidylserine biosynthetic defect of the PSS I-lacking mutant
physiological function
mutant defective in PSS II show 5% of the activity in the homogenate CHO-K1 cells and 10% of the activity in the homogenate of cells lacking PSS I, EC 2.7.8.8. The PSS II mutant grows well in medium supplemented with phosphatidylserine. However, in the medium supplemented with phosphatidylethanolamine, the PSS II-mutant is incapable of growth. In the medium with exogenous phosphatidylethanolamine, the PSS II-mutant is defective in phosphatidylserine biosynthesis
physiological function
phosphatidylserine biosynthesis in Chinese hamster ovary cells increases 2.5fold during UV-induced apoptosis and is not reversed by caspase inhibitor, Z-VAD-FMK, i.e. benzyloxycarbonyl-Val-Ala-DL-Asp-fluoromethylketone. Stimulation of synthesis is less specific for phosphatidylserine as similar levels of stimulation are observed for sphingomyelin biosynthesis. PSS I- , EC 2.7.8.8, or PSS II-expressing cells have higher basal levels of phosphatidylserine biosynthesis compared with vector control cells. When cells are exposed to UV light to induce apoptosis, phosphatidylserine biosynthesis is further stimulated 1.5- and 2fold in PSS I- and PSS II-expressing cells respectively. Cells overexpressing PSS I and II are actually resistant to UV-induced apoptosis
physiological function
PSS II activity is inhibited by exogenous phosphatidylserine and overproduction of PSS II leads to the loss of normal control of PSS II activity by exogenous phosphatidylserine. PSS II-overproducing cells cultivated without exogenous phosphatidylserine exhibit a normal phosphatidylserine biosynthetic rate similar to that in CHO-K1 cells. Stable transformation of R97K mutant PSS II, leads to a 4fold higher phosphatidylserine biosynthetic rate
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PTSS2_CRIGR
474
7
55005
Swiss-Prot
Mitochondrion (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
R97K
in contrast to the PSS II wild-type transformant, the R97K transformant exhibits 4fold higher phosphatidylserine biosynthetic activity than that in CHO-K1 cells. The phosphatidylserine biosynthesis in the R97K transformant is not inhibited at all but elevated by the addition of phosphatidylserine
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
docosahexaenoic acid positively modulates phosphatidylserine biosynthesis but does not affect mRNA levels of PSS2
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yu, A.; McMaster, C.R.; Byers, D.M.; Ridgway, N.D.; Cook, H.W.
Resistance to UV-induced apoptosis in Chinese-hamster ovary cells overexpressing phosphatidylserine synthases
Biochem. J.
381
609-618
2004
Cricetulus griseus (O08888)
Grandmaison, P.A.; Nanowski, T.S.; Vance, J.E.
Externalization of phosphatidylserine during apoptosis does not specifically require either isoform of phosphatidylserine synthase
Biochim. Biophys. Acta
1636
1-11
2004
Cricetulus griseus (O08888)
Guo, M.; Stockert, L.; Akbar, M.; Kim, H.Y.
Neuronal specific increase of phosphatidylserine by docosahexaenoic acid
J. Mol. Neurosci.
33
67-73
2007
Cricetulus griseus (O08888), Homo sapiens, Mus musculus
Kuge, O.; Saito, K.; Nishijima, M.
Cloning of a Chinese hamster ovary (CHO) cDNA encoding phosphatidylserine synthase (PSS) II, overexpression of which suppresses the phosphatidylserine biosynthetic defect of a PSS I-lacking mutant of CHO-K1 cells
J. Biol. Chem.
272
19133-19139
1997
Cricetulus griseus (O08888)
Saito, K.; Nishijima, M.; Kuge, O.
Genetic evidence that phosphatidylserine synthase II catalyzes the conversion of phosphatidylethanolamine to phosphatidylserine in Chinese hamster ovary cells
J. Biol. Chem.
273
17199-17205
1998
Cricetulus griseus (O08888)
Kuge, O.; Saito, K.; Nishijima, M.
Control of phosphatidylserine synthase II activity in Chinese hamster ovary cells
J. Biol. Chem.
274
23844-23849
1999
Cricetulus griseus (O08888)
Stone, S.J.; Vance, J.E.
Phosphatidylserine synthase-1 and -2 are localized to mitochondria-associated membranes
J. Biol. Chem.
275
34534-34540
2000
Rattus norvegicus, Cricetulus griseus (O08888)
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