In Salmonella typhimurium LT2, under anaerobic conditions, CobU (EC 2.7.7.62 and EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) and CobS (EC 2.7.8.26) catalyse reactions in the nucleotide loop assembly pathway, which convert adenosylcobinamide (AdoCbi) into adenosylcobalamin (AdoCbl). CobT and CobC are involved in 5,6-dimethylbenzimidazole activation whereby 5,6-dimethylbenzimidazole is converted to its riboside, alpha-ribazole. The second branch of the nucleotide loop assembly pathway is the cobinamide activation branch where AdoCbi or adenosylcobinamide-phosphate is converted to the activated intermediate AdoCbi-GDP by the bifunctional enzyme Cob U. CobS catalyses the final step in adenosylcobalamin biosynthesis, which is the condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin.
In Salmonella typhimurium LT2, under anaerobic conditions, CobU (EC 2.7.7.62 and EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) and CobS (EC 2.7.8.26) catalyse reactions in the nucleotide loop assembly pathway, which convert adenosylcobinamide (AdoCbi) into adenosylcobalamin (AdoCbl). CobT and CobC are involved in 5,6-dimethylbenzimidazole activation whereby 5,6-dimethylbenzimidazole is converted to its riboside, alpha-ribazole. The second branch of the nucleotide loop assembly pathway is the cobinamide activation branch where AdoCbi or adenosylcobinamide-phosphate is converted to the activated intermediate AdoCbi-GDP by the bifunctional enzyme Cob U. CobS catalyses the final step in adenosylcobalamin biosynthesis, which is the condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin.
de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5â-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5â-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5â-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5â-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+))
Cameron, B.; Blanche, F.; Rouyez, M.C.; Bisch, D.; Famechon, A.; Couder, M.; Cauchois, L.; Thibaut, D.; Debussche, L.; Crouzet, J.
Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5-phosphate) synthase
Reassessment of the late steps of coenzyme B12 synthesis in Salmonella enterica: evidence that dephosphorylation of adenosylcobalamin-5-phosphate by the CobC phosphatase is the last step of the pathway
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2.7.8.26 adenosylcobinamide-GDP ribazoletransferase
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