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Enzyme Nomenclature
Information on EC 2.7.8.26 - adenosylcobinamide-GDP ribazoletransferase
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The expected taxonomic range for this enzyme is: Archaea, Bacteria
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EC NUMBER 
COMMENTARY 
2.7.8.26
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RECOMMENDED NAME
GeneOntology No.
adenosylcobinamide-GDP ribazoletransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nucleosidyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
5-methoxy-6-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP
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SYSTEMATIC NAME
IUBMB Comments
adenosylcobinamide-GDP:alpha-ribazole ribazoletransferase
In Salmonella typhimurium LT2, under anaerobic conditions, CobU (EC 2.7.7.62 and EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) and CobS (EC 2.7.8.26) catalyse reactions in the nucleotide loop assembly pathway, which convert adenosylcobinamide (AdoCbi) into adenosylcobalamin (AdoCbl). CobT and CobC are involved in 5,6-dimethylbenzimidazole activation whereby 5,6-dimethylbenzimidazole is converted to its riboside, alpha-ribazole. The second branch of the nucleotide loop assembly pathway is the cobinamide activation branch where AdoCbi or adenosylcobinamide-phosphate is converted to the activated intermediate AdoCbi-GDP by the bifunctional enzyme Cob U. CobS catalyses the final step in adenosylcobalamin biosynthesis, which is the condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin.
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CAS REGISTRY NUMBER
COMMENTARY
137672-85-6
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
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serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
GMP + adenosylcobalamin + phosphate + H+
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-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
adenosylcobalamin + GMP
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-
-
?
adenosylcobinamide-GDP + alpha-ribazole
adenosylcobalamin + GMP
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de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
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?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
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-
-
?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
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-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
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-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
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-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
GMP + adenosylcobalamin 5'-phosphate
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?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
GMP + adenosylcobalamin 5'-phosphate
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alpha-ribazole-5’-phosphate is a more preferred substrate compared to alpha-ribazole
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-
?
alpha-ribazole + adenosylcobinamide-GDP
GMP + adenosylcobalamin
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1 h, 37°C, pH 9, in dim light, equimolar substrate concentration
reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC
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?
alpha-ribazole + adenosylcobinamide-GDP
GMP + adenosylcobalamin
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1 h, 37°C, pH 9, in dim light, equimolar substrate concentration
reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC
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?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
GMP + adenosylcobalamin 5'-phosphate
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1 h, 37°C, pH 9, in dim light, equimolar substrate concentration
reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC
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?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
GMP + adenosylcobalamin 5'-phosphate
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1 h, 37°C, pH 9, in dim light, equimolar substrate concentration
reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC
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?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
GMP + ?
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second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
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?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
GMP + ?
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second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
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?
additional information
?
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about 100fold more efficient with alpha-ribazole-5’-phosphate as substrate than using alpha-ribazole, in vivo
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additional information
?
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about 100fold more efficient with alpha-ribazole-5’-phosphate as substrate than using alpha-ribazole, in vivo
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
adenosylcobinamide-GDP + alpha-ribazole
adenosylcobalamin + GMP
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de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
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?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
GMP + adenosylcobalamin 5'-phosphate
P29936
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?
adenosylcobinamide-GDP + alpha-ribazole-5'-phosphate + H2O
GMP + adenosylcobalamin + phosphate + H+
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-
?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
P29936
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?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
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-
?
adenosylcobinamide-GDP + alpha-ribazole
GMP + adenosylcobalamin
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-
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
GMP + ?
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second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
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-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
GMP + ?
-
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
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-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0009
adenosylcobinamide-GDP
30°C, pH 9.0
0.0027
alpha-ribazole 5'-phosphate
30°C, pH 9.0
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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associated with inner membrane, integral membrane protein
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; nickel affinity chromatography, recombinant CobS
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solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+))
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Superose 12 HR, Phenyl-Superose, partially purified
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of His-tagged CobS in Escherichia coli; His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3)
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expression of truncated CobV missing the first 20 codons in Escherichia coli
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.8.26)
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