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Information on EC 2.7.8.1 - ethanolaminephosphotransferase and Organism(s) Homo sapiens and UniProt Accession Q9Y6K0

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Homo sapiens
UNIPROT: Q9Y6K0 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ept, ethanolaminephosphotransferase, ethanolamine phosphotransferase, hept1, cpt1p, ept1p, diacylglycerol ethanolaminephosphotransferase, selenoprotein 1, choline-ethanolamine phosphotransferase, cdp-ethanolamine:diacylglycerol ethanolaminephosphotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CEPT1
cf. EC 2.7.8.2
choline-ethanolamine phosphotransferase
-
AAPT
-
-
-
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aminoalcoholphosphotransferase
-
-
-
-
CDP-ethanolamine diglyceride phosphotransferase
-
-
-
-
CDP-ethanolamine:diacylglycerol ethanolaminephosphotransferase
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CEPT1
-
-
-
-
diacylglycerol ethanolaminephosphotransferase
-
-
-
-
ethanolamine phosphotransferase
-
-
-
-
ethanolaminephosphotransferase 1
-
ethanolaminephosphotransferase, diacylglycerol
-
-
-
-
hEPT1
phosphorylethanolamine-glyceride transferase
-
-
-
-
selenoprotein 1
-
additional information
cf. EC 2.7.8.2
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
CDP-ethanolamine:1,2-diacyl-sn-glycerol ethanolaminephosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-19-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-choline + 1,2-diacyl-sn-glycerol
CMP + a phosphatidylcholine
show the reaction diagram
-
-
-
r
CDP-ethanolamine + 1,2-diacyl-sn-glycerol
CMP + a phosphatidylethanolamine
show the reaction diagram
-
-
-
r
CDP-choline + 1,2-diacylglycerol
CMP + phosphatidylcholine
show the reaction diagram
CDP-choline + diacylglycerol
phosphatidylcholine + CMP
show the reaction diagram
only at CDP-choline concentrations higher than 25 micromolar, pH 8, 10 min, 37°C, in presence of 0.0002% Tween 20, 1 mM EGTA, 5 mM MnCl2
-
-
?
CDP-ethanolamine + 1,2-diacylglycerol
CMP + a phosphatidylethanolamine
show the reaction diagram
CDP-ethanolamine + diacylglycerol
phosphatidylethanolamine + CMP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-choline + 1,2-diacyl-sn-glycerol
CMP + a phosphatidylcholine
show the reaction diagram
-
-
-
r
CDP-ethanolamine + 1,2-diacyl-sn-glycerol
CMP + a phosphatidylethanolamine
show the reaction diagram
-
-
-
r
CDP-ethanolamine + diacylglycerol
phosphatidylethanolamine + CMP
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chelerythrine
-
protein kinase C inhibitor, IC50 0.04 mM
R59949
-
human DAG kinase inhibitor, IC50 0.04 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036 - 0.037
CDP-choline
0.0018 - 1800
CDP-ethanolamine
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
chelerythrine
Homo sapiens
-
protein kinase C inhibitor, IC50 0.04 mM
0.04
R59949
Homo sapiens
-
human DAG kinase inhibitor, IC50 0.04 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0763
5 mM Mn2+, pH 8, 37°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
deduced from primary structure
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
CEPT1 is elevated in diseased lower extremity arterial intima of individuals with peripheral arterial disease and diabetes
Manually annotated by BRENDA team
CEPT1 is elevated in carotid plaque of patients with diabetes
Manually annotated by BRENDA team
CEPT1 is elevated in diseased lower extremity arterial intima of individuals with peripheral arterial disease and diabetes
Manually annotated by BRENDA team
RT-PCR and Northern blot
Manually annotated by BRENDA team
low expression levels, revealed by RT-PCR and Northern blot
Manually annotated by BRENDA team
RT-PCR and Northern blot
Manually annotated by BRENDA team
RT-PCR and Northern blot
Manually annotated by BRENDA team
-
enzyme activity increases progressively through S and G2/M phases to a level that is 126% of unsynchronized cells
Manually annotated by BRENDA team
RT-PCR and Northern blot
Manually annotated by BRENDA team
RT-PCR and Northern blot
Manually annotated by BRENDA team
RT-PCR and Northern blot
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
an integral membrane protein
Manually annotated by BRENDA team
additional information
CPT/CEPT enzymes reside in multiple subcellular membranes that can move among organelles
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
reduction of phosphatidylcholine, e.g. by bacterial infection, results in apoptosis
metabolism
the CDP-choline and CDP-ethanolamine pathways are separate routes of phospholipid biosynthesis in mammalian cells, although the CEPT is probably a participant in both pathways. Physiological role of the CDP-choline pathway, overview
physiological function
CEPT1 is important for phosphatidylethanolamine formation from fatty acids such as 32:2, 32:1, 34:2, and 34:1. Brefeldin A treatment does not significantly affect the levels of the different phosphatidylethanolamine species. CEPT1 greatly prefers diacylglycerols 16:0-18:1 to other acceptors
physiological function
EPT1 is important for the de novo biosynthesis of 1-alkenyl-2-acyl-glycerophosphoethanolamine. EPT1 also contributed to the synthesis of phosphatidylethanolamine species containing the fatty acids 36:1, 36:4, 38:5, 38:4, 38:3, 40:6, 40:5, and 40:4. EPT1 prefers 1-alkyl-2-acyl-glycerol 16-20:4 over 1,2-diacylglycerol 18:0-20:4 over 1,2-diacylglycerol 16:0-18:1 and 1-alkyl-2-acyl-glycerol 16-18:1 species as lipid acceptors
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CEPT1_HUMAN
416
8
46554
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45180
deduced from primary structure
46500
-
x * 46500, SDS-PAGE and deduced from gene sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 46500, SDS-PAGE and deduced from gene sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
membrane precipitation by centrifugation a 100000g for 60 min
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
fom cDNA in pET23a for expression in Escherichia coli BL21 (DE3) pLysS
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of the gene encoding CPT, EC 2.7.8.2, but not CEPT increases during lipopolysaccahride-induced endoplasmic reticulum biogenesis in B-lymphocytes
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Henneberry, A.L.; McMaster, C.R.
Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine
Biochem. J.
339
291-298
1999
Homo sapiens
-
Manually annotated by BRENDA team
Wright, M.M.; McMaster, C.R.
PC and PE synthesis: mixed micellar analysis of the cholinephosphotransferase and ethanolaminephosphotransferase activities of human choline/ethanolamine phosphotransferase 1 (CEPT1)
Lipids
37
663-672
2002
Homo sapiens
Manually annotated by BRENDA team
Lin, W.; Arthur, G.
Phospholipids are synthesized in the G2/M phase of the cell cycle
Int. J. Biochem. Cell Biol.
39
597-605
2007
Homo sapiens
Manually annotated by BRENDA team
Horibata, Y.; Hirabayashi, Y.
Identification and characterization of human ethanolaminephosphotransferase1
J. Lipid Res.
48
503-508
2007
Homo sapiens (Q8WU57), Homo sapiens (Q9C0D9), Homo sapiens
Manually annotated by BRENDA team
Fagone, P.; Jackowski, S.
Phosphatidylcholine and the CDP-choline cycle
Biochim. Biophys. Acta
1831
523-532
2013
Homo sapiens (Q9Y6K0)
Manually annotated by BRENDA team
Zayed, M.A.; Jin, X.; Yang, C.; Belaygorod, L.; Engel, C.; Desai, K.; Harroun, N.; Saffaf, O.; Patterson, B.W.; Hsu, F.F.; Semenkovich, C.F.
CEPT1-mediated phospholipogenesis regulates endothelial cell function and ischemia-induced angiogenesis through PPARalpha
Diabetes
70
549-561
2020
Homo sapiens (Q9Y6K0), Homo sapiens, Mus musculus (Q8BGS7)
Manually annotated by BRENDA team
Horibata, Y.; Elpeleg, O.; Eran, A.; Hirabayashi, Y.; Savitzki, D.; Tal, G.; Mandel, H.; Sugimoto, H.
EPT1 (selenoprotein I) is critical for the neural development and maintenance of plasmalogen in humans
J. Lipid Res.
59
1015-1026
2018
Homo sapiens (Q9C0D9), Homo sapiens (Q9Y6K0), Homo sapiens
Manually annotated by BRENDA team