Information on EC 2.7.8.19 - UDP-glucose-glycoprotein glucose phosphotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.8.19
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RECOMMENDED NAME
GeneOntology No.
UDP-glucose-glycoprotein glucose phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + glycoprotein D-mannose = UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:glycoprotein-D-mannose glucosephosphotransferase
Penultimate mannose residues on oligo-mannose type glycoproteins can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
84861-40-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
strains NY13 and YDB135
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-azidouridine 5'-diphosphoglucose + glycoprotein D-mannose
?
show the reaction diagram
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?
UDP-glucose + glycoprotein D-mannose
UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
show the reaction diagram
UDP-glucose + parafusin
?
show the reaction diagram
UDP-glucose + phosphoglucomutase
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + parafusin
?
show the reaction diagram
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reversible phosphoglycosylation of parafusin could play an important role in signal transduction during secretion in Paramecium tetraurelia, a protein evidently associated with membrane fusion during exocytosis
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?
UDP-glucose + phosphoglucomutase
?
show the reaction diagram
additional information
?
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may be a controlling enzyme for targeting of certain newly synthesized proteins to the cell surface
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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added in the activity assay at a final concentration of 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
uteroferrin
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additional information
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cathepsin D and beta-N-acetylhexosaminidase have no significant effects on phosphoglucose incorporation
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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assay at, 50 mM Bis-Tris buffer
6.3
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10 mM sodium cacodylate buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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normal and I-cell
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
active fractions are frozen in a dry ice/methanol bath immediately after preparation and stored at -80°C
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