Information on EC 2.7.8.26 - adenosylcobinamide-GDP ribazoletransferase

for references in articles please use BRENDA:EC2.7.8.26
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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
2.7.8.26
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RECOMMENDED NAME
GeneOntology No.
adenosylcobinamide-GDP ribazoletransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleosidyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenosylcobalamin biosynthesis from cobyrinate a,c-diamide I
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adenosylcobalamin biosynthesis from cobyrinate a,c-diamide II
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adenosylcobalamin salvage from cobinamide I
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adenosylcobalamin salvage from cobinamide II
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vitamin B12 metabolism
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Porphyrin and chlorophyll metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
adenosylcobinamide-GDP:alpha-ribazole ribazoletransferase
In Salmonella typhimurium LT2, under anaerobic conditions, CobU (EC 2.7.7.62 and EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) and CobS (EC 2.7.8.26) catalyse reactions in the nucleotide loop assembly pathway, which convert adenosylcobinamide (AdoCbi) into adenosylcobalamin (AdoCbl). CobT and CobC are involved in 5,6-dimethylbenzimidazole activation whereby 5,6-dimethylbenzimidazole is converted to its riboside, alpha-ribazole. The second branch of the nucleotide loop assembly pathway is the cobinamide activation branch where AdoCbi or adenosylcobinamide-phosphate is converted to the activated intermediate AdoCbi-GDP by the bifunctional enzyme Cob U. CobS catalyses the final step in adenosylcobalamin biosynthesis, which is the condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin.
CAS REGISTRY NUMBER
COMMENTARY hide
137672-85-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
KOD1
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Manually annotated by BRENDA team
KOD1
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Manually annotated by BRENDA team
serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
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Manually annotated by BRENDA team
; strains JE212 and JE2197
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosylcobinamide-GDP + alpha-ribazole
adenosylcobalamin + GMP
show the reaction diagram
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
show the reaction diagram
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
GMP + adenosylcobalamin 5'-phosphate
show the reaction diagram
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
GMP + adenosylcobalamin + phosphate + H+
show the reaction diagram
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?
alpha-ribazole + adenosylcobinamide-GDP
GMP + adenosylcobalamin
show the reaction diagram
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
GMP + adenosylcobalamin 5'-phosphate
show the reaction diagram
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
GMP + ?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosylcobinamide-GDP + alpha-ribazole
adenosylcobalamin + GMP
show the reaction diagram
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de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
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?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
show the reaction diagram
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
GMP + adenosylcobalamin 5'-phosphate
show the reaction diagram
P29936
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?
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
GMP + adenosylcobalamin + phosphate + H+
show the reaction diagram
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?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
GMP + ?
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0009
adenosylcobinamide-GDP
30C, pH 9.0
0.0078
alpha-ribazole
30C, pH 9.0
0.0027
alpha-ribazole 5'-phosphate
30C, pH 9.0
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.67
alpha-ribazole
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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associated with inner membrane, integral membrane protein
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25285
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x * 25285, deduced from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; nickel affinity chromatography, recombinant CobS
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solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+))
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Superose 12 HR, Phenyl-Superose, partially purified
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain C41(DE3)
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expression in Escherichia coli
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expression of His-tagged CobS in Escherichia coli; His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3)
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expression of truncated CobV missing the first 20 codons in Escherichia coli
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