Information on EC 2.7.8.14 - CDP-ribitol ribitolphosphotransferase

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The expected taxonomic range for this enzyme is: Staphylococcus aureus

EC NUMBER
COMMENTARY hide
2.7.8.14
-
RECOMMENDED NAME
GeneOntology No.
CDP-ribitol ribitolphosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
n CDP-ribitol + 4-O-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n CMP + 4-O-(D-ribitylphospho)n-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus)
-
-
SYSTEMATIC NAME
IUBMB Comments
CDP-ribitol:4-O-di[(2R)-1-glycerophospho]-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol ribitolphosphotransferase
Involved in the biosynthesis of poly ribitol phosphate teichoic acids in the cell wall of the bacterium Staphylococcus aureus. This enzyme adds around 40 ribitol units to the linker molecule.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-63-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDPribitol + (ribitol phosphate)n
CMP + (ribitol phosphate)n+1
show the reaction diagram
CDPribitol + lipoteichoic acid
lipoteichoic acid-(P-ribitol)n + CMP
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDPribitol + (ribitol phosphate)n
CMP + (ribitol phosphate)n+1
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
slight stimulation
Mg2+
-
Mn2+ or Mg2+ required: 10-30 mM
Mn2+
-
Mn2+ or Mg2+ required, optimal concentration: 10-30 mM
additional information
-
no effect: Ni2+, Zn2+, Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-hydroxymercuribenzoate
-
inhibition at 0.4 mM, can be reversed by 2-mercaptoethanol
CDPglycerol
-
weak
Gentian violet
-
-
novobiocin
-
-
Ristocetin
-
-
additional information
-
no inhibition: penicillin G, staphcillin, alpha-aminobenzyl-penicillin, bacitracin
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cardiolipin
-
stimulates 6fold at 0.36 mM
lipoteichoic acid
-
required as carrier
-
phosphatidylcholine
-
stimulates 2.7fold 0.36 mM
phosphatidylethanolamine
-
stimulates 5fold 0.36 mM
phosphatidylglycerol
-
stimulates 4fold 0.36 mM
Phospholipid
-
required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
CDPribitol
-
in presence of Mn2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
24 h, very stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE