Information on EC 2.7.7.78 - GDP-D-glucose phosphorylase

for references in articles please use BRENDA:EC2.7.7.78
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.78
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RECOMMENDED NAME
GeneOntology No.
GDP-D-glucose phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-alpha-D-glucose + phosphate = alpha-D-glucose 1-phosphate + GDP
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
GDP:alpha-D-glucose 1-phosphate guanylyltransferase
The enzyme may be involved in prevention of misincorporation of glucose in place of mannose residues into glycoconjugates i.e. to remove accidentally produced GDP-alpha-D-glucose. Activities with GDP-L-galactose, GDP-D-mannose and UDP-D-glucose are all less than 3% that with GDP-D-glucose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-alpha-D-glucose + phosphate
alpha-D-glucose 1-phosphate + GDP
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-alpha-D-glucose + phosphate
alpha-D-glucose 1-phosphate + GDP
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 0.01
GDP-alpha-D-glucose
1.2 - 2.9
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26 - 265
GDP-alpha-D-glucose
28.1 - 380
phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1300 - 26500
GDP-alpha-D-glucose
9.7 - 317
phosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest levels measured in brain and testis (4-8 times higher than in the remaining tissues)
Manually annotated by BRENDA team
highest levels measured in brain and testis (4-8 times higher than in the remaining tissues)
Manually annotated by BRENDA team
highest levels measured in brain and testis (4-8 times higher than in the remaining tissues)
Manually annotated by BRENDA team
expressed in all the normal larval stages observed
Manually annotated by BRENDA team
highest levels measured in brain and testis (4-8 times higher than in the remaining tissues)
Manually annotated by BRENDA team
highest levels measured in brain and testis (4-8 times higher than in the remaining tissues)
Manually annotated by BRENDA team
highest levels measured in brain and testis (4-8 times higher than in the remaining tissues)
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 10% glycerol
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli