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Information on EC 2.7.7.9 - UTP-glucose-1-phosphate uridylyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9M9P3

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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9M9P3 not found.
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The taxonomic range for the selected organisms is: Arabidopsis thaliana
Synonyms
All3274, CugP, cyanobacterial UDP-Glc PPase, ExoN, GalU, Glc-1-P UTase, GlcNAc-1-P UTase, glucose 1-phosphate uridylyltransferase, glucose-1-phosphate uridylyltransferase, plastid UDP-glucose pyrophosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucose 1-phosphate uridylyltransferase
-
-
-
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glucose-1-phosphate uridylyltransferase
-
-
-
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plastid UDP-glucose pyrophosphorylase
302
-
UDP glucose pyrophosphorylase
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-
-
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UDP-glucose pyrophosphorylase
UDP-glucose pyrophosphorylase 1
302
-
UDP-glucose pyrophosphorylase UGP1
290983
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UDP-glucose pyrophosphorylase UGP2
288430
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UDP-glucose pyrophosphorylase UGP3
290983
-
UDPG phosphorylase
-
-
-
-
UDPG pyrophosphorylase
-
-
-
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UDPglucose pyrophosphorylase
-
-
-
-
UGP1
302
gene name
uridine 5'-diphosphoglucose pyrophosphorylase
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-
-
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uridine diphosphate-D-glucose pyrophosphorylase
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-
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uridine diphosphoglucose pyrophosphorylase
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-
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uridine-diphosphate glucose pyrophosphorylase
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-
-
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uridylyltransferase, glucose 1-phosphate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
UTP:alpha-D-glucose-1-phosphate uridylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-22-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
show the reaction diagram
additional information
?
-
-
regulation
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-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
additional information
?
-
-
regulation
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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absolute requirement for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diphosphate
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with 0.25 mM 57% activity, with 1 mM 11% activity, with 10 mM 1.5% activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sucrose
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activates; inducible by
additional information
-
enzyme expression is inducible by exogenous sucrose, light and cold stress
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
alpha-D-glucose 1-phosphate
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UDP-glucose synthesis
0.03
diphosphate
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pyrophosphorolysis
0.04
UDP-glucose
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pyrophosphorolysis
0.08
UTP
-
UDP-glucose synthesis
0.24 - 0.36
alpha-D-glucose 1-phosphate
0.07
diphosphate
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pyrophosphorolysis
0.06
UDP-glucose
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pyrophosphorolysis
0.031 - 0.17
UTP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
340
-
UDP-glucose synthesis
510
-
pyrophosphorolysis
385
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UDP-glucose synthesis
715
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pyrophosphorolysis
additional information
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the UGPase protein content and enzyme activity in the Pi-deficient mutant is about twice as high as in wild type and Pi-excess mutants, regardless of the light/dark conditions
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.14
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calculated for recombinant protein
6.1
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calculated for recombinant protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
UGP3 is a soluble plastid UDP-glucose pyrophosphorylase converts glucose-1-phosphate to UDP-glucose in the stroma
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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lack of sulfoquinovosyldiacylglycerol in the Arabidopsis ugp3 knockout mutant. The mutant does not show significant differences in glycosides and saccharides between wild type and ugp3 mutants
metabolism
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
UGPA2_ARATH
469
0
51738
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
-
53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da
53000
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53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da
monomer
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53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da
CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
uncomplexed enzyme and in presence of UTP and UDP-glucose. Structures show a carboxy-terminal beta-helix domain in a unique orientation. The nucleotide binding loop and the carboxy-terminal domain, including the possible catalytically important K360, move in and out of the active site in a concerted fashion
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion adsorption chromatography
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immobilized metal ion adsorption chromatography
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metal chelation chromatography
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expression in Agrobacterium tumefaciens
His-tagged version expressed in Escherichia coli BL21 (DE3)
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expression in Agrobacterium tumefaciens
His-tagged version expressed in Escherichia coli BL21 (DE3)
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His-tagged version expressed in Escherichia coli C41(DE3)
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UGP3 is highly coexpressed with SQD1 and SQD2
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
phosphate starvation
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UGP1 expression is up-regulated by sucrose in Arabidopsis leaves
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ciereszko, I.; Johansson, H.; Kleczkowski, L.A.
Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis
Biochem. J.
354
67-72
2001
Arabidopsis thaliana
Manually annotated by BRENDA team
Ciereszko, I.; Johansson, H.; Kleczkowski, L.A.
Interactive effects of phosphate deficiency, sucrose and light/dark conditions on gene expression of UDP-glucose pyrophosphorylase in Arabidopsis
J. Plant Physiol.
162
343-353
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
McCoy, J.G.; Bitto, E.; Bingman, C.A.; Wesenberg, G.E.; Bannen, R.M.; Kondrashov, D.A.; Phillips, G.N.
Structure and dynamics of UDP-glucose pyrophosphorylase from Arabidopsis thaliana with bound UDP-glucose and UTP
J. Mol. Biol.
366
830-841
2007
Arabidopsis thaliana, Arabidopsis thaliana (Q9M9P3)
Manually annotated by BRENDA team
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (P57751), Arabidopsis thaliana (Q9M9P3)
Manually annotated by BRENDA team
Meng, M.; Wilczynska, M.; Kleczkowski, L.A.
Molecular and kinetic characterization of two UDP-glucose pyrophosphorylases, products of distinct genes, from Arabidopsis
Biochim. Biophys. Acta
1784
967-972
2008
Arabidopsis thaliana (P57751), Arabidopsis thaliana (Q9M9P3)
Manually annotated by BRENDA team
Meng, M.; Geisler, M.; Johansson, H.; Harholt, J.; Scheller, H.V.; Mellerowicz, E.J.; Kleczkowski, L.A.
UDP-glucose pyrophosphorylase is not rate limiting, but is essential in Arabidopsis
Plant Cell Physiol.
50
998-1011
2009
Arabidopsis thaliana (P57751), Arabidopsis thaliana (Q94B70)
Manually annotated by BRENDA team
Okazaki, Y.; Shimojima, M.; Sawada, Y.; Toyooka, K.; Narisawa, T.; Mochida, K.; Tanaka, H.; Matsuda, F.; Hirai, A.; Hirai, M.Y.; Ohta, H.; Saito, K.
A chloroplastic UDP-glucose pyrophosphorylase from Arabidopsis is the committed enzyme for the first step of sulfolipid biosynthesis
Plant Cell
21
892-909
2009
Arabidopsis thaliana, Arabidopsis thaliana (Q94B70)
Manually annotated by BRENDA team
Chivasa, S.; Tome, D.F.; Slabas, A.R.
UDP-glucose pyrophosphorylase is a novel plant cell death regulator
J. Proteome Res.
12
1743-1753
2013
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Manually annotated by BRENDA team
Shimojima, M.
Biosynthesis and functions of the plant sulfolipid
Prog. Lipid Res.
50
234-239
2011
Arabidopsis thaliana, Spinacia oleracea
Manually annotated by BRENDA team
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