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Information on EC 2.7.8.21 - membrane-oligosaccharide glycerophosphotransferase for references in articles please use BRENDA:EC2.7.8.21
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EC Tree
IUBMB Comments beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors. In the presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed.
The expected taxonomic range for this enzyme is: Escherichia coli
Reaction Schemes
transfer of a glycerophospho group from one membrane-derived oligosaccharide to another
Synonyms
periplasmic phosphoglycerotransferase, Phosphoglycerol cyclase,
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periplasmic phosphoglycerotransferase
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Phosphoglycerol cyclase
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transfer of a glycerophospho group from one membrane-derived oligosaccharide to another
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substituted phospho group transfer
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membrane-derived-oligosaccharide-6-(glycerophospho)-D-glucose:membrane-derived-oligosaccharide-D-glucose glycerophosphotransferase
beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors. In the presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed.
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D-gentiobiose
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membrane-derived oligosaccharides
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membrane-derived oligosaccharides
additional information
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membrane-derived oligosaccharides
additional information
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transfer of a glycerophospho group from one membrane-derived oligosaccharide to another. beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors
in presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed
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additional information
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no activity detected with phosphatidylglycerol as donor, or suspensions of phospholipid mixed with Triton X-100 or in mixed vesicles with phosphatidylcholine
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membrane-derived oligosaccharides
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Ca2+
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less effective than Mn2+
Co2+
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less effective than Mn2+
Mg2+
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less effective than Mn2+
Mn2+
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absolute requirement for a divalent cation, Mn2+ most active
additional information
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absolute requirement for a divalent cation
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cellobiose
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5 mM, competitive to membrane-derived oligosaccharides, 77% of the activity without addition
D-glucose
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5 mM, competitive to membrane-derived oligosaccharides, 78% of the activity without addition
D-maltose
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5 mM, competitive to membrane-derived oligosaccharides, 82% of the activity without addition
gentiobiose
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5 mM, competitive to membrane-derived oligosaccharides, 15% of the activity without addition
sophorose
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5 mM, competitive to membrane-derived oligosaccharides, 56% of the activity without addition
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1
gentiobiose
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pH 7.8, 37°C
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K-12
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A0A0E1CPX0_KLEPN
763
4
85232
TrEMBL
Q3Y1E7_ENTFC
619
5
71055
TrEMBL
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Wang, P.; Ingram-Smith, C.; Hadley, J.A.; Miller, K.J.
Cloning, sequencing, and characterization of the cgmB gene of Sinorhizobium meliloti involved in cyclic beta-glucan biosynthesis
J. Bacteriol.
181
4576-4583
1999
Escherichia coli
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Goldberg, D.E.; Rumley, M.K.; Kennedy, E.P.
Biosynthesis of membrane-derived oligosaccharides: a periplasmic phosphoglyceroltransferase
Proc. Natl. Acad. Sci. USA
78
5513-5517
1981
Escherichia coli
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