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Information on EC 2.7.7.87 - L-threonylcarbamoyladenylate synthase and Organism(s) Sulfurisphaera tokodaii and UniProt Accession Q970S6

for references in articles please use BRENDA:EC2.7.7.87
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IUBMB Comments
The enzyme is involved in the synthesis of N6-threonylcarbamoyladenosine37 in tRNAs, with the anticodon NNU, i.e. tRNAIle, tRNAThr, tRNAAsn, tRNALys, tRNASer and tRNAArg .
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Sulfurisphaera tokodaii
UNIPROT: Q970S6
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The taxonomic range for the selected organisms is: Sulfurisphaera tokodaii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sua5 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ATP:L-threonyl,bicarbonate adenylyltransferase
The enzyme is involved in the synthesis of N6-threonylcarbamoyladenosine37 in tRNAs, with the anticodon NNU, i.e. tRNAIle, tRNAThr, tRNAAsn, tRNALys, tRNASer and tRNAArg [6].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-threonine + ATP + bicarbonate
L-threonylcarbamoyladenylate + diphosphate + H2O
show the reaction diagram
determination of thermodynamic parameters of L-threonine, L-serine, ATP, ADP, AMP binding to Sua5 by ITC measurements
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38200
determined by analytical ultracentrifugation at 20°C and at 25°C
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 38200, analytical ultracentrifugation, Sulfolobus tokodaii Sua5 exists as a monomer in solution
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals grown at 30°C by sitting drop method, complexed with L-threonine and AMPPNP at 1.8 A resolution
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Sulfolobus tokodaii Sua5 protein overexpressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kuratani, M.; Kasai, T.; Akasaka, R.; Higashijima, K.; Terada, T.; Kigawa, T.; Shinkai, A.; Bessho, Y.; Yokoyama, S.
Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNP.
Proteins
79
2065-2075
2011
Sulfurisphaera tokodaii (Q970S6)
Manually annotated by BRENDA team