Information on EC 2.7.8.20 - phosphatidylglycerol-membrane-oligosaccharide glycerophosphotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.8.20
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RECOMMENDED NAME
GeneOntology No.
phosphatidylglycerol-membrane-oligosaccharide glycerophosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphatidylglycerol + membrane-derived-oligosaccharide D-glucose = 1,2-diacyl-sn-glycerol + membrane-derived-oligosaccharide 6-(glycerophospho)-D-glucose
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
phosphatidylglycerol:membrane-derived-oligosaccharide-D-glucose glycerophosphotransferase
1,2-beta- and 1,6-beta-linked glucose residues in membrane polysaccharides and in synthetic glucosides can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
80146-86-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Rhizobium meliloti
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphatidylglycerol + (R)-alpha[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxyl]benzeneacetonitrile
sn-1,2-diacylglycerol + (R)-alpha[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxyl]benzeneacetonitrile-6-phosphoglycerol
show the reaction diagram
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i.e. amygdalin
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?
phosphatidylglycerol + beta-gentiobiose
sn-1,2-diacylglycerol + beta-gentiobiose-6-phosphoglycerol
show the reaction diagram
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose
sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol
show the reaction diagram
phosphatidylglycerol + o-nitrophenyl-beta-D-glucoside
sn-1,2-diacylglycerol + o-nitrophenyl-beta-D-glucoside-6-phosphoglycerol
show the reaction diagram
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?
phosphatidylglycerol + octyl-beta-D-glucoside
sn-1,2-diacylglycerol + octyl-beta-D-glucoside-6-phosphoglycerol
show the reaction diagram
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?
phosphatidylglycerol + p-hydroxyphenyl-beta-D-glucoside
sn-1,2-diacylglycerol + p-hydroxyphenyl-beta-D-glucoside-6-phosphoglycerol
show the reaction diagram
phosphatidylglycerol + p-nitrophenyl-beta-D-glucoside
sn-1,2-diacylglycerol + p-nitrophenyl-beta-D-glucoside-6-phosphoglycerol
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose
sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol
show the reaction diagram
additional information
?
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the membrane-bound phosphoglycerol transferase I transfers residues from membrane phosphatidylglycerol to nascent osmoregulated periplasmic glucan molecules. Phosphoglycerol transferase II is a soluble periplasmic form of phosphoglycerol transferase I, both activities, phosphoglycerol transferase I and II, are necessary to complete osmoregulated periplasmic glucan substitution
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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divalent cation required, Ca2+ is less effective than Mn2+
Mg2+
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divalent cation required, less effective than Mn2+. At the optimal concentration, 0.5 mM, the activity is 67% of that observed with the same concentration of MnCl2
Mn2+
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divalent cation required, Mn2+ is the most effective
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arbutin
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at 5 mM, competitive to membrane-derived oligosaccharides
beta-gentiobiose
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at 1-5 mM, competitive to membrane-derived oligosaccharides
EDTA
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at 0.1 mM, for 1 h at 0C, activity is reduced to 40% of the control value. The activity is fully restored by addition of 0.5 mM MnCl2
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 3
arbutin
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pH 4.5, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 9.7
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50% of optimal activity at pH 7.8 and pH 9.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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the active site is localized on the periplasmic face of the inner membrane
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Manually annotated by BRENDA team
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membrane-bound
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in an enzyme defective Escherichia coli mutant strain
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expression in an enzyme defective Sinorhizobium meliloti mutant strain
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Show AA Sequence (891 entries)
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