Information on EC 2.7.8.21 - membrane-oligosaccharide glycerophosphotransferase

for references in articles please use BRENDA:EC2.7.8.21
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.8.21
-
RECOMMENDED NAME
GeneOntology No.
membrane-oligosaccharide glycerophosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
transfer of a glycerophospho group from one membrane-derived oligosaccharide to another
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
membrane-derived-oligosaccharide-6-(glycerophospho)-D-glucose:membrane-derived-oligosaccharide-D-glucose glycerophosphotransferase
beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors. In the presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed.
CAS REGISTRY NUMBER
COMMENTARY hide
80146-86-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-gentiobiose
?
show the reaction diagram
-
-
-
-
?
membrane-derived oligosaccharides
?
show the reaction diagram
-
-
-
-
?
membrane-derived oligosaccharides
additional information
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
membrane-derived oligosaccharides
?
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
less effective than Mn2+
Co2+
-
less effective than Mn2+
Mg2+
-
less effective than Mn2+
Mn2+
-
absolute requirement for a divalent cation, Mn2+ most active
additional information
-
absolute requirement for a divalent cation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cellobiose
-
5 mM, competitive to membrane-derived oligosaccharides, 77% of the activity without addition
D-glucose
-
5 mM, competitive to membrane-derived oligosaccharides, 78% of the activity without addition
D-maltose
-
5 mM, competitive to membrane-derived oligosaccharides, 82% of the activity without addition
gentiobiose
-
5 mM, competitive to membrane-derived oligosaccharides, 15% of the activity without addition
sophorose
-
5 mM, competitive to membrane-derived oligosaccharides, 56% of the activity without addition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
gentiobiose
-
pH 7.8, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.8
-
active
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE