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Information on EC 2.7.7.70 - D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase and Organism(s) Escherichia coli and UniProt Accession P76658

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IUBMB Comments
The bifunctional protein hldE includes D-glycero-beta-D-manno-heptose-7-phosphate kinase and D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase activity (cf. EC 2.7.1.167). The enzyme is involved in biosynthesis of ADP-L-glycero-beta-D-manno-heptose, which is utilized for assembly of the lipopolysaccharide inner core in Gram-negative bacteria.
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Escherichia coli
UNIPROT: P76658
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
bifunctional D-beta-D-heptose-7-phosphate kinase/D-beta-dheptose-1-phosphate adenylyltransferase, D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase, D-glycero-beta-D-manno-heptose-1-phosphate adenylyltransferase, D-glycero-D-manno-heptose-1beta-phosphate adenylyltransferase, HldC, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase
bifunctional enzyme
bifunctional D-beta-D-heptose-7-phosphate kinase/D-beta-dheptose-1-phosphate adenylyltransferase
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
The bifunctional protein hldE includes D-glycero-beta-D-manno-heptose-7-phosphate kinase and D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase activity (cf. EC 2.7.1.167). The enzyme is involved in biosynthesis of ADP-L-glycero-beta-D-manno-heptose, which is utilized for assembly of the lipopolysaccharide inner core in Gram-negative bacteria.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
ADP-D-glycero-beta-D-manno-heptose + diphosphate
show the reaction diagram
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
ADP-D-glycero-beta-D-manno-heptose + diphosphate
show the reaction diagram
additional information
?
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rfaE encodes a bifunctional protein. It is proposed that domain I is involved in the synthesis of D-glycero-D-manno-heptose 1-phosphate, whereas domain II catalyzes the ADP transfer to form ADP-D-glycero-D-manno-heptose
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
ADP-D-glycero-beta-D-manno-heptose + diphosphate
show the reaction diagram
synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase)
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?
D-glycero-beta-D-manno-heptose 1-phosphate + ATP
ADP-D-glycero-beta-D-manno-heptose + diphosphate
show the reaction diagram
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?
additional information
?
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rfaE encodes a bifunctional protein. It is proposed that domain I is involved in the synthesis of D-glycero-D-manno-heptose 1-phosphate, whereas domain II catalyzes the ADP transfer to form ADP-D-glycero-D-manno-heptose
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional protein hldE includes: D-beta-D-heptose 7-phosphate kinase and D-beta-D-heptose 1-phosphate adenosyltransferase
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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x * 55000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 55000, SDS-PAGE
additional information
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the N-terminal domain I spans amino acids 1-318 and shares structural features with members of the ribokinase family. The C-terminal domain II, which spans amino acids 344-477, has all the conserved features of the cytidylyltransferase superfamily
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Valvano, M.A.; Marolda, C.L.; Bittner, M.; Glaskin-Clay, M.; Simon, T.L.; Klena, J.D.
The rfaE gene from Escherichia coli encodes a bifunctional protein involved in biosynthesis of the lipopolysaccharide core precursor ADP-L-glycero-D-manno-heptose
J. Bacteriol.
182
488-497
2000
Escherichia coli (P76658), Salmonella enterica subsp. enterica serovar Typhimurium (Q7CPR9)
Manually annotated by BRENDA team
Kneidinger, B.; Marolda, C.; Graninger, M.; Zamyatina, A.; McArthur, F.; Kosma, P.; Valvano, M.A.; Messner, P.
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli
J. Bacteriol.
184
363-369
2002
Escherichia coli (P76658)
Manually annotated by BRENDA team
Valvano, M.A.; Messner, P.; Kosma, P.
Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides
Microbiology
148
1979-1989
2002
Escherichia coli
Manually annotated by BRENDA team