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Synonyms
polynucleotide phosphorylase, hpnpase(old-35), pnpt1, polyribonucleotide nucleotidyltransferase, atmtpnpase, cppnpase, hpnpaseold-35, chloroplast pnpase, polyribonucleotide phosphorylase,
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AtcpPNPase
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i.e. Arabidopsis thaliana chloroplast PNPase
nucleoside diphosphate:polynucleotidyl transferase
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nucleotidyltransferase, polyribonucleotide
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polynucleotide phosphorylase
polyribonucleotide phosphorylase
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chloroplast PNPase
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cpPNPase
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hPNPaseold-35
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PNPase
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PNPT1
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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polynucleotide phosphorylase
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RNase PH
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
stem-loops of 7,9 or 11 bp block the processive 3'-5' exonuclease action of PNPase
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
mechanism of elongation, overview
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
PNPase exhibits 3'-to-5' exonucleolytic activity
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
PNPase exhibits 3'-to-5' exonucleolytic activity
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RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
analysis of the different PNPase domains for polymerization, degradation, and RNA binding properties
RNAn+1 + phosphate = RNAn + a nucleoside diphosphate
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2',3'-cyclic RNAn + phosphate
RNA(n-1) + a nucleotide diphosphate
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?
24-nucleotide RNA molecule + ADP
25-nucleotide RNA molecule + phosphate
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when both ADP and phosphate are present at the reaction mixture, the direction of activity, either polyadenylation or degradation, is dependent on their relative concentrations
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r
24-nucleotide RNA molecule + phosphate
23-nucleotide RNA molecule + nucleoside diphosphate
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when both ADP and phosphate are present at the reaction mixture, the direction of activity, either polyadenylation or degradation, is dependent on their relative concentrations
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r
3'-phosphorylated RNA(n) + phosphate
RNA(n-1) + a nucleotide diphosphate
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enzyme inefficiently degrades 3'-phosphorylated RNA
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?
ADP + globin mRNAn
globin mRNAn+1 + phosphate
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?
microR-221 RNAn+1 + phosphate
microR-221 RNAn + ADP
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recombinantly expressed microRNAs miR-let7a, miR-106b, miR-25, miR-221, miR-222, and miR-184 as substrates, the recombinant enzyme selectively and preferentially degrades microRNA-221 in human melanoma cells
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r
microRNAn+1 + phosphate
microRNAn + ADP
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recombinantly expressed microRNAs miR-let7a, miR-106b, miR-25, miR-221, miR-222, and miR-184
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r
pJFD4 HpaI RNA+1 + phosphate
pJFD4 HpaI RNA + nucleoside diphosphate
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derivative of SP82 phage RNA, arsenate can replace phosphate
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?
poly(A) + ADP
poly(A)+1 + phosphate
poly(A)(n-1) + ADP
poly(A)n + phosphate
poly(A)+1 + phosphate
poly(A) + ADP
poly(A)n + phosphate
poly(A)(n-1) + ADP
poly(C) + CDP
poly(C)+1 + phosphate
poly(C)+1 + phosphate
poly(C) + ADP
poly(G) + GDP
poly(G)+1 + phosphate
poly(I) + IDP
poly(I)+1 + phosphate
poly(U)+ UDP
poly(U)+1 + phosphate
poly(U)+1 + phosphate
poly(U) + ADP
polyadenylic acid + phosphate
? + ADP
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?
polycytidylic acid + phosphate
? + CDP
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polyguanylic acid + phosphate
? + GDP
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?
polyuridylic acid + phosphate
? + UDP
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?
rabbit globin mRNAn+1 + phosphate
ADP + rabbit globin mRNAn
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only the poly(A) tail of the mRNA is removed
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?
ribonucleoside 5'-diphosphate + phosphate
ribonucleoside 5'-diphosphate + phosphate
RNA + ATP
RNA+1 + diphosphate
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polymerization in the absence of phosphate
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r
RNA + CTP
RNA+1 + diphosphate
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polymerization in the absence of phosphate
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r
RNA + GTP
RNA+1 + diphosphate
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polymerization in the absence of phosphate
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r
RNA + UTP
RNA+1 + diphosphate
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polymerization in the absence of phosphate
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r
RNA(n) + phosphate
RNA(n-1) + a nucleotide diphosphate
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?
RNAn + a nucleoside diphosphate
RNAn+1 + phosphate
RNAn + UDP
RNAn+1 + phosphate
RNAn+1 + phosphate
RNAn + a nucleoside diphosphate
yeast RNA+1 + phosphate
yeast RNA + nucleoside diphosphate
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2% of activity with poly(U)
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?
additional information
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poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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no phosphorolysis activity with poly(G)
?
poly(A) + ADP
poly(A)+1 + phosphate
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no phosphorolysis activity with poly(G)
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poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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?
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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strong preference for ADP and poly(A) in phosphorolysis and polymerization reaction
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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?
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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poly(A) polymerization product containing 8000-13000 nucleotides
r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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32% of activity with poly(U) in phosphorolysis reaction
r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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chloroplast PNPase has both exonuclease and poly(A) polymerase activity, phosphate enhances RNA degradation activity, ADP inhibits degradation and enhances poly(A) polymerization, ADP best substrate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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r
poly(A) + ADP
poly(A)+1 + phosphate
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primer required for polymerization
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r
poly(A)(n-1) + ADP
poly(A)n + phosphate
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r
poly(A)(n-1) + ADP
poly(A)n + phosphate
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r
poly(A)(n-1) + ADP
poly(A)n + phosphate
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r
poly(A)+1 + phosphate
poly(A) + ADP
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r
poly(A)+1 + phosphate
poly(A) + ADP
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r
poly(A)+1 + phosphate
poly(A) + ADP
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ir
poly(A)+1 + phosphate
poly(A) + ADP
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r
poly(A)n + phosphate
poly(A)(n-1) + ADP
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r
poly(A)n + phosphate
poly(A)(n-1) + ADP
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r
poly(A)n + phosphate
poly(A)(n-1) + ADP
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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51% of activity with ADP
21% of activity with poly(U) in phosphorolysis reaction
r
poly(C) + CDP
poly(C)+1 + phosphate
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low poly(C) phosphorolysis activity
r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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r
poly(C) + CDP
poly(C)+1 + phosphate
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primer required for polymerization
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r
poly(C)+1 + phosphate
poly(C) + ADP
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ir
poly(C)+1 + phosphate
poly(C) + ADP
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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much lower activity than with ADP, activity depends on polyribonucleotide primer
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?
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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very little activity
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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10% of activity with ADP and poly(A)
less than 15% of phosphorolysis activity with poly(A)
r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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GDP second best substrate
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?
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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r
poly(G) + GDP
poly(G)+1 + phosphate
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primer required for polymerization
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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?
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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phosphorolysis at 14% of activity with poly(A)
?
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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48% of activity with ADP
28% of activity with poly(U) in phosphorolysis reaction
r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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r
poly(I) + IDP
poly(I)+1 + phosphate
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primer required for polymerization
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r
poly(U)+ UDP
poly(U)+1 + phosphate
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r
poly(U)+ UDP
poly(U)+1 + phosphate
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r
poly(U)+ UDP
poly(U)+1 + phosphate
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55% of activity with ADP
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r
poly(U)+ UDP
poly(U)+1 + phosphate
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best substrate for phosphorolysis reaction
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r
poly(U)+ UDP
poly(U)+1 + phosphate
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lower activity than with poly(A)
r
poly(U)+ UDP
poly(U)+1 + phosphate
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r
poly(U)+ UDP
poly(U)+1 + phosphate
2.7.7.8 polyribonucleotide nucleotidyltransferase
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