Information on EC 2.7.8.21 - membrane-oligosaccharide glycerophosphotransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

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EC NUMBER
COMMENTARY hide
2.7.8.21
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RECOMMENDED NAME
GeneOntology No.
membrane-oligosaccharide glycerophosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
transfer of a glycerophospho group from one membrane-derived oligosaccharide to another
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
membrane-derived-oligosaccharide-6-(glycerophospho)-D-glucose:membrane-derived-oligosaccharide-D-glucose glycerophosphotransferase
beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors. In the presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed.
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CAS REGISTRY NUMBER
COMMENTARY hide
80146-86-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-gentiobiose
?
show the reaction diagram
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-
-
-
?
membrane-derived oligosaccharides
?
show the reaction diagram
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-
-
-
?
membrane-derived oligosaccharides
additional information
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
membrane-derived oligosaccharides
?
show the reaction diagram
-
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
less effective than Mn2+
Co2+
-
less effective than Mn2+
Mg2+
-
less effective than Mn2+
Mn2+
-
absolute requirement for a divalent cation, Mn2+ most active
additional information
-
absolute requirement for a divalent cation
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cellobiose
-
5 mM, competitive to membrane-derived oligosaccharides, 77% of the activity without addition
D-glucose
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5 mM, competitive to membrane-derived oligosaccharides, 78% of the activity without addition
D-maltose
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5 mM, competitive to membrane-derived oligosaccharides, 82% of the activity without addition
gentiobiose
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5 mM, competitive to membrane-derived oligosaccharides, 15% of the activity without addition
sophorose
-
5 mM, competitive to membrane-derived oligosaccharides, 56% of the activity without addition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
gentiobiose
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pH 7.8, 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.8
-
active
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
gel filtration
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.8.21)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)