Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.7.B16 - DNA primase and Organism(s) Pyrococcus furiosus and UniProt Accession Q9P9H1

for references in articles please use BRENDA:EC2.7.7.B16
preliminary BRENDA-supplied EC number
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.B16 DNA primase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pyrococcus furiosus
UNIPROT: Q9P9H1
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Pyrococcus furiosus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
n
=
dN(pdN)n
+
n
+
n
=
N(pN)n
+
n
Synonyms
dna primase, dnag primase, polptn2, ssoprisl, prisl, pabp41, pabp46, pit3 replication protein, mjpri, more
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dNTP + n dNTP
dN(pdN)n + n diphosphate
show the reaction diagram
Q9P9H1; Q8U4H7
the p41-p46 primase preferentially uses dNTPs compared to NTPs. DNA strand synthesis is stimulated by the addition of NTPs or by addition of ATP to the same level. ATP is the preferable initiating nucleotide for the p41-p46 complex
-
-
?
NTP + n NTP
N(pN)n + n diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NTP + n NTP
N(pN)n + n diphosphate
show the reaction diagram
Q9P9H1; Q8U4H7
the p41-p46 complex synthesizes the primers, which can be extended in the combination with other replication proteins from Pyrococcus furiosus
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
Q9P9H1; Q8U4H7
the zinc ion is located within the loop region between beta-strand 6 and the prominent helix alpha4. It is coordinated by Cys 106 and His 108 of the loop and Cys114and Cys117 at the beginning of helix alpha4
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40772
Q9P9H1; Q8U4H7
1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence
46209
Q9P9H1; Q8U4H7
1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
Q9P9H1; Q8U4H7
1 * 46209 (large subunit Pfup46) + 1 * 40772 (small subunit Pfup41), calculated from sequence
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapor diffusion in sitting drops at 20°C. Crystal structure of the catalytic primase subunit, 2.3 A resolution
Q9P9H1; Q8U4H7
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the gene for the p58-like protein (gene product: Pfup46), expression in Escherichia coli
Q9P9H1; Q8U4H7
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, L.; Komori, K.; Ishino, S.; Bocquier, A.A.; Cann, I.K.; Kohda, D.; Ishino, Y.
The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus
J. Biol. Chem.
276
45484-45490
2001
Pyrococcus furiosus (Q9P9H1 and Q8U4H7), Pyrococcus furiosus
Manually annotated by BRENDA team
Augustin, M.A.; Huber, R.; Kaiser, J.T.
Crystal structure of a DNA-dependent RNA polymerase (DNA primase)
Nat. Struct. Biol.
8
57-61
2001
Pyrococcus furiosus (Q9P9H1 and Q8U4H7)
Manually annotated by BRENDA team