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Information on EC 2.7.8.11 - CDP-diacylglycerol-inositol 3-phosphatidyltransferase and Organism(s) Homo sapiens and UniProt Accession O14735

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Homo sapiens
UNIPROT: O14735 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
phosphatidylinositol synthase, pi synthase, ptdins synthase, cdipt, zmpis, cdp-diglyceride:inositol transferase, cdp-dg:inositol transferase, pkpis, cdp-diacylglycerol-inositol 3-phosphatidyltransferase, bnptdins s1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PtdIns synthase
-
CDP-DG:inositol transferase
-
-
-
-
CDP-diacylglycerol-inositol phosphatidyltransferase
-
-
-
-
CDP-diacylglycerol:myo-inositol-3-phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol transferase
-
-
-
-
CDP-diglyceride:inositol transferase
-
-
-
-
CDPdiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:myo-inositol 3-phosphatidyltransferase
-
-
-
-
cytidine diphosphodiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol transferase
-
-
-
-
phosphatidylinositol synthase
PI synthase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-01-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
r
2-deoxy-CDP-diacylglycerol + myo-inositol
CMP + ?
show the reaction diagram
CDP-diacylglycerol + myo-inositol
CMP + 1-phosphatidyl-1D-myo-inositol
show the reaction diagram
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
additional information
?
-
-
UDP-diacylglycerol, ADP-diacylglycerol, and GDP-diacylglycerol are inactive as substrates
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + 1-phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
-
-
CDP
-
competitive inhibition
CDP-diacylglycerol
-
above 0.5 mM
diphosphate
-
-
hexachlorocyclohexane
Inostamycin
-
-
phosphate
-
-
Zn2+
-
-
ZnCl2
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
2-deoxy-CDP-diacylglycerol
-
-
0.036
CDP-diacylglycerol
-
-
0.28
myo-inositol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.1
ADP
-
pH 9.0, 37°C
1.4
ATP
-
pH 9.0, 37°C
0.6
CaCl2
-
pH 9.0, 37°C
4
CDP
-
pH 9.0, 37°C
1.8
CTP
-
pH 9.0, 37°C
0.2
diphosphate
1.6
GTP
-
pH 9.0, 37°C
0.6 - 0.8
phosphate
1.4
TTP
-
pH 9.0, 37°C
3.8
UDP
-
pH 9.0, 37°C
1.4
UTP
-
pH 9.0, 37°C
2
ZnCl2
-
pH 9.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
in glycylglycine/NaOH or glycine/NaOH buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
human
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
carcinoma cell
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
membrane-bound
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
in eukarya, phosphatidylinositol (PI) is only biosynthesized from CDP-diacylglycerol (CDP-DAG) and inositol
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDIPT_HUMAN
213
4
23539
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
-
SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Homo sapiens cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 7 pmol/h/mg and 2 pmol/h/mg, respectively
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
half-life without substrates 20 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, frozen and stored or frozen in liquid nitrogen and subsequently stored, over 50% activity is lost after 24 h
-
-60°C, frozen and stored or frozen in liquid nitrogen and subsequently stored, over 50% activity is lost after 24 h
-
-80°C, regularly frozen in liqid N2, no detectable loss of activity is experienced during storage at -80°C for up to 2 months
-
4°C, stored on ice, 10% of the activity is lost during 24 h
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
yeast cDNA S45885 used to search, pis1 cDNA cloned and expressed in COS-7 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lykidis, A.; Jackson, P.; Rock, C.O.; Jackowski, S.
The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content
J. Biol. Chem.
272
33402-33409
1997
Homo sapiens (O14735), Homo sapiens
Manually annotated by BRENDA team
Imoto, M.; Taniguchi, Y.; Umezawa, K.
Inhibition of CDP-DG: inositol transferase by inostamycin
J. Biochem.
112
299-302
1992
Homo sapiens
Manually annotated by BRENDA team
Antonsson, B.E.
Purification and characterization of phosphatidylinositol synthase from human placenta
Biochem. J.
297
517-522
1994
Homo sapiens
-
Manually annotated by BRENDA team
Antonsson, B.
Phosphatidylinositol synthase from mammalian tissues
Biochim. Biophys. Acta
1348
179-186
1997
Bos taurus, Saccharomyces cerevisiae, Cavia porcellus, Gallus gallus, Oryctolagus cuniculus, Homo sapiens, Meleagris gallopavo, Rattus norvegicus
Manually annotated by BRENDA team
Morii, H.; Ogawa, M.; Fukuda, K.; Taniguchi, H.
Ubiquitous distribution of phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase in bacteria and archaea, which contain inositol phospholipid
Biochem. Biophys. Res. Commun.
443
86-90
2013
Homo sapiens, Saccharomyces cerevisiae (P70500), Saccharomyces cerevisiae
Manually annotated by BRENDA team